Amino acid dipepetide frequency for Mycobacterium phage Renaud18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.841AlaAla: 12.841 ± 1.291
1.133AlaCys: 1.133 ± 0.248
6.582AlaAsp: 6.582 ± 0.635
8.039AlaGlu: 8.039 ± 0.9
2.482AlaPhe: 2.482 ± 0.38
8.686AlaGly: 8.686 ± 1.169
1.834AlaHis: 1.834 ± 0.292
4.1AlaIle: 4.1 ± 0.523
4.586AlaLys: 4.586 ± 0.526
8.039AlaLeu: 8.039 ± 0.649
2.59AlaMet: 2.59 ± 0.434
3.938AlaAsn: 3.938 ± 0.576
5.179AlaPro: 5.179 ± 0.625
3.669AlaGln: 3.669 ± 0.402
7.122AlaArg: 7.122 ± 0.732
5.341AlaSer: 5.341 ± 0.654
4.964AlaThr: 4.964 ± 0.651
7.122AlaVal: 7.122 ± 0.625
2.428AlaTrp: 2.428 ± 0.361
1.888AlaTyr: 1.888 ± 0.34
0.0AlaXaa: 0.0 ± 0.0
Cys
1.295CysAla: 1.295 ± 0.276
0.162CysCys: 0.162 ± 0.093
0.701CysAsp: 0.701 ± 0.197
0.809CysGlu: 0.809 ± 0.2
0.324CysPhe: 0.324 ± 0.134
1.295CysGly: 1.295 ± 0.306
0.378CysHis: 0.378 ± 0.14
0.432CysIle: 0.432 ± 0.194
0.432CysLys: 0.432 ± 0.162
0.863CysLeu: 0.863 ± 0.284
0.162CysMet: 0.162 ± 0.091
0.593CysAsn: 0.593 ± 0.18
0.809CysPro: 0.809 ± 0.211
0.324CysGln: 0.324 ± 0.133
1.241CysArg: 1.241 ± 0.283
1.187CysSer: 1.187 ± 0.269
0.809CysThr: 0.809 ± 0.233
0.486CysVal: 0.486 ± 0.126
0.216CysTrp: 0.216 ± 0.105
0.27CysTyr: 0.27 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
6.204AspAla: 6.204 ± 0.531
0.971AspCys: 0.971 ± 0.227
4.424AspAsp: 4.424 ± 0.559
4.532AspGlu: 4.532 ± 0.518
1.834AspPhe: 1.834 ± 0.31
6.636AspGly: 6.636 ± 0.634
1.619AspHis: 1.619 ± 0.286
2.644AspIle: 2.644 ± 0.298
1.834AspLys: 1.834 ± 0.298
5.125AspLeu: 5.125 ± 0.534
1.133AspMet: 1.133 ± 0.23
1.403AspAsn: 1.403 ± 0.288
4.64AspPro: 4.64 ± 0.584
2.59AspGln: 2.59 ± 0.422
3.938AspArg: 3.938 ± 0.498
2.913AspSer: 2.913 ± 0.434
3.938AspThr: 3.938 ± 0.439
4.154AspVal: 4.154 ± 0.44
1.565AspTrp: 1.565 ± 0.286
1.834AspTyr: 1.834 ± 0.362
0.0AspXaa: 0.0 ± 0.0
Glu
7.284GluAla: 7.284 ± 0.637
0.971GluCys: 0.971 ± 0.264
3.561GluAsp: 3.561 ± 0.325
3.237GluGlu: 3.237 ± 0.485
2.428GluPhe: 2.428 ± 0.386
2.752GluGly: 2.752 ± 0.44
1.349GluHis: 1.349 ± 0.384
2.428GluIle: 2.428 ± 0.427
2.32GluLys: 2.32 ± 0.366
5.827GluLeu: 5.827 ± 0.584
1.834GluMet: 1.834 ± 0.317
1.726GluAsn: 1.726 ± 0.269
2.859GluPro: 2.859 ± 0.339
3.615GluGln: 3.615 ± 0.511
5.071GluArg: 5.071 ± 0.694
3.075GluSer: 3.075 ± 0.47
3.831GluThr: 3.831 ± 0.49
4.586GluVal: 4.586 ± 0.475
1.673GluTrp: 1.673 ± 0.27
1.457GluTyr: 1.457 ± 0.276
0.0GluXaa: 0.0 ± 0.0
Phe
2.428PheAla: 2.428 ± 0.308
0.486PheCys: 0.486 ± 0.16
2.05PheAsp: 2.05 ± 0.321
2.05PheGlu: 2.05 ± 0.277
0.647PhePhe: 0.647 ± 0.175
2.536PheGly: 2.536 ± 0.457
0.755PheHis: 0.755 ± 0.199
1.295PheIle: 1.295 ± 0.29
0.917PheLys: 0.917 ± 0.251
2.374PheLeu: 2.374 ± 0.38
0.971PheMet: 0.971 ± 0.216
1.133PheAsn: 1.133 ± 0.282
1.457PhePro: 1.457 ± 0.217
0.971PheGln: 0.971 ± 0.252
1.619PheArg: 1.619 ± 0.296
1.133PheSer: 1.133 ± 0.265
2.698PheThr: 2.698 ± 0.417
1.241PheVal: 1.241 ± 0.252
0.162PheTrp: 0.162 ± 0.089
0.701PheTyr: 0.701 ± 0.235
0.0PheXaa: 0.0 ± 0.0
Gly
9.28GlyAla: 9.28 ± 1.404
1.025GlyCys: 1.025 ± 0.241
5.395GlyAsp: 5.395 ± 0.514
4.532GlyGlu: 4.532 ± 0.525
2.644GlyPhe: 2.644 ± 0.327
10.521GlyGly: 10.521 ± 2.751
1.673GlyHis: 1.673 ± 0.277
3.615GlyIle: 3.615 ± 0.528
3.669GlyLys: 3.669 ± 0.445
5.665GlyLeu: 5.665 ± 0.647
1.888GlyMet: 1.888 ± 0.365
3.021GlyAsn: 3.021 ± 0.418
3.831GlyPro: 3.831 ± 0.561
2.967GlyGln: 2.967 ± 0.461
6.312GlyArg: 6.312 ± 0.586
5.071GlySer: 5.071 ± 0.75
6.366GlyThr: 6.366 ± 0.719
6.69GlyVal: 6.69 ± 0.632
2.374GlyTrp: 2.374 ± 0.419
2.698GlyTyr: 2.698 ± 0.34
0.0GlyXaa: 0.0 ± 0.0
His
2.104HisAla: 2.104 ± 0.398
0.432HisCys: 0.432 ± 0.167
1.565HisAsp: 1.565 ± 0.357
0.971HisGlu: 0.971 ± 0.246
0.432HisPhe: 0.432 ± 0.166
2.05HisGly: 2.05 ± 0.328
1.025HisHis: 1.025 ± 0.241
0.971HisIle: 0.971 ± 0.203
0.863HisLys: 0.863 ± 0.212
2.05HisLeu: 2.05 ± 0.395
0.216HisMet: 0.216 ± 0.118
1.133HisAsn: 1.133 ± 0.202
1.511HisPro: 1.511 ± 0.333
0.917HisGln: 0.917 ± 0.261
1.942HisArg: 1.942 ± 0.387
0.863HisSer: 0.863 ± 0.239
1.349HisThr: 1.349 ± 0.404
1.133HisVal: 1.133 ± 0.27
0.54HisTrp: 0.54 ± 0.157
0.755HisTyr: 0.755 ± 0.201
0.0HisXaa: 0.0 ± 0.0
Ile
4.694IleAla: 4.694 ± 0.552
0.54IleCys: 0.54 ± 0.194
3.507IleAsp: 3.507 ± 0.428
2.967IleGlu: 2.967 ± 0.349
0.917IlePhe: 0.917 ± 0.254
3.938IleGly: 3.938 ± 0.453
1.241IleHis: 1.241 ± 0.284
1.187IleIle: 1.187 ± 0.281
1.079IleLys: 1.079 ± 0.269
2.536IleLeu: 2.536 ± 0.416
0.809IleMet: 0.809 ± 0.189
1.942IleAsn: 1.942 ± 0.341
3.237IlePro: 3.237 ± 0.384
1.457IleGln: 1.457 ± 0.233
3.129IleArg: 3.129 ± 0.421
1.942IleSer: 1.942 ± 0.279
3.831IleThr: 3.831 ± 0.588
2.859IleVal: 2.859 ± 0.423
0.863IleTrp: 0.863 ± 0.216
1.025IleTyr: 1.025 ± 0.237
0.0IleXaa: 0.0 ± 0.0
Lys
3.399LysAla: 3.399 ± 0.419
0.647LysCys: 0.647 ± 0.184
2.212LysAsp: 2.212 ± 0.446
1.565LysGlu: 1.565 ± 0.312
1.025LysPhe: 1.025 ± 0.237
2.913LysGly: 2.913 ± 0.368
1.349LysHis: 1.349 ± 0.343
1.511LysIle: 1.511 ± 0.281
1.133LysLys: 1.133 ± 0.268
2.806LysLeu: 2.806 ± 0.477
0.863LysMet: 0.863 ± 0.211
0.701LysAsn: 0.701 ± 0.201
3.129LysPro: 3.129 ± 0.481
1.457LysGln: 1.457 ± 0.267
3.021LysArg: 3.021 ± 0.407
2.05LysSer: 2.05 ± 0.413
2.158LysThr: 2.158 ± 0.369
2.859LysVal: 2.859 ± 0.396
0.755LysTrp: 0.755 ± 0.191
0.755LysTyr: 0.755 ± 0.183
0.0LysXaa: 0.0 ± 0.0
Leu
8.47LeuAla: 8.47 ± 0.659
0.54LeuCys: 0.54 ± 0.208
4.37LeuAsp: 4.37 ± 0.582
4.91LeuGlu: 4.91 ± 0.589
1.511LeuPhe: 1.511 ± 0.264
6.528LeuGly: 6.528 ± 0.61
1.295LeuHis: 1.295 ± 0.24
4.046LeuIle: 4.046 ± 0.507
3.021LeuLys: 3.021 ± 0.387
5.719LeuLeu: 5.719 ± 0.618
1.403LeuMet: 1.403 ± 0.297
2.32LeuAsn: 2.32 ± 0.363
5.341LeuPro: 5.341 ± 0.582
2.428LeuGln: 2.428 ± 0.376
5.935LeuArg: 5.935 ± 0.721
3.885LeuSer: 3.885 ± 0.408
5.125LeuThr: 5.125 ± 0.526
5.557LeuVal: 5.557 ± 0.537
0.971LeuTrp: 0.971 ± 0.255
1.888LeuTyr: 1.888 ± 0.381
0.0LeuXaa: 0.0 ± 0.0
Met
1.834MetAla: 1.834 ± 0.288
0.162MetCys: 0.162 ± 0.09
0.971MetAsp: 0.971 ± 0.182
1.025MetGlu: 1.025 ± 0.227
0.917MetPhe: 0.917 ± 0.204
1.673MetGly: 1.673 ± 0.486
0.162MetHis: 0.162 ± 0.095
0.755MetIle: 0.755 ± 0.172
0.324MetLys: 0.324 ± 0.147
1.133MetLeu: 1.133 ± 0.231
0.593MetMet: 0.593 ± 0.149
1.457MetAsn: 1.457 ± 0.257
0.971MetPro: 0.971 ± 0.268
0.54MetGln: 0.54 ± 0.148
1.834MetArg: 1.834 ± 0.311
2.967MetSer: 2.967 ± 0.416
2.752MetThr: 2.752 ± 0.335
1.619MetVal: 1.619 ± 0.285
0.486MetTrp: 0.486 ± 0.162
0.432MetTyr: 0.432 ± 0.143
0.0MetXaa: 0.0 ± 0.0
Asn
3.561AsnAla: 3.561 ± 0.417
0.054AsnCys: 0.054 ± 0.053
1.619AsnAsp: 1.619 ± 0.321
2.374AsnGlu: 2.374 ± 0.348
0.593AsnPhe: 0.593 ± 0.167
3.777AsnGly: 3.777 ± 0.423
0.701AsnHis: 0.701 ± 0.198
1.565AsnIle: 1.565 ± 0.292
0.971AsnLys: 0.971 ± 0.206
2.644AsnLeu: 2.644 ± 0.39
0.432AsnMet: 0.432 ± 0.145
1.241AsnAsn: 1.241 ± 0.253
2.536AsnPro: 2.536 ± 0.381
1.187AsnGln: 1.187 ± 0.31
2.698AsnArg: 2.698 ± 0.337
1.511AsnSer: 1.511 ± 0.314
2.644AsnThr: 2.644 ± 0.378
2.644AsnVal: 2.644 ± 0.474
0.54AsnTrp: 0.54 ± 0.153
0.701AsnTyr: 0.701 ± 0.188
0.0AsnXaa: 0.0 ± 0.0
Pro
5.773ProAla: 5.773 ± 0.624
0.809ProCys: 0.809 ± 0.214
3.992ProAsp: 3.992 ± 0.462
3.938ProGlu: 3.938 ± 0.601
1.888ProPhe: 1.888 ± 0.326
6.582ProGly: 6.582 ± 0.675
1.726ProHis: 1.726 ± 0.369
2.266ProIle: 2.266 ± 0.423
2.104ProLys: 2.104 ± 0.371
3.237ProLeu: 3.237 ± 0.439
1.133ProMet: 1.133 ± 0.305
2.266ProAsn: 2.266 ± 0.423
3.938ProPro: 3.938 ± 0.532
2.482ProGln: 2.482 ± 0.383
3.561ProArg: 3.561 ± 0.518
3.399ProSer: 3.399 ± 0.382
3.561ProThr: 3.561 ± 0.477
4.532ProVal: 4.532 ± 0.519
1.133ProTrp: 1.133 ± 0.223
1.888ProTyr: 1.888 ± 0.292
0.0ProXaa: 0.0 ± 0.0
Gln
4.37GlnAla: 4.37 ± 0.39
0.432GlnCys: 0.432 ± 0.138
1.942GlnAsp: 1.942 ± 0.324
1.295GlnGlu: 1.295 ± 0.378
1.241GlnPhe: 1.241 ± 0.258
2.967GlnGly: 2.967 ± 0.494
0.809GlnHis: 0.809 ± 0.185
2.104GlnIle: 2.104 ± 0.333
1.78GlnLys: 1.78 ± 0.305
3.453GlnLeu: 3.453 ± 0.498
0.971GlnMet: 0.971 ± 0.201
0.647GlnAsn: 0.647 ± 0.242
2.967GlnPro: 2.967 ± 0.447
1.511GlnGln: 1.511 ± 0.334
2.644GlnArg: 2.644 ± 0.497
1.565GlnSer: 1.565 ± 0.238
1.996GlnThr: 1.996 ± 0.285
2.428GlnVal: 2.428 ± 0.289
0.378GlnTrp: 0.378 ± 0.188
1.133GlnTyr: 1.133 ± 0.245
0.0GlnXaa: 0.0 ± 0.0
Arg
6.474ArgAla: 6.474 ± 0.685
1.403ArgCys: 1.403 ± 0.338
4.208ArgAsp: 4.208 ± 0.585
4.316ArgGlu: 4.316 ± 0.603
2.32ArgPhe: 2.32 ± 0.348
4.586ArgGly: 4.586 ± 0.425
1.942ArgHis: 1.942 ± 0.393
3.723ArgIle: 3.723 ± 0.497
3.183ArgLys: 3.183 ± 0.508
5.935ArgLeu: 5.935 ± 0.672
2.482ArgMet: 2.482 ± 0.469
2.482ArgAsn: 2.482 ± 0.402
4.1ArgPro: 4.1 ± 0.509
2.482ArgGln: 2.482 ± 0.463
5.071ArgArg: 5.071 ± 0.722
3.938ArgSer: 3.938 ± 0.437
3.723ArgThr: 3.723 ± 0.514
5.018ArgVal: 5.018 ± 0.6
1.673ArgTrp: 1.673 ± 0.281
2.212ArgTyr: 2.212 ± 0.339
0.0ArgXaa: 0.0 ± 0.0
Ser
5.125SerAla: 5.125 ± 0.527
0.378SerCys: 0.378 ± 0.151
4.316SerAsp: 4.316 ± 0.478
3.561SerGlu: 3.561 ± 0.443
1.673SerPhe: 1.673 ± 0.264
6.258SerGly: 6.258 ± 1.038
0.971SerHis: 0.971 ± 0.207
2.266SerIle: 2.266 ± 0.275
2.266SerLys: 2.266 ± 0.378
3.885SerLeu: 3.885 ± 0.379
1.726SerMet: 1.726 ± 0.333
1.834SerAsn: 1.834 ± 0.289
2.806SerPro: 2.806 ± 0.424
1.78SerGln: 1.78 ± 0.275
2.967SerArg: 2.967 ± 0.438
3.021SerSer: 3.021 ± 0.539
2.967SerThr: 2.967 ± 0.361
3.777SerVal: 3.777 ± 0.444
1.349SerTrp: 1.349 ± 0.246
1.241SerTyr: 1.241 ± 0.282
0.0SerXaa: 0.0 ± 0.0
Thr
6.204ThrAla: 6.204 ± 0.65
0.755ThrCys: 0.755 ± 0.213
3.992ThrAsp: 3.992 ± 0.422
4.046ThrGlu: 4.046 ± 0.439
1.726ThrPhe: 1.726 ± 0.248
6.636ThrGly: 6.636 ± 0.8
1.349ThrHis: 1.349 ± 0.256
3.399ThrIle: 3.399 ± 0.478
1.996ThrLys: 1.996 ± 0.339
4.262ThrLeu: 4.262 ± 0.509
1.187ThrMet: 1.187 ± 0.257
2.374ThrAsn: 2.374 ± 0.31
4.964ThrPro: 4.964 ± 0.485
2.266ThrGln: 2.266 ± 0.44
4.37ThrArg: 4.37 ± 0.562
3.669ThrSer: 3.669 ± 0.458
4.694ThrThr: 4.694 ± 0.582
5.773ThrVal: 5.773 ± 0.577
1.349ThrTrp: 1.349 ± 0.27
1.619ThrTyr: 1.619 ± 0.303
0.0ThrXaa: 0.0 ± 0.0
Val
7.337ValAla: 7.337 ± 0.664
1.187ValCys: 1.187 ± 0.261
4.964ValAsp: 4.964 ± 0.453
4.964ValGlu: 4.964 ± 0.537
1.726ValPhe: 1.726 ± 0.272
5.611ValGly: 5.611 ± 0.499
1.565ValHis: 1.565 ± 0.328
3.561ValIle: 3.561 ± 0.397
2.05ValLys: 2.05 ± 0.313
5.827ValLeu: 5.827 ± 0.511
1.403ValMet: 1.403 ± 0.307
2.644ValAsn: 2.644 ± 0.367
3.453ValPro: 3.453 ± 0.354
2.212ValGln: 2.212 ± 0.321
4.586ValArg: 4.586 ± 0.623
4.316ValSer: 4.316 ± 0.824
5.665ValThr: 5.665 ± 0.527
5.341ValVal: 5.341 ± 0.591
1.511ValTrp: 1.511 ± 0.254
1.403ValTyr: 1.403 ± 0.262
0.0ValXaa: 0.0 ± 0.0
Trp
1.726TrpAla: 1.726 ± 0.333
0.378TrpCys: 0.378 ± 0.142
1.619TrpAsp: 1.619 ± 0.264
0.755TrpGlu: 0.755 ± 0.202
0.593TrpPhe: 0.593 ± 0.164
1.133TrpGly: 1.133 ± 0.259
0.324TrpHis: 0.324 ± 0.132
0.755TrpIle: 0.755 ± 0.197
1.133TrpLys: 1.133 ± 0.202
1.834TrpLeu: 1.834 ± 0.31
0.432TrpMet: 0.432 ± 0.176
0.486TrpAsn: 0.486 ± 0.15
1.511TrpPro: 1.511 ± 0.25
1.025TrpGln: 1.025 ± 0.279
1.834TrpArg: 1.834 ± 0.391
0.863TrpSer: 0.863 ± 0.206
1.78TrpThr: 1.78 ± 0.38
1.726TrpVal: 1.726 ± 0.343
0.863TrpTrp: 0.863 ± 0.228
0.701TrpTyr: 0.701 ± 0.176
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.212TyrAla: 2.212 ± 0.305
0.324TyrCys: 0.324 ± 0.137
1.942TyrAsp: 1.942 ± 0.295
1.996TyrGlu: 1.996 ± 0.369
0.701TyrPhe: 0.701 ± 0.237
2.158TyrGly: 2.158 ± 0.434
0.755TyrHis: 0.755 ± 0.186
1.079TyrIle: 1.079 ± 0.254
0.647TyrLys: 0.647 ± 0.175
2.05TyrLeu: 2.05 ± 0.345
0.324TyrMet: 0.324 ± 0.116
0.647TyrAsn: 0.647 ± 0.177
0.971TyrPro: 0.971 ± 0.269
0.755TyrGln: 0.755 ± 0.173
2.374TyrArg: 2.374 ± 0.357
1.457TyrSer: 1.457 ± 0.246
1.78TyrThr: 1.78 ± 0.266
1.78TyrVal: 1.78 ± 0.339
0.647TyrTrp: 0.647 ± 0.182
0.971TyrTyr: 0.971 ± 0.245
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 112 proteins (18536 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski