Danionella translucida

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi; Otophysi; Cypriniphysae; Cypriniformes;

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32815 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A553RGW2|A0A553RGW2_9TELE Uncharacterized protein OS=Danionella translucida OX=623744 GN=DNTS_018429 PE=4 SV=1
MM1 pKa = 7.62CRR3 pKa = 11.84PCRR6 pKa = 11.84LVPTLGQLHH15 pKa = 7.14DD16 pKa = 4.14LRR18 pKa = 11.84LVNGFNSSCFGRR30 pKa = 11.84VEE32 pKa = 4.22INLNGNWGTVCDD44 pKa = 4.07THH46 pKa = 7.24WDD48 pKa = 3.54LKK50 pKa = 10.72DD51 pKa = 3.22AHH53 pKa = 6.4VVCKK57 pKa = 10.22QLQCGLAIIAHH68 pKa = 6.35LNGIFGEE75 pKa = 4.9GSRR78 pKa = 11.84VWLDD82 pKa = 3.23QTHH85 pKa = 5.82CTGSEE90 pKa = 3.88GHH92 pKa = 6.91LVNCSQRR99 pKa = 11.84TQLGHH104 pKa = 6.74CEE106 pKa = 4.08DD107 pKa = 4.82GEE109 pKa = 4.44DD110 pKa = 4.47AGVICSGPSMFQEE123 pKa = 4.91SSNEE127 pKa = 3.83DD128 pKa = 3.69DD129 pKa = 5.55DD130 pKa = 5.45DD131 pKa = 6.45ADD133 pKa = 4.11YY134 pKa = 11.7EE135 pKa = 4.72HH136 pKa = 7.33VDD138 pKa = 3.35HH139 pKa = 7.67DD140 pKa = 4.09LANYY144 pKa = 10.01EE145 pKa = 4.72EE146 pKa = 4.91INSSGSEE153 pKa = 3.83DD154 pKa = 4.03DD155 pKa = 4.71YY156 pKa = 12.19VNIDD160 pKa = 4.73NDD162 pKa = 4.1TEE164 pKa = 4.08DD165 pKa = 3.48QKK167 pKa = 11.22MKK169 pKa = 10.62NSEE172 pKa = 4.94DD173 pKa = 3.46IDD175 pKa = 4.35SSGSEE180 pKa = 3.95DD181 pKa = 5.02DD182 pKa = 4.45YY183 pKa = 12.23VNANNDD189 pKa = 3.56TEE191 pKa = 4.12DD192 pKa = 3.4QKK194 pKa = 11.31MKK196 pKa = 10.59DD197 pKa = 3.63SEE199 pKa = 5.49DD200 pKa = 3.46IDD202 pKa = 4.32SSGSEE207 pKa = 3.75DD208 pKa = 5.12DD209 pKa = 4.03YY210 pKa = 12.03VNVNNDD216 pKa = 3.25TEE218 pKa = 4.14DD219 pKa = 3.41QKK221 pKa = 11.21MKK223 pKa = 10.66NSEE226 pKa = 4.72DD227 pKa = 3.39IDD229 pKa = 4.29LSGSEE234 pKa = 4.11VDD236 pKa = 5.21CVNVDD241 pKa = 3.72NYY243 pKa = 11.59TNDD246 pKa = 3.63NDD248 pKa = 4.32DD249 pKa = 4.37HH250 pKa = 5.95MLKK253 pKa = 10.65NSGDD257 pKa = 3.62TDD259 pKa = 3.97SEE261 pKa = 3.81EE262 pKa = 6.11DD263 pKa = 3.64YY264 pKa = 11.78VNWKK268 pKa = 9.61VCEE271 pKa = 4.0TDD273 pKa = 4.64LEE275 pKa = 4.38NLL277 pKa = 3.67

Molecular weight: 30.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A553QVK6|A0A553QVK6_9TELE Uncharacterized protein (Fragment) OS=Danionella translucida OX=623744 GN=DNTS_032826 PE=4 SV=1
MM1 pKa = 7.5LNLIRR6 pKa = 11.84VLLQLPLHH14 pKa = 5.9GRR16 pKa = 11.84KK17 pKa = 9.18SRR19 pKa = 11.84ITPRR23 pKa = 11.84VLKK26 pKa = 10.3LRR28 pKa = 11.84KK29 pKa = 9.11RR30 pKa = 11.84RR31 pKa = 11.84KK32 pKa = 9.92ASIKK36 pKa = 9.13WPSVV40 pKa = 2.94

Molecular weight: 4.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23793

9022

32815

20250203

8

27949

617.1

68.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.315 ± 0.012

2.242 ± 0.015

5.132 ± 0.009

7.198 ± 0.02

3.651 ± 0.009

6.071 ± 0.018

2.602 ± 0.007

4.482 ± 0.01

5.669 ± 0.015

9.699 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.006

3.888 ± 0.01

5.646 ± 0.018

4.788 ± 0.014

5.677 ± 0.014

9.185 ± 0.02

5.528 ± 0.013

6.249 ± 0.012

1.122 ± 0.005

2.514 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski