Mycobacterium phage 32HC

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Trigintaduovirus; Mycobacterium virus 32HC

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8EAE3|W8EAE3_9CAUD Uncharacterized protein OS=Mycobacterium phage 32HC OX=1445729 GN=32HC_35 PE=4 SV=1
MM1 pKa = 8.18DD2 pKa = 4.6IANLIAEE9 pKa = 5.43ALPQFCPTTNHH20 pKa = 5.49YY21 pKa = 10.14RR22 pKa = 11.84CSDD25 pKa = 3.26GKK27 pKa = 10.45YY28 pKa = 10.59LLVTVPTIDD37 pKa = 3.36PVGTLAEE44 pKa = 4.09TLGIVVAVAKK54 pKa = 10.21SHH56 pKa = 6.84LPATVDD62 pKa = 3.19VFLSDD67 pKa = 3.51EE68 pKa = 4.2YY69 pKa = 11.65AAVLDD74 pKa = 4.82ADD76 pKa = 4.16GDD78 pKa = 4.23LANGLTPIATFPAGTSHH95 pKa = 7.45AEE97 pKa = 3.58ALALMGYY104 pKa = 8.53SVEE107 pKa = 4.24RR108 pKa = 3.9

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8E8V1|W8E8V1_9CAUD Uncharacterized protein OS=Mycobacterium phage 32HC OX=1445729 GN=32HC_66 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84EE3 pKa = 3.69AKK5 pKa = 9.39TRR7 pKa = 11.84IGATLALVLIGLGAAVAAAFTRR29 pKa = 11.84PGGWLVLLAYY39 pKa = 10.25VVGAACAWLLVTALFAYY56 pKa = 9.75AQLRR60 pKa = 11.84QAVRR64 pKa = 11.84LRR66 pKa = 11.84EE67 pKa = 3.72ALIRR71 pKa = 11.84KK72 pKa = 8.14RR73 pKa = 11.84QTAEE77 pKa = 3.63LARR80 pKa = 11.84FLQQRR85 pKa = 11.84NAAGATPAAPPTNEE99 pKa = 4.05SDD101 pKa = 3.62EE102 pKa = 4.39EE103 pKa = 4.21

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

16394

35

1358

190.6

20.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.315 ± 0.414

1.141 ± 0.152

7.161 ± 0.255

5.465 ± 0.252

2.867 ± 0.165

9.259 ± 0.563

1.812 ± 0.161

4.947 ± 0.199

3.074 ± 0.191

7.692 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.94 ± 0.149

2.934 ± 0.193

6.728 ± 0.272

3.471 ± 0.234

7.454 ± 0.376

5.417 ± 0.207

5.734 ± 0.308

6.484 ± 0.237

1.952 ± 0.149

2.153 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski