Amino acid dipepetide frequency for Pectobacterium phage PP74

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.002AlaAla: 10.002 ± 1.538
0.661AlaCys: 0.661 ± 0.315
5.869AlaAsp: 5.869 ± 0.662
6.943AlaGlu: 6.943 ± 0.788
3.306AlaPhe: 3.306 ± 0.563
7.605AlaGly: 7.605 ± 1.258
1.488AlaHis: 1.488 ± 0.307
5.703AlaIle: 5.703 ± 0.784
6.861AlaLys: 6.861 ± 0.921
7.357AlaLeu: 7.357 ± 0.865
2.893AlaMet: 2.893 ± 0.449
3.802AlaAsn: 3.802 ± 0.604
3.141AlaPro: 3.141 ± 0.497
3.472AlaGln: 3.472 ± 0.503
4.712AlaArg: 4.712 ± 0.535
5.869AlaSer: 5.869 ± 1.101
4.712AlaThr: 4.712 ± 0.893
5.455AlaVal: 5.455 ± 0.843
1.24AlaTrp: 1.24 ± 0.276
2.893AlaTyr: 2.893 ± 0.534
0.0AlaXaa: 0.0 ± 0.0
Cys
1.157CysAla: 1.157 ± 0.312
0.165CysCys: 0.165 ± 0.188
0.744CysAsp: 0.744 ± 0.326
0.331CysGlu: 0.331 ± 0.157
0.413CysPhe: 0.413 ± 0.202
0.579CysGly: 0.579 ± 0.241
0.579CysHis: 0.579 ± 0.236
0.413CysIle: 0.413 ± 0.209
0.413CysLys: 0.413 ± 0.257
0.909CysLeu: 0.909 ± 0.306
0.248CysMet: 0.248 ± 0.17
0.413CysAsn: 0.413 ± 0.25
0.413CysPro: 0.413 ± 0.187
0.661CysGln: 0.661 ± 0.256
0.248CysArg: 0.248 ± 0.202
0.827CysSer: 0.827 ± 0.334
0.331CysThr: 0.331 ± 0.193
0.496CysVal: 0.496 ± 0.255
0.248CysTrp: 0.248 ± 0.151
0.248CysTyr: 0.248 ± 0.212
0.0CysXaa: 0.0 ± 0.0
Asp
4.464AspAla: 4.464 ± 0.605
0.827AspCys: 0.827 ± 0.328
4.959AspAsp: 4.959 ± 0.893
3.968AspGlu: 3.968 ± 0.548
2.728AspPhe: 2.728 ± 0.41
6.613AspGly: 6.613 ± 0.554
0.909AspHis: 0.909 ± 0.247
3.141AspIle: 3.141 ± 0.4
4.133AspLys: 4.133 ± 0.73
3.554AspLeu: 3.554 ± 0.516
2.728AspMet: 2.728 ± 0.666
2.728AspAsn: 2.728 ± 0.385
3.058AspPro: 3.058 ± 0.517
1.818AspGln: 1.818 ± 0.347
2.562AspArg: 2.562 ± 0.516
4.464AspSer: 4.464 ± 0.581
3.968AspThr: 3.968 ± 0.475
4.216AspVal: 4.216 ± 0.418
0.661AspTrp: 0.661 ± 0.303
1.818AspTyr: 1.818 ± 0.301
0.0AspXaa: 0.0 ± 0.0
Glu
7.522GluAla: 7.522 ± 0.985
0.909GluCys: 0.909 ± 0.303
4.05GluAsp: 4.05 ± 0.641
5.455GluGlu: 5.455 ± 1.084
2.728GluPhe: 2.728 ± 0.49
4.959GluGly: 4.959 ± 0.601
1.901GluHis: 1.901 ± 0.508
3.389GluIle: 3.389 ± 0.544
2.81GluLys: 2.81 ± 0.485
5.621GluLeu: 5.621 ± 0.654
3.058GluMet: 3.058 ± 0.528
3.389GluAsn: 3.389 ± 0.565
2.314GluPro: 2.314 ± 0.417
3.389GluGln: 3.389 ± 0.492
4.133GluArg: 4.133 ± 0.544
3.554GluSer: 3.554 ± 0.573
2.976GluThr: 2.976 ± 0.397
4.546GluVal: 4.546 ± 0.536
1.405GluTrp: 1.405 ± 0.355
2.893GluTyr: 2.893 ± 0.453
0.0GluXaa: 0.0 ± 0.0
Phe
2.976PheAla: 2.976 ± 0.518
0.331PheCys: 0.331 ± 0.189
1.984PheAsp: 1.984 ± 0.487
2.314PheGlu: 2.314 ± 0.419
0.909PhePhe: 0.909 ± 0.273
2.893PheGly: 2.893 ± 0.553
0.827PheHis: 0.827 ± 0.283
1.571PheIle: 1.571 ± 0.342
3.141PheLys: 3.141 ± 0.508
2.728PheLeu: 2.728 ± 0.367
1.157PheMet: 1.157 ± 0.297
1.736PheAsn: 1.736 ± 0.479
1.736PhePro: 1.736 ± 0.427
0.992PheGln: 0.992 ± 0.274
2.48PheArg: 2.48 ± 0.42
1.818PheSer: 1.818 ± 0.38
2.562PheThr: 2.562 ± 0.415
2.066PheVal: 2.066 ± 0.355
0.413PheTrp: 0.413 ± 0.199
0.909PheTyr: 0.909 ± 0.294
0.0PheXaa: 0.0 ± 0.0
Gly
6.778GlyAla: 6.778 ± 0.998
0.744GlyCys: 0.744 ± 0.301
5.703GlyAsp: 5.703 ± 0.828
5.703GlyGlu: 5.703 ± 0.701
2.314GlyPhe: 2.314 ± 0.461
6.282GlyGly: 6.282 ± 0.813
0.744GlyHis: 0.744 ± 0.273
4.216GlyIle: 4.216 ± 0.65
6.53GlyLys: 6.53 ± 0.855
7.357GlyLeu: 7.357 ± 0.995
2.314GlyMet: 2.314 ± 0.512
2.645GlyAsn: 2.645 ± 0.395
0.413GlyPro: 0.413 ± 0.181
3.058GlyGln: 3.058 ± 0.392
3.885GlyArg: 3.885 ± 0.52
5.042GlySer: 5.042 ± 0.675
3.554GlyThr: 3.554 ± 0.498
4.464GlyVal: 4.464 ± 0.636
2.149GlyTrp: 2.149 ± 0.483
3.224GlyTyr: 3.224 ± 0.464
0.0GlyXaa: 0.0 ± 0.0
His
0.909HisAla: 0.909 ± 0.37
0.331HisCys: 0.331 ± 0.18
0.661HisAsp: 0.661 ± 0.226
1.488HisGlu: 1.488 ± 0.327
0.909HisPhe: 0.909 ± 0.269
1.736HisGly: 1.736 ± 0.381
0.413HisHis: 0.413 ± 0.178
1.571HisIle: 1.571 ± 0.305
1.488HisLys: 1.488 ± 0.412
1.901HisLeu: 1.901 ± 0.382
0.661HisMet: 0.661 ± 0.252
1.075HisAsn: 1.075 ± 0.323
0.248HisPro: 0.248 ± 0.116
0.331HisGln: 0.331 ± 0.168
0.827HisArg: 0.827 ± 0.247
1.075HisSer: 1.075 ± 0.277
0.992HisThr: 0.992 ± 0.255
1.157HisVal: 1.157 ± 0.318
0.331HisTrp: 0.331 ± 0.149
0.661HisTyr: 0.661 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
4.381IleAla: 4.381 ± 0.672
0.496IleCys: 0.496 ± 0.247
3.389IleAsp: 3.389 ± 0.458
3.306IleGlu: 3.306 ± 0.435
0.909IlePhe: 0.909 ± 0.27
3.885IleGly: 3.885 ± 0.401
1.24IleHis: 1.24 ± 0.355
2.232IleIle: 2.232 ± 0.556
3.802IleLys: 3.802 ± 0.603
3.72IleLeu: 3.72 ± 0.514
1.157IleMet: 1.157 ± 0.301
3.058IleAsn: 3.058 ± 0.63
2.066IlePro: 2.066 ± 0.459
1.488IleGln: 1.488 ± 0.4
3.306IleArg: 3.306 ± 0.395
3.141IleSer: 3.141 ± 0.562
2.893IleThr: 2.893 ± 0.594
2.562IleVal: 2.562 ± 0.527
0.661IleTrp: 0.661 ± 0.195
1.984IleTyr: 1.984 ± 0.497
0.0IleXaa: 0.0 ± 0.0
Lys
7.522LysAla: 7.522 ± 0.894
0.661LysCys: 0.661 ± 0.224
4.381LysAsp: 4.381 ± 0.575
4.959LysGlu: 4.959 ± 0.56
2.314LysPhe: 2.314 ± 0.466
5.125LysGly: 5.125 ± 0.752
1.571LysHis: 1.571 ± 0.369
2.232LysIle: 2.232 ± 0.342
4.959LysLys: 4.959 ± 0.956
6.53LysLeu: 6.53 ± 0.834
2.066LysMet: 2.066 ± 0.319
2.562LysAsn: 2.562 ± 0.391
2.48LysPro: 2.48 ± 0.635
2.48LysGln: 2.48 ± 0.479
3.389LysArg: 3.389 ± 0.554
4.133LysSer: 4.133 ± 0.63
3.389LysThr: 3.389 ± 0.413
4.464LysVal: 4.464 ± 0.48
0.579LysTrp: 0.579 ± 0.217
2.149LysTyr: 2.149 ± 0.37
0.0LysXaa: 0.0 ± 0.0
Leu
8.927LeuAla: 8.927 ± 1.117
0.496LeuCys: 0.496 ± 0.263
4.298LeuAsp: 4.298 ± 0.443
5.786LeuGlu: 5.786 ± 1.003
2.397LeuPhe: 2.397 ± 0.454
4.133LeuGly: 4.133 ± 0.638
1.323LeuHis: 1.323 ± 0.328
4.05LeuIle: 4.05 ± 0.56
6.53LeuLys: 6.53 ± 0.727
5.125LeuLeu: 5.125 ± 0.909
2.48LeuMet: 2.48 ± 0.478
3.802LeuAsn: 3.802 ± 0.653
2.48LeuPro: 2.48 ± 0.464
3.637LeuGln: 3.637 ± 0.597
6.034LeuArg: 6.034 ± 0.648
4.546LeuSer: 4.546 ± 0.594
4.464LeuThr: 4.464 ± 0.602
5.29LeuVal: 5.29 ± 0.571
1.405LeuTrp: 1.405 ± 0.434
2.149LeuTyr: 2.149 ± 0.425
0.0LeuXaa: 0.0 ± 0.0
Met
2.893MetAla: 2.893 ± 0.416
0.165MetCys: 0.165 ± 0.119
1.984MetAsp: 1.984 ± 0.47
2.232MetGlu: 2.232 ± 0.475
1.24MetPhe: 1.24 ± 0.301
2.728MetGly: 2.728 ± 0.459
0.331MetHis: 0.331 ± 0.187
1.24MetIle: 1.24 ± 0.27
1.736MetLys: 1.736 ± 0.312
3.554MetLeu: 3.554 ± 0.459
0.827MetMet: 0.827 ± 0.252
1.736MetAsn: 1.736 ± 0.367
1.571MetPro: 1.571 ± 0.391
1.488MetGln: 1.488 ± 0.38
1.323MetArg: 1.323 ± 0.342
2.232MetSer: 2.232 ± 0.336
1.901MetThr: 1.901 ± 0.39
1.736MetVal: 1.736 ± 0.423
0.165MetTrp: 0.165 ± 0.125
0.827MetTyr: 0.827 ± 0.218
0.0MetXaa: 0.0 ± 0.0
Asn
3.637AsnAla: 3.637 ± 0.509
0.331AsnCys: 0.331 ± 0.139
2.728AsnAsp: 2.728 ± 0.451
3.885AsnGlu: 3.885 ± 0.536
1.571AsnPhe: 1.571 ± 0.255
3.224AsnGly: 3.224 ± 0.641
1.157AsnHis: 1.157 ± 0.262
2.645AsnIle: 2.645 ± 0.493
3.637AsnLys: 3.637 ± 0.518
3.058AsnLeu: 3.058 ± 0.464
1.323AsnMet: 1.323 ± 0.361
1.736AsnAsn: 1.736 ± 0.387
2.48AsnPro: 2.48 ± 0.419
1.736AsnGln: 1.736 ± 0.345
1.818AsnArg: 1.818 ± 0.559
2.728AsnSer: 2.728 ± 0.699
2.893AsnThr: 2.893 ± 0.526
2.48AsnVal: 2.48 ± 0.434
0.744AsnTrp: 0.744 ± 0.279
1.818AsnTyr: 1.818 ± 0.444
0.0AsnXaa: 0.0 ± 0.0
Pro
3.224ProAla: 3.224 ± 0.483
0.413ProCys: 0.413 ± 0.22
2.645ProAsp: 2.645 ± 0.339
3.885ProGlu: 3.885 ± 0.626
1.24ProPhe: 1.24 ± 0.321
0.992ProGly: 0.992 ± 0.317
0.661ProHis: 0.661 ± 0.267
1.405ProIle: 1.405 ± 0.361
2.562ProLys: 2.562 ± 0.489
2.149ProLeu: 2.149 ± 0.373
1.24ProMet: 1.24 ± 0.418
1.901ProAsn: 1.901 ± 0.507
1.075ProPro: 1.075 ± 0.374
1.157ProGln: 1.157 ± 0.265
1.901ProArg: 1.901 ± 0.341
2.149ProSer: 2.149 ± 0.332
2.149ProThr: 2.149 ± 0.398
2.562ProVal: 2.562 ± 0.468
0.661ProTrp: 0.661 ± 0.207
1.323ProTyr: 1.323 ± 0.351
0.0ProXaa: 0.0 ± 0.0
Gln
4.464GlnAla: 4.464 ± 0.509
0.248GlnCys: 0.248 ± 0.131
2.893GlnAsp: 2.893 ± 0.582
2.645GlnGlu: 2.645 ± 0.43
2.149GlnPhe: 2.149 ± 0.357
2.48GlnGly: 2.48 ± 0.582
0.413GlnHis: 0.413 ± 0.194
1.736GlnIle: 1.736 ± 0.393
1.736GlnLys: 1.736 ± 0.431
3.306GlnLeu: 3.306 ± 0.453
0.827GlnMet: 0.827 ± 0.202
0.744GlnAsn: 0.744 ± 0.196
1.323GlnPro: 1.323 ± 0.285
1.653GlnGln: 1.653 ± 0.356
1.736GlnArg: 1.736 ± 0.35
2.397GlnSer: 2.397 ± 0.379
2.066GlnThr: 2.066 ± 0.533
2.48GlnVal: 2.48 ± 0.42
0.744GlnTrp: 0.744 ± 0.21
1.323GlnTyr: 1.323 ± 0.311
0.0GlnXaa: 0.0 ± 0.0
Arg
4.381ArgAla: 4.381 ± 0.534
0.744ArgCys: 0.744 ± 0.254
3.058ArgAsp: 3.058 ± 0.457
3.968ArgGlu: 3.968 ± 0.653
2.149ArgPhe: 2.149 ± 0.444
3.968ArgGly: 3.968 ± 0.481
0.827ArgHis: 0.827 ± 0.278
2.232ArgIle: 2.232 ± 0.453
3.802ArgLys: 3.802 ± 0.649
4.794ArgLeu: 4.794 ± 0.631
1.736ArgMet: 1.736 ± 0.398
3.802ArgAsn: 3.802 ± 0.787
1.818ArgPro: 1.818 ± 0.368
1.488ArgGln: 1.488 ± 0.312
2.48ArgArg: 2.48 ± 0.392
3.058ArgSer: 3.058 ± 0.482
3.389ArgThr: 3.389 ± 0.494
4.133ArgVal: 4.133 ± 0.669
0.331ArgTrp: 0.331 ± 0.169
1.405ArgTyr: 1.405 ± 0.285
0.0ArgXaa: 0.0 ± 0.0
Ser
6.282SerAla: 6.282 ± 1.815
0.661SerCys: 0.661 ± 0.297
4.298SerAsp: 4.298 ± 0.869
2.976SerGlu: 2.976 ± 0.532
2.48SerPhe: 2.48 ± 0.337
5.621SerGly: 5.621 ± 0.674
1.488SerHis: 1.488 ± 0.337
3.306SerIle: 3.306 ± 0.602
3.306SerLys: 3.306 ± 0.471
4.133SerLeu: 4.133 ± 0.592
1.984SerMet: 1.984 ± 0.381
2.645SerAsn: 2.645 ± 0.352
2.397SerPro: 2.397 ± 0.399
2.562SerGln: 2.562 ± 0.458
3.141SerArg: 3.141 ± 0.541
2.397SerSer: 2.397 ± 0.504
2.81SerThr: 2.81 ± 0.482
3.637SerVal: 3.637 ± 0.488
0.744SerTrp: 0.744 ± 0.217
2.81SerTyr: 2.81 ± 0.588
0.0SerXaa: 0.0 ± 0.0
Thr
5.29ThrAla: 5.29 ± 1.062
0.496ThrCys: 0.496 ± 0.238
2.893ThrAsp: 2.893 ± 0.459
3.472ThrGlu: 3.472 ± 0.376
2.149ThrPhe: 2.149 ± 0.346
5.621ThrGly: 5.621 ± 0.686
1.075ThrHis: 1.075 ± 0.256
3.306ThrIle: 3.306 ± 0.505
3.885ThrLys: 3.885 ± 0.463
5.042ThrLeu: 5.042 ± 0.833
1.323ThrMet: 1.323 ± 0.354
1.818ThrAsn: 1.818 ± 0.427
2.893ThrPro: 2.893 ± 0.358
1.818ThrGln: 1.818 ± 0.32
2.562ThrArg: 2.562 ± 0.378
3.72ThrSer: 3.72 ± 0.63
3.554ThrThr: 3.554 ± 0.659
3.72ThrVal: 3.72 ± 0.581
1.157ThrTrp: 1.157 ± 0.471
2.149ThrTyr: 2.149 ± 0.539
0.0ThrXaa: 0.0 ± 0.0
Val
5.951ValAla: 5.951 ± 0.803
0.579ValCys: 0.579 ± 0.248
3.802ValAsp: 3.802 ± 0.519
4.298ValGlu: 4.298 ± 0.55
2.232ValPhe: 2.232 ± 0.599
4.629ValGly: 4.629 ± 0.659
1.157ValHis: 1.157 ± 0.336
3.224ValIle: 3.224 ± 0.494
3.885ValLys: 3.885 ± 0.713
4.794ValLeu: 4.794 ± 0.586
1.984ValMet: 1.984 ± 0.445
3.141ValAsn: 3.141 ± 0.542
2.232ValPro: 2.232 ± 0.405
2.066ValGln: 2.066 ± 0.504
3.885ValArg: 3.885 ± 0.625
3.389ValSer: 3.389 ± 0.552
5.207ValThr: 5.207 ± 0.703
4.381ValVal: 4.381 ± 0.732
0.827ValTrp: 0.827 ± 0.314
1.736ValTyr: 1.736 ± 0.513
0.0ValXaa: 0.0 ± 0.0
Trp
0.579TrpAla: 0.579 ± 0.23
0.331TrpCys: 0.331 ± 0.2
0.331TrpAsp: 0.331 ± 0.156
1.075TrpGlu: 1.075 ± 0.303
0.579TrpPhe: 0.579 ± 0.27
0.827TrpGly: 0.827 ± 0.28
0.331TrpHis: 0.331 ± 0.143
0.661TrpIle: 0.661 ± 0.288
1.405TrpLys: 1.405 ± 0.347
1.488TrpLeu: 1.488 ± 0.388
0.579TrpMet: 0.579 ± 0.174
1.075TrpAsn: 1.075 ± 0.293
0.248TrpPro: 0.248 ± 0.148
0.909TrpGln: 0.909 ± 0.31
1.075TrpArg: 1.075 ± 0.394
1.323TrpSer: 1.323 ± 0.423
0.827TrpThr: 0.827 ± 0.291
1.075TrpVal: 1.075 ± 0.244
0.165TrpTrp: 0.165 ± 0.111
0.248TrpTyr: 0.248 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.645TyrAla: 2.645 ± 0.453
0.248TyrCys: 0.248 ± 0.137
2.645TyrAsp: 2.645 ± 0.555
2.314TyrGlu: 2.314 ± 0.493
0.827TyrPhe: 0.827 ± 0.261
3.224TyrGly: 3.224 ± 0.481
0.331TyrHis: 0.331 ± 0.18
1.405TyrIle: 1.405 ± 0.312
1.323TyrLys: 1.323 ± 0.318
2.232TyrLeu: 2.232 ± 0.443
1.24TyrMet: 1.24 ± 0.276
1.818TyrAsn: 1.818 ± 0.398
0.992TyrPro: 0.992 ± 0.356
1.157TyrGln: 1.157 ± 0.393
2.066TyrArg: 2.066 ± 0.327
1.818TyrSer: 1.818 ± 0.378
3.306TyrThr: 3.306 ± 0.934
2.48TyrVal: 2.48 ± 0.476
0.496TyrTrp: 0.496 ± 0.21
0.992TyrTyr: 0.992 ± 0.276
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (12099 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski