Tieghemostelium lacteum

Taxonomy: cellular organisms; Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales; Raperosteliaceae; Tieghemostelium

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10208 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A152A2X9|A0A152A2X9_9MYCE ABC transporter D family protein OS=Tieghemostelium lacteum OX=361077 GN=DLAC_02605 PE=4 SV=1
MM1 pKa = 7.47YY2 pKa = 8.42STKK5 pKa = 10.71LFVLFNIFILSLALVNAEE23 pKa = 3.78IYY25 pKa = 10.26GLKK28 pKa = 9.84SQQEE32 pKa = 4.28VHH34 pKa = 7.12DD35 pKa = 5.27SISVINVGDD44 pKa = 3.4NDD46 pKa = 3.71VRR48 pKa = 11.84YY49 pKa = 8.17LTYY52 pKa = 10.36FSSLGDD58 pKa = 3.63FYY60 pKa = 11.83VSGVTANVDD69 pKa = 3.56STGQNADD76 pKa = 3.1VTTSAFYY83 pKa = 10.22FSSGTSSILNLPLSYY98 pKa = 10.43YY99 pKa = 10.33YY100 pKa = 10.72SYY102 pKa = 10.7ATASVSPGTVNAQGSVAAWAPSAIIEE128 pKa = 4.22YY129 pKa = 10.44EE130 pKa = 4.16EE131 pKa = 4.38NNGQAGFQLNSSDD144 pKa = 5.67KK145 pKa = 8.76VLGWIRR151 pKa = 11.84LDD153 pKa = 3.38LYY155 pKa = 11.53GNFDD159 pKa = 4.32IDD161 pKa = 4.31STDD164 pKa = 3.29QTITATANGNSISTKK179 pKa = 10.08VYY181 pKa = 9.98TIDD184 pKa = 3.64AVSPDD189 pKa = 3.96GIFALRR195 pKa = 11.84FVVSGAPVAIGEE207 pKa = 4.35DD208 pKa = 3.67VEE210 pKa = 4.54ITAEE214 pKa = 3.85QSKK217 pKa = 11.22ADD219 pKa = 3.75VVINDD224 pKa = 4.04YY225 pKa = 11.3YY226 pKa = 11.35DD227 pKa = 4.11SNVNQNSIGCDD238 pKa = 2.97ASEE241 pKa = 4.7PFLSCRR247 pKa = 11.84STGPSSNTNSRR258 pKa = 11.84LALASFFLAASFEE271 pKa = 4.17VDD273 pKa = 3.76ANIDD277 pKa = 3.51NDD279 pKa = 4.17AVSVDD284 pKa = 3.63AGDD287 pKa = 3.94VEE289 pKa = 5.99IGFNWVKK296 pKa = 9.61TVSVNNGQGSANVVAEE312 pKa = 4.8TYY314 pKa = 10.43ASKK317 pKa = 10.89DD318 pKa = 3.23GNSFINAKK326 pKa = 10.46FNGTYY331 pKa = 9.79SGQLIVHH338 pKa = 6.45SFDD341 pKa = 3.08TVRR344 pKa = 11.84PDD346 pKa = 3.17TVTWDD351 pKa = 3.54PTVGAGISSSSSAAVLKK368 pKa = 10.75VSAFMLFGLVLTLFII383 pKa = 5.56

Molecular weight: 40.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A151ZEN2|A0A151ZEN2_9MYCE Trafficking protein particle complex subunit 2-like protein OS=Tieghemostelium lacteum OX=361077 GN=DLAC_06317 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84LFQQAQNNINNNNNHH17 pKa = 6.96RR18 pKa = 11.84DD19 pKa = 3.61RR20 pKa = 11.84EE21 pKa = 4.4PIPNRR26 pKa = 11.84HH27 pKa = 5.31SRR29 pKa = 11.84SRR31 pKa = 11.84SRR33 pKa = 11.84SRR35 pKa = 11.84SRR37 pKa = 11.84SRR39 pKa = 11.84SRR41 pKa = 11.84SRR43 pKa = 11.84SRR45 pKa = 11.84SRR47 pKa = 11.84SRR49 pKa = 11.84SRR51 pKa = 11.84SRR53 pKa = 11.84SRR55 pKa = 11.84SRR57 pKa = 11.84SRR59 pKa = 11.84SRR61 pKa = 11.84SRR63 pKa = 11.84SRR65 pKa = 11.84SRR67 pKa = 11.84SRR69 pKa = 11.84SSFLINRR76 pKa = 11.84KK77 pKa = 7.37TKK79 pKa = 10.41

Molecular weight: 9.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10208

0

10208

5820820

66

9353

570.2

64.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.539 ± 0.019

1.418 ± 0.011

5.366 ± 0.014

5.743 ± 0.025

4.574 ± 0.019

4.48 ± 0.023

2.066 ± 0.012

7.965 ± 0.022

7.309 ± 0.026

9.467 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.889 ± 0.008

7.694 ± 0.029

4.378 ± 0.019

5.203 ± 0.027

3.098 ± 0.014

9.557 ± 0.03

6.383 ± 0.026

5.093 ± 0.017

0.822 ± 0.007

3.956 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski