Roseomonas nepalensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Roseomonas

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5879 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A502FI93|A0A502FI93_9PROT 50S ribosomal protein L29 OS=Roseomonas nepalensis OX=1854500 GN=rpmC PE=3 SV=1
MM1 pKa = 7.37TYY3 pKa = 10.71VVTEE7 pKa = 3.8NCIKK11 pKa = 10.19CKK13 pKa = 10.76YY14 pKa = 7.69MDD16 pKa = 4.28CVEE19 pKa = 4.32VCPVDD24 pKa = 3.73CFYY27 pKa = 11.62VGANMLVIHH36 pKa = 7.23PDD38 pKa = 2.97EE39 pKa = 5.88CIDD42 pKa = 4.44CGVCEE47 pKa = 4.44PEE49 pKa = 4.82CPAEE53 pKa = 5.06AILPDD58 pKa = 3.79SDD60 pKa = 5.87DD61 pKa = 4.36RR62 pKa = 11.84ATDD65 pKa = 3.23WAEE68 pKa = 3.82LNRR71 pKa = 11.84TYY73 pKa = 10.87SAQWPNISRR82 pKa = 11.84KK83 pKa = 10.17GEE85 pKa = 3.85PPADD89 pKa = 3.42AKK91 pKa = 10.96EE92 pKa = 4.03WDD94 pKa = 3.97GKK96 pKa = 9.54PGKK99 pKa = 10.47AEE101 pKa = 4.42LFDD104 pKa = 4.15AAPGEE109 pKa = 4.58GG110 pKa = 3.46

Molecular weight: 12.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A502FCT5|A0A502FCT5_9PROT Curli production assembly/transport component CsgF OS=Roseomonas nepalensis OX=1854500 GN=EAH89_23700 PE=4 SV=1
MM1 pKa = 7.72ASRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84AGGSAGMPRR15 pKa = 11.84AARR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84SRR22 pKa = 11.84ASASAGSRR30 pKa = 11.84SASSRR35 pKa = 11.84ASGRR39 pKa = 11.84SQRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84SSRR47 pKa = 11.84LAAWARR53 pKa = 11.84ARR55 pKa = 11.84QAASGPSRR63 pKa = 11.84AQVAARR69 pKa = 11.84KK70 pKa = 8.58AAKK73 pKa = 9.39EE74 pKa = 3.82RR75 pKa = 11.84ASASRR80 pKa = 11.84PGFWAGGSWSWRR92 pKa = 11.84TRR94 pKa = 3.11

Molecular weight: 10.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5879

0

5879

1855697

25

2451

315.6

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.219 ± 0.061

0.78 ± 0.009

5.0 ± 0.022

5.705 ± 0.03

3.121 ± 0.019

9.594 ± 0.031

1.945 ± 0.015

3.664 ± 0.022

1.777 ± 0.019

10.967 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.201 ± 0.014

1.994 ± 0.018

6.402 ± 0.029

2.647 ± 0.021

8.836 ± 0.037

4.578 ± 0.022

4.916 ± 0.027

7.491 ± 0.027

1.432 ± 0.015

1.726 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski