Bat coronavirus 512/2005 (BtCoV) (BtCoV/512/2005)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Pedacovirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q0Q466|SPIKE_BC512 Spike glycoprotein OS=Bat coronavirus 512/2005 OX=693999 GN=S PE=3 SV=1
MM1 pKa = 7.48FLGLFQYY8 pKa = 10.55TIDD11 pKa = 3.56TAVEE15 pKa = 4.13HH16 pKa = 5.72TVEE19 pKa = 4.19HH20 pKa = 6.71ANLSQEE26 pKa = 4.13EE27 pKa = 4.01ALMLEE32 pKa = 4.53EE33 pKa = 5.03NIVPLRR39 pKa = 11.84QATHH43 pKa = 3.98VTGFLLTSVFVYY55 pKa = 8.62FFALFKK61 pKa = 10.8ASSYY65 pKa = 10.53KK66 pKa = 10.47RR67 pKa = 11.84NLLLFLARR75 pKa = 11.84LLALLIYY82 pKa = 10.55APILIFCGAYY92 pKa = 9.67LDD94 pKa = 4.08AFIVVATLTSRR105 pKa = 11.84LLFLTYY111 pKa = 10.13YY112 pKa = 8.13SWRR115 pKa = 11.84YY116 pKa = 7.08KK117 pKa = 9.3TYY119 pKa = 10.84KK120 pKa = 10.02FLIYY124 pKa = 10.5NSSTLMFLHH133 pKa = 6.49GHH135 pKa = 6.01ANYY138 pKa = 10.72YY139 pKa = 9.83NGRR142 pKa = 11.84PYY144 pKa = 11.59VMLEE148 pKa = 4.08GGSHH152 pKa = 5.67YY153 pKa = 10.09VTLGTDD159 pKa = 3.41IVPFVSRR166 pKa = 11.84SNLYY170 pKa = 9.95LAIRR174 pKa = 11.84GSAEE178 pKa = 3.87SDD180 pKa = 2.98IQLLRR185 pKa = 11.84TVEE188 pKa = 4.15LLDD191 pKa = 3.99GNYY194 pKa = 10.59LYY196 pKa = 10.24IFSSCQVVGVTNSGFEE212 pKa = 4.14EE213 pKa = 4.15IQLDD217 pKa = 4.2EE218 pKa = 4.28YY219 pKa = 11.22ATISEE224 pKa = 4.39

Molecular weight: 25.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q0Q463|VME1_BC512 Membrane protein OS=Bat coronavirus 512/2005 OX=693999 GN=M PE=3 SV=1
MM1 pKa = 7.67ASVKK5 pKa = 9.27FQPRR9 pKa = 11.84GRR11 pKa = 11.84SKK13 pKa = 11.13GRR15 pKa = 11.84VPLSLFAPLRR25 pKa = 11.84VTDD28 pKa = 4.44EE29 pKa = 4.06KK30 pKa = 10.97PLYY33 pKa = 10.13KK34 pKa = 10.28VLPNNAVPQGMGGKK48 pKa = 9.05DD49 pKa = 3.18QQIGYY54 pKa = 8.72WVEE57 pKa = 3.76QQRR60 pKa = 11.84WRR62 pKa = 11.84MRR64 pKa = 11.84RR65 pKa = 11.84GDD67 pKa = 4.01RR68 pKa = 11.84VDD70 pKa = 5.53LPSNWHH76 pKa = 6.43FYY78 pKa = 11.01FLGTGPHH85 pKa = 6.48SDD87 pKa = 3.13LPFRR91 pKa = 11.84KK92 pKa = 8.78RR93 pKa = 11.84TDD95 pKa = 3.05GVFWVAIDD103 pKa = 3.88GAKK106 pKa = 8.22TQPTGLGVRR115 pKa = 11.84KK116 pKa = 10.04SSEE119 pKa = 3.92KK120 pKa = 10.41PLVPKK125 pKa = 10.46FKK127 pKa = 11.02NKK129 pKa = 10.07LPNNVEE135 pKa = 3.87IVEE138 pKa = 4.27PTTPNNSRR146 pKa = 11.84ANSRR150 pKa = 11.84SRR152 pKa = 11.84SRR154 pKa = 11.84GGQSNSRR161 pKa = 11.84GNSQNRR167 pKa = 11.84GDD169 pKa = 3.61KK170 pKa = 10.65SRR172 pKa = 11.84NQSRR176 pKa = 11.84NRR178 pKa = 11.84SQSNDD183 pKa = 3.28RR184 pKa = 11.84GSDD187 pKa = 3.4SRR189 pKa = 11.84DD190 pKa = 3.43DD191 pKa = 3.64LVAAVKK197 pKa = 10.31KK198 pKa = 10.49ALEE201 pKa = 4.24DD202 pKa = 3.91LGVGAAKK209 pKa = 10.36PKK211 pKa = 10.86GKK213 pKa = 7.85TQSGKK218 pKa = 8.26NTPKK222 pKa = 10.31NKK224 pKa = 9.62SRR226 pKa = 11.84SGSVQRR232 pKa = 11.84AEE234 pKa = 5.3AKK236 pKa = 10.11DD237 pKa = 3.43KK238 pKa = 10.96PEE240 pKa = 3.49WRR242 pKa = 11.84RR243 pKa = 11.84TPSGDD248 pKa = 3.18EE249 pKa = 3.96SVEE252 pKa = 4.08VCFGPRR258 pKa = 11.84GGTRR262 pKa = 11.84NFGSSEE268 pKa = 3.91FVAKK272 pKa = 10.21GVNAPGYY279 pKa = 8.95AQAASLVPGAAALLFGGNVATKK301 pKa = 10.21EE302 pKa = 4.0MADD305 pKa = 3.62GVEE308 pKa = 3.54ITYY311 pKa = 8.85TYY313 pKa = 11.13KK314 pKa = 10.29MLVPKK319 pKa = 10.12DD320 pKa = 4.0DD321 pKa = 4.07KK322 pKa = 11.38NLEE325 pKa = 3.86IFLAQVDD332 pKa = 4.47AYY334 pKa = 10.16KK335 pKa = 10.93LGDD338 pKa = 3.84PKK340 pKa = 10.54PQRR343 pKa = 11.84KK344 pKa = 8.55VKK346 pKa = 10.45RR347 pKa = 11.84SRR349 pKa = 11.84TPTPKK354 pKa = 10.02PATEE358 pKa = 3.82PVYY361 pKa = 11.2DD362 pKa = 5.21DD363 pKa = 3.74VAADD367 pKa = 3.41PTYY370 pKa = 11.66ANLEE374 pKa = 3.87WDD376 pKa = 3.74TTVEE380 pKa = 4.85DD381 pKa = 4.1GVEE384 pKa = 4.22MINEE388 pKa = 4.04VFDD391 pKa = 3.88TQNN394 pKa = 2.67

Molecular weight: 43.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

13213

76

6793

1887.6

209.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.259 ± 0.191

3.277 ± 0.401

5.752 ± 0.545

3.837 ± 0.155

5.601 ± 0.157

6.751 ± 0.229

1.862 ± 0.157

4.859 ± 0.185

5.775 ± 0.595

9.362 ± 0.644

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.937 ± 0.109

5.661 ± 0.359

3.64 ± 0.238

3.035 ± 0.307

3.459 ± 0.25

7.144 ± 0.169

6.229 ± 0.42

10.073 ± 0.215

1.143 ± 0.207

4.344 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski