Marinifilum breve

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Marinifilaceae; Marinifilum

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4572 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V3ZWW5|A0A2V3ZWW5_9BACT Quinolinate synthase OS=Marinifilum breve OX=2184082 GN=DF185_16900 PE=4 SV=1
MM1 pKa = 7.93IDD3 pKa = 3.46LLKK6 pKa = 10.3TKK8 pKa = 9.59TRR10 pKa = 11.84LLLIIAIIFGMGTLTSCEE28 pKa = 4.07EE29 pKa = 4.0EE30 pKa = 4.11GAKK33 pKa = 10.88YY34 pKa = 10.14IDD36 pKa = 4.93DD37 pKa = 5.13LDD39 pKa = 4.49TIITRR44 pKa = 11.84YY45 pKa = 10.23DD46 pKa = 3.27DD47 pKa = 4.45SFDD50 pKa = 3.56FSTVKK55 pKa = 10.04TYY57 pKa = 11.29VMPDD61 pKa = 3.05TVVYY65 pKa = 10.13VPEE68 pKa = 3.94VSIDD72 pKa = 3.47SEE74 pKa = 4.02KK75 pKa = 10.7RR76 pKa = 11.84AEE78 pKa = 3.85FDD80 pKa = 3.81AAVLKK85 pKa = 10.64QLADD89 pKa = 3.24NFEE92 pKa = 4.28RR93 pKa = 11.84LGYY96 pKa = 8.73TRR98 pKa = 11.84LAEE101 pKa = 4.69GDD103 pKa = 4.08DD104 pKa = 4.0SVDD107 pKa = 3.14PDD109 pKa = 4.28VIVTATLIEE118 pKa = 4.36NDD120 pKa = 3.13VHH122 pKa = 7.83AVYY125 pKa = 9.24PYY127 pKa = 9.42PWYY130 pKa = 10.08DD131 pKa = 2.96YY132 pKa = 9.91WYY134 pKa = 9.11WFDD137 pKa = 3.32WPFWGLPDD145 pKa = 5.12IGPGWYY151 pKa = 8.6PQYY154 pKa = 10.05PPYY157 pKa = 9.73WGVYY161 pKa = 9.5SYY163 pKa = 7.6TTGTLVVDD171 pKa = 3.58MMQPVSIEE179 pKa = 3.64QDD181 pKa = 3.05KK182 pKa = 10.78QIPVVWNGIFNGIASIGLSDD202 pKa = 4.9RR203 pKa = 11.84MLDD206 pKa = 4.75GIDD209 pKa = 3.55QMFEE213 pKa = 3.59QSPYY217 pKa = 10.83LSQEE221 pKa = 3.62

Molecular weight: 25.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V3ZSW5|A0A2V3ZSW5_9BACT Uncharacterized protein OS=Marinifilum breve OX=2184082 GN=DF185_20055 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 8.89RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.89HH16 pKa = 3.92GFRR19 pKa = 11.84EE20 pKa = 4.21RR21 pKa = 11.84MSSVSGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.11GRR39 pKa = 11.84KK40 pKa = 8.0KK41 pKa = 10.62VSVSSEE47 pKa = 3.8RR48 pKa = 11.84RR49 pKa = 11.84HH50 pKa = 5.76KK51 pKa = 10.9AA52 pKa = 2.86

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4572

0

4572

1604438

12

3198

350.9

39.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.113 ± 0.039

0.956 ± 0.012

5.653 ± 0.023

7.094 ± 0.036

5.022 ± 0.028

6.35 ± 0.037

1.818 ± 0.017

7.722 ± 0.032

8.07 ± 0.041

9.281 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.017

6.017 ± 0.032

3.246 ± 0.019

3.367 ± 0.022

3.682 ± 0.024

6.663 ± 0.03

4.995 ± 0.031

6.209 ± 0.031

1.109 ± 0.012

4.201 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski