Nkolbisson virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ledantevirus; Nkolbisson ledantevirus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R1P3|A0A0D3R1P3_9RHAB Glycoprotein OS=Nkolbisson virus OX=380442 PE=4 SV=1
MM1 pKa = 7.23SRR3 pKa = 11.84QRR5 pKa = 11.84LRR7 pKa = 11.84EE8 pKa = 3.33IAGRR12 pKa = 11.84YY13 pKa = 9.19DD14 pKa = 3.46YY15 pKa = 11.8SRR17 pKa = 11.84VAKK20 pKa = 10.21NLEE23 pKa = 3.79GLEE26 pKa = 3.83EE27 pKa = 4.45DD28 pKa = 3.93EE29 pKa = 5.1NEE31 pKa = 4.43KK32 pKa = 10.66EE33 pKa = 4.04LEE35 pKa = 4.19AAAGTIPANIVEE47 pKa = 4.73EE48 pKa = 4.39PDD50 pKa = 3.43SSEE53 pKa = 5.48DD54 pKa = 3.61EE55 pKa = 4.79ASDD58 pKa = 3.64GDD60 pKa = 3.9EE61 pKa = 4.51EE62 pKa = 4.05EE63 pKa = 4.47LEE65 pKa = 4.25RR66 pKa = 11.84IFRR69 pKa = 11.84EE70 pKa = 4.18DD71 pKa = 3.2EE72 pKa = 3.94KK73 pKa = 11.6SEE75 pKa = 4.51DD76 pKa = 3.7EE77 pKa = 4.69LEE79 pKa = 4.22NDD81 pKa = 3.31HH82 pKa = 7.39FEE84 pKa = 4.45LSLEE88 pKa = 4.17AEE90 pKa = 4.43EE91 pKa = 4.28EE92 pKa = 4.39AEE94 pKa = 4.92LEE96 pKa = 4.23SSSDD100 pKa = 3.23KK101 pKa = 11.07TIILPPMPPLSGLRR115 pKa = 11.84YY116 pKa = 9.33QDD118 pKa = 3.34EE119 pKa = 4.52LEE121 pKa = 4.44NIILHH126 pKa = 5.32YY127 pKa = 10.78VSTSLASVGWYY138 pKa = 10.28LNPQQIEE145 pKa = 4.09KK146 pKa = 10.95GEE148 pKa = 4.13GGLRR152 pKa = 11.84VGINKK157 pKa = 8.32ITDD160 pKa = 3.57NQPLSSLDD168 pKa = 3.2TTAPEE173 pKa = 3.78EE174 pKa = 4.22SLDD177 pKa = 4.01VVIPDD182 pKa = 4.1DD183 pKa = 4.82LIKK186 pKa = 10.94EE187 pKa = 4.29EE188 pKa = 4.58EE189 pKa = 4.36DD190 pKa = 3.58TVTRR194 pKa = 11.84PDD196 pKa = 2.96VDD198 pKa = 3.34KK199 pKa = 11.23LFRR202 pKa = 11.84EE203 pKa = 4.7GLSFTRR209 pKa = 11.84KK210 pKa = 8.4GNKK213 pKa = 9.81GIIEE217 pKa = 4.45VNSEE221 pKa = 3.94TPGWSKK227 pKa = 10.98EE228 pKa = 3.85LLEE231 pKa = 4.93FATTMFGNRR240 pKa = 11.84QDD242 pKa = 3.77QIDD245 pKa = 4.43YY246 pKa = 10.9ILDD249 pKa = 3.77EE250 pKa = 5.1LEE252 pKa = 4.58LLPMIKK258 pKa = 9.87RR259 pKa = 11.84LCIYY263 pKa = 9.7PP264 pKa = 3.72

Molecular weight: 29.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R205|A0A0D3R205_9RHAB Nucleocapsid protein OS=Nkolbisson virus OX=380442 PE=4 SV=1
MM1 pKa = 7.06QLSLLMVMASLAQSVTGRR19 pKa = 11.84AAGRR23 pKa = 11.84EE24 pKa = 3.97LHH26 pKa = 6.97DD27 pKa = 4.28VIGFVPDD34 pKa = 3.47TDD36 pKa = 3.75TLQWKK41 pKa = 8.1PANLDD46 pKa = 3.38TLRR49 pKa = 11.84CPEE52 pKa = 4.4AADD55 pKa = 4.45PGPDD59 pKa = 3.38DD60 pKa = 3.96GAVVEE65 pKa = 4.46KK66 pKa = 10.2WVISRR71 pKa = 11.84PRR73 pKa = 11.84VNTFLGIKK81 pKa = 10.25GYY83 pKa = 10.14LCHH86 pKa = 5.71YY87 pKa = 8.43AKK89 pKa = 10.19WVTRR93 pKa = 11.84CEE95 pKa = 4.19YY96 pKa = 9.51TWYY99 pKa = 10.45LSKK102 pKa = 10.02TISRR106 pKa = 11.84SIQPLDD112 pKa = 3.53VTEE115 pKa = 5.09SGCQDD120 pKa = 2.81AFKK123 pKa = 10.47EE124 pKa = 4.04YY125 pKa = 10.61DD126 pKa = 3.15AGRR129 pKa = 11.84LVPGSFPPEE138 pKa = 3.44ACYY141 pKa = 9.05WASTNDD147 pKa = 3.9EE148 pKa = 4.26TVHH151 pKa = 6.83AYY153 pKa = 8.77TITPHH158 pKa = 5.6EE159 pKa = 4.74VIYY162 pKa = 10.71DD163 pKa = 3.99PYY165 pKa = 11.57SNAKK169 pKa = 9.31LDD171 pKa = 4.09HH172 pKa = 6.56IFLHH176 pKa = 6.61GSCNKK181 pKa = 9.9DD182 pKa = 3.17FCEE185 pKa = 4.24TSHH188 pKa = 7.58DD189 pKa = 3.75STMWIAKK196 pKa = 9.66RR197 pKa = 11.84KK198 pKa = 10.3GEE200 pKa = 3.99GSVCQFVGRR209 pKa = 11.84EE210 pKa = 3.85DD211 pKa = 3.54VEE213 pKa = 4.39IIEE216 pKa = 4.34GAKK219 pKa = 10.04FSLRR223 pKa = 11.84KK224 pKa = 9.27FQTKK228 pKa = 7.76VWLRR232 pKa = 11.84GPSLHH237 pKa = 6.99NIPLDD242 pKa = 3.69KK243 pKa = 10.67LCKK246 pKa = 9.22IDD248 pKa = 3.94FCGKK252 pKa = 8.17TGYY255 pKa = 10.36RR256 pKa = 11.84NQQGIWFSIDD266 pKa = 2.78RR267 pKa = 11.84VSWSKK272 pKa = 10.74NHH274 pKa = 6.38SLPIRR279 pKa = 11.84QKK281 pKa = 11.24AVGCKK286 pKa = 9.37PGEE289 pKa = 4.09NVAVVDD295 pKa = 4.14TNLPDD300 pKa = 3.92SDD302 pKa = 4.64LEE304 pKa = 4.17ATIEE308 pKa = 3.98EE309 pKa = 4.71MMWDD313 pKa = 3.99MNCLNALEE321 pKa = 4.96TIQHH325 pKa = 5.67HH326 pKa = 6.42KK327 pKa = 10.35KK328 pKa = 10.75VSLHH332 pKa = 6.82DD333 pKa = 4.72LYY335 pKa = 11.26QIAPQKK341 pKa = 10.4SGPGLAYY348 pKa = 10.61RR349 pKa = 11.84LNDD352 pKa = 3.18GHH354 pKa = 8.81LEE356 pKa = 4.17VAQAHH361 pKa = 5.54YY362 pKa = 10.3WSVQHH367 pKa = 6.36PKK369 pKa = 10.1RR370 pKa = 11.84GKK372 pKa = 10.35LSRR375 pKa = 11.84TCLGARR381 pKa = 11.84QADD384 pKa = 3.98EE385 pKa = 4.08TSSCVKK391 pKa = 8.48WNKK394 pKa = 6.02WTHH397 pKa = 4.47VANNTYY403 pKa = 10.19HH404 pKa = 6.67AVNGITEE411 pKa = 4.08VDD413 pKa = 3.76GQILFPKK420 pKa = 10.03RR421 pKa = 11.84RR422 pKa = 11.84VLHH425 pKa = 6.24RR426 pKa = 11.84KK427 pKa = 9.2YY428 pKa = 10.61DD429 pKa = 3.52PEE431 pKa = 4.04YY432 pKa = 10.31ATRR435 pKa = 11.84QSLRR439 pKa = 11.84FIKK442 pKa = 10.58HH443 pKa = 4.53PVIDD447 pKa = 3.89KK448 pKa = 11.15FEE450 pKa = 4.8DD451 pKa = 3.41KK452 pKa = 10.17ATEE455 pKa = 4.34NIEE458 pKa = 4.11HH459 pKa = 7.29KK460 pKa = 10.73DD461 pKa = 3.57IISKK465 pKa = 10.34DD466 pKa = 3.58VNAGDD471 pKa = 5.2LVGNWVVVAEE481 pKa = 4.83GKK483 pKa = 9.6LSEE486 pKa = 4.2WFGGLGKK493 pKa = 10.7SIISVVSLIVGLLVLYY509 pKa = 9.43VIVKK513 pKa = 8.87LCLYY517 pKa = 8.55FRR519 pKa = 11.84PKK521 pKa = 10.6AKK523 pKa = 10.34EE524 pKa = 3.88KK525 pKa = 10.63IGDD528 pKa = 3.63MEE530 pKa = 4.35MKK532 pKa = 10.23LKK534 pKa = 10.46KK535 pKa = 9.98RR536 pKa = 11.84SEE538 pKa = 4.33SFGG541 pKa = 3.45

Molecular weight: 61.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3550

210

2114

710.0

81.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.732 ± 0.849

2.113 ± 0.254

6.254 ± 0.277

7.183 ± 1.226

3.915 ± 0.417

6.0 ± 0.441

2.704 ± 0.315

6.394 ± 0.45

6.394 ± 0.346

9.972 ± 0.395

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.294

4.197 ± 0.238

4.563 ± 0.537

3.127 ± 0.083

5.746 ± 0.331

7.211 ± 0.764

5.324 ± 0.186

6.31 ± 0.532

1.859 ± 0.225

3.577 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski