Grapevine vein clearing virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Badnavirus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8TPA8|F8TPA8_9VIRU Uncharacterized protein OS=Grapevine vein clearing virus OX=1050407 PE=4 SV=1
MM1 pKa = 7.51QSIEE5 pKa = 4.04QQQFEE10 pKa = 4.57AEE12 pKa = 3.96IEE14 pKa = 3.92SWEE17 pKa = 4.07RR18 pKa = 11.84SEE20 pKa = 4.03RR21 pKa = 11.84TPLHH25 pKa = 6.78GYY27 pKa = 9.71RR28 pKa = 11.84DD29 pKa = 3.63LVEE32 pKa = 4.0YY33 pKa = 9.79PRR35 pKa = 11.84YY36 pKa = 9.69EE37 pKa = 4.2RR38 pKa = 11.84NQHH41 pKa = 5.38FPSAKK46 pKa = 8.29FPCYY50 pKa = 10.25HH51 pKa = 6.46FVAEE55 pKa = 4.62KK56 pKa = 11.07DD57 pKa = 3.88NVHH60 pKa = 5.63ATYY63 pKa = 10.08TKK65 pKa = 10.7GDD67 pKa = 4.73RR68 pKa = 11.84IPQLLNTLYY77 pKa = 10.7DD78 pKa = 3.72LQVNQCHH85 pKa = 5.34NQAVIYY91 pKa = 10.46DD92 pKa = 4.59RR93 pKa = 11.84IQLLSRR99 pKa = 11.84YY100 pKa = 6.67TVRR103 pKa = 11.84KK104 pKa = 9.27GKK106 pKa = 9.01PLPAIPEE113 pKa = 3.99EE114 pKa = 4.51SVLKK118 pKa = 10.49EE119 pKa = 4.0PEE121 pKa = 3.99EE122 pKa = 4.37SSTEE126 pKa = 3.86LKK128 pKa = 10.51HH129 pKa = 7.36QIEE132 pKa = 4.77LLRR135 pKa = 11.84ADD137 pKa = 3.85LRR139 pKa = 11.84EE140 pKa = 3.9IKK142 pKa = 10.56ANQSSLRR149 pKa = 11.84LAISEE154 pKa = 3.86IRR156 pKa = 11.84EE157 pKa = 4.35SITDD161 pKa = 3.67LTARR165 pKa = 11.84EE166 pKa = 4.27SAPKK170 pKa = 9.86PIEE173 pKa = 4.39AEE175 pKa = 4.06TAYY178 pKa = 9.53LTAQLKK184 pKa = 9.62VQVQEE189 pKa = 3.9IKK191 pKa = 9.77TALTEE196 pKa = 3.92IKK198 pKa = 9.56TFARR202 pKa = 11.84TLVPEE207 pKa = 4.46RR208 pKa = 4.22

Molecular weight: 24.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8TPA9|F8TPA9_9VIRU Reverse transcriptase OS=Grapevine vein clearing virus OX=1050407 PE=4 SV=2
MM1 pKa = 7.02STWQIAAAAEE11 pKa = 4.31EE12 pKa = 4.38YY13 pKa = 10.94KK14 pKa = 11.01NAIKK18 pKa = 9.56ATATLTKK25 pKa = 10.32DD26 pKa = 2.95EE27 pKa = 4.37RR28 pKa = 11.84AVGFVKK34 pKa = 10.35PHH36 pKa = 5.19EE37 pKa = 4.38FEE39 pKa = 5.42PNFSDD44 pKa = 5.0TNIQRR49 pKa = 11.84QNNTLIHH56 pKa = 6.6LLIQSLEE63 pKa = 4.2EE64 pKa = 4.01IKK66 pKa = 10.55EE67 pKa = 3.81LRR69 pKa = 11.84AQVQTLNDD77 pKa = 4.19RR78 pKa = 11.84IITLEE83 pKa = 3.92KK84 pKa = 10.39GKK86 pKa = 10.86APVTLPDD93 pKa = 3.54NVVEE97 pKa = 4.78QISTQLKK104 pKa = 7.93EE105 pKa = 4.4AKK107 pKa = 9.71FGQPKK112 pKa = 9.87EE113 pKa = 4.06GLVKK117 pKa = 9.62GTKK120 pKa = 8.65GTFRR124 pKa = 11.84VWKK127 pKa = 10.13

Molecular weight: 14.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2276

127

1941

758.7

86.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.854 ± 0.608

1.538 ± 0.484

5.141 ± 0.901

7.645 ± 1.671

3.032 ± 0.229

5.8 ± 1.561

2.417 ± 0.197

6.634 ± 0.126

6.591 ± 0.808

8.172 ± 0.691

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.774

3.515 ± 0.439

5.624 ± 0.391

5.844 ± 0.639

6.195 ± 0.56

6.591 ± 0.888

6.151 ± 0.897

5.316 ± 0.226

1.406 ± 0.289

3.339 ± 0.564
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski