Amino acid dipepetide frequency for Amphiprion percula (Orange clownfish) (Lutjanus percula)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.391AlaAla: 6.391 ± 0.028	1.26AlaCys: 1.26 ± 0.012	3.181AlaAsp: 3.181 ± 0.016	4.606AlaGlu: 4.606 ± 0.024	2.408AlaPhe: 2.408 ± 0.013	4.304AlaGly: 4.304 ± 0.022	1.461AlaHis: 1.461 ± 0.009	2.66AlaIle: 2.66 ± 0.014	3.35AlaLys: 3.35 ± 0.016	6.401AlaLeu: 6.401 ± 0.025	1.59AlaMet: 1.59 ± 0.013	2.162AlaAsn: 2.162 ± 0.011	3.426AlaPro: 3.426 ± 0.023	2.885AlaGln: 2.885 ± 0.017	3.012AlaArg: 3.012 ± 0.015	5.445AlaSer: 5.445 ± 0.023	3.503AlaThr: 3.503 ± 0.018	4.938AlaVal: 4.938 ± 0.021	0.63AlaTrp: 0.63 ± 0.006	1.491AlaTyr: 1.491 ± 0.011	0.0AlaXaa: 0.0 ± 0.0
Cys	1.154CysAla: 1.154 ± 0.009	0.716CysCys: 0.716 ± 0.009	1.16CysAsp: 1.16 ± 0.014	1.252CysGlu: 1.252 ± 0.014	0.952CysPhe: 0.952 ± 0.009	1.61CysGly: 1.61 ± 0.017	0.672CysHis: 0.672 ± 0.007	1.011CysIle: 1.011 ± 0.008	1.152CysLys: 1.152 ± 0.011	2.265CysLeu: 2.265 ± 0.019	0.497CysMet: 0.497 ± 0.005	0.854CysAsn: 0.854 ± 0.008	1.312CysPro: 1.312 ± 0.015	1.035CysGln: 1.035 ± 0.01	1.324CysArg: 1.324 ± 0.011	2.239CysSer: 2.239 ± 0.019	1.184CysThr: 1.184 ± 0.01	1.586CysVal: 1.586 ± 0.015	0.327CysTrp: 0.327 ± 0.005	0.652CysTyr: 0.652 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	2.955AspAla: 2.955 ± 0.016	1.17AspCys: 1.17 ± 0.01	3.18AspAsp: 3.18 ± 0.023	3.744AspGlu: 3.744 ± 0.02	2.157AspPhe: 2.157 ± 0.013	3.677AspGly: 3.677 ± 0.018	1.197AspHis: 1.197 ± 0.008	2.693AspIle: 2.693 ± 0.016	2.745AspLys: 2.745 ± 0.016	4.92AspLeu: 4.92 ± 0.022	1.311AspMet: 1.311 ± 0.01	1.963AspAsn: 1.963 ± 0.011	2.833AspPro: 2.833 ± 0.016	2.003AspGln: 2.003 ± 0.011	2.838AspArg: 2.838 ± 0.015	4.523AspSer: 4.523 ± 0.02	2.646AspThr: 2.646 ± 0.015	3.34AspVal: 3.34 ± 0.017	0.694AspTrp: 0.694 ± 0.006	1.564AspTyr: 1.564 ± 0.011	0.0AspXaa: 0.0 ± 0.0
Glu	4.709GluAla: 4.709 ± 0.025	1.205GluCys: 1.205 ± 0.012	4.441GluAsp: 4.441 ± 0.024	7.697GluGlu: 7.697 ± 0.047	1.953GluPhe: 1.953 ± 0.011	4.026GluGly: 4.026 ± 0.019	1.45GluHis: 1.45 ± 0.011	2.802GluIle: 2.802 ± 0.015	4.673GluLys: 4.673 ± 0.03	6.076GluLeu: 6.076 ± 0.028	1.735GluMet: 1.735 ± 0.011	2.744GluAsn: 2.744 ± 0.014	2.79GluPro: 2.79 ± 0.019	3.062GluGln: 3.062 ± 0.018	4.204GluArg: 4.204 ± 0.028	4.25GluSer: 4.25 ± 0.017	3.404GluThr: 3.404 ± 0.02	4.288GluVal: 4.288 ± 0.018	0.679GluTrp: 0.679 ± 0.006	1.574GluTyr: 1.574 ± 0.011	0.0GluXaa: 0.0 ± 0.0
Phe	1.947PheAla: 1.947 ± 0.013	1.03PheCys: 1.03 ± 0.008	1.808PheAsp: 1.808 ± 0.012	1.849PheGlu: 1.849 ± 0.013	1.689PhePhe: 1.689 ± 0.015	2.215PheGly: 2.215 ± 0.015	1.059PheHis: 1.059 ± 0.007	1.987PheIle: 1.987 ± 0.012	1.819PheLys: 1.819 ± 0.011	3.972PheLeu: 3.972 ± 0.02	0.86PheMet: 0.86 ± 0.007	1.513PheAsn: 1.513 ± 0.01	1.835PhePro: 1.835 ± 0.011	1.637PheGln: 1.637 ± 0.011	1.901PheArg: 1.901 ± 0.013	3.529PheSer: 3.529 ± 0.016	2.356PheThr: 2.356 ± 0.014	2.277PheVal: 2.277 ± 0.014	0.491PheTrp: 0.491 ± 0.005	1.247PheTyr: 1.247 ± 0.01	0.0PheXaa: 0.0 ± 0.0
Gly	3.891GlyAla: 3.891 ± 0.02	1.29GlyCys: 1.29 ± 0.013	3.222GlyAsp: 3.222 ± 0.016	4.056GlyGlu: 4.056 ± 0.022	2.447GlyPhe: 2.447 ± 0.014	5.43GlyGly: 5.43 ± 0.038	1.625GlyHis: 1.625 ± 0.013	2.619GlyIle: 2.619 ± 0.015	3.59GlyLys: 3.59 ± 0.019	5.459GlyLeu: 5.459 ± 0.021	1.473GlyMet: 1.473 ± 0.013	2.455GlyAsn: 2.455 ± 0.014	3.171GlyPro: 3.171 ± 0.033	2.714GlyGln: 2.714 ± 0.016	3.643GlyArg: 3.643 ± 0.02	5.681GlySer: 5.681 ± 0.024	3.315GlyThr: 3.315 ± 0.016	3.989GlyVal: 3.989 ± 0.018	0.782GlyTrp: 0.782 ± 0.009	1.817GlyTyr: 1.817 ± 0.013	0.0GlyXaa: 0.0 ± 0.0
His	1.309HisAla: 1.309 ± 0.009	0.778HisCys: 0.778 ± 0.008	0.988HisAsp: 0.988 ± 0.008	1.185HisGlu: 1.185 ± 0.009	1.088HisPhe: 1.088 ± 0.008	1.558HisGly: 1.558 ± 0.015	1.096HisHis: 1.096 ± 0.014	1.338HisIle: 1.338 ± 0.01	1.308HisLys: 1.308 ± 0.009	2.762HisLeu: 2.762 ± 0.015	0.726HisMet: 0.726 ± 0.009	1.042HisAsn: 1.042 ± 0.009	1.632HisPro: 1.632 ± 0.017	1.332HisGln: 1.332 ± 0.014	1.673HisArg: 1.673 ± 0.01	2.525HisSer: 2.525 ± 0.017	1.702HisThr: 1.702 ± 0.023	1.444HisVal: 1.444 ± 0.01	0.348HisTrp: 0.348 ± 0.004	0.872HisTyr: 0.872 ± 0.007	0.0HisXaa: 0.0 ± 0.0
Ile	2.472IleAla: 2.472 ± 0.015	1.129IleCys: 1.129 ± 0.009	2.107IleAsp: 2.107 ± 0.011	2.356IleGlu: 2.356 ± 0.013	1.873IlePhe: 1.873 ± 0.014	2.254IleGly: 2.254 ± 0.013	1.351IleHis: 1.351 ± 0.016	2.444IleIle: 2.444 ± 0.015	2.53IleLys: 2.53 ± 0.014	4.334IleLeu: 4.334 ± 0.02	1.09IleMet: 1.09 ± 0.007	1.966IleAsn: 1.966 ± 0.012	2.452IlePro: 2.452 ± 0.012	2.205IleGln: 2.205 ± 0.012	2.453IleArg: 2.453 ± 0.012	3.805IleSer: 3.805 ± 0.016	2.827IleThr: 2.827 ± 0.015	2.595IleVal: 2.595 ± 0.015	0.485IleTrp: 0.485 ± 0.006	1.44IleTyr: 1.44 ± 0.009	0.0IleXaa: 0.0 ± 0.0
Lys	3.714LysAla: 3.714 ± 0.017	1.034LysCys: 1.034 ± 0.009	3.222LysAsp: 3.222 ± 0.018	4.637LysGlu: 4.637 ± 0.028	1.617LysPhe: 1.617 ± 0.011	3.053LysGly: 3.053 ± 0.018	1.446LysHis: 1.446 ± 0.011	2.468LysIle: 2.468 ± 0.015	4.466LysLys: 4.466 ± 0.03	4.964LysLeu: 4.964 ± 0.02	1.495LysMet: 1.495 ± 0.01	2.261LysAsn: 2.261 ± 0.012	2.986LysPro: 2.986 ± 0.021	2.568LysGln: 2.568 ± 0.017	3.386LysArg: 3.386 ± 0.018	3.827LysSer: 3.827 ± 0.019	3.276LysThr: 3.276 ± 0.017	3.546LysVal: 3.546 ± 0.018	0.565LysTrp: 0.565 ± 0.006	1.512LysTyr: 1.512 ± 0.012	0.0LysXaa: 0.0 ± 0.0
Leu	5.96LeuAla: 5.96 ± 0.024	2.268LeuCys: 2.268 ± 0.015	4.875LeuAsp: 4.875 ± 0.021	6.309LeuGlu: 6.309 ± 0.03	3.48LeuPhe: 3.48 ± 0.018	5.086LeuGly: 5.086 ± 0.02	2.785LeuHis: 2.785 ± 0.015	3.887LeuIle: 3.887 ± 0.021	5.582LeuLys: 5.582 ± 0.026	10.129LeuLeu: 10.129 ± 0.045	2.14LeuMet: 2.14 ± 0.012	3.648LeuAsn: 3.648 ± 0.015	5.287LeuPro: 5.287 ± 0.022	5.519LeuGln: 5.519 ± 0.029	5.558LeuArg: 5.558 ± 0.024	8.362LeuSer: 8.362 ± 0.035	5.318LeuThr: 5.318 ± 0.02	5.547LeuVal: 5.547 ± 0.021	1.104LeuTrp: 1.104 ± 0.011	2.653LeuTyr: 2.653 ± 0.014	0.0LeuXaa: 0.0 ± 0.0
Met	1.906MetAla: 1.906 ± 0.011	0.5MetCys: 0.5 ± 0.006	1.447MetAsp: 1.447 ± 0.01	1.99MetGlu: 1.99 ± 0.012	0.91MetPhe: 0.91 ± 0.007	1.429MetGly: 1.429 ± 0.012	0.492MetHis: 0.492 ± 0.005	0.899MetIle: 0.899 ± 0.007	1.52MetLys: 1.52 ± 0.009	2.127MetLeu: 2.127 ± 0.012	0.741MetMet: 0.741 ± 0.009	0.938MetAsn: 0.938 ± 0.008	1.082MetPro: 1.082 ± 0.01	1.01MetGln: 1.01 ± 0.01	1.231MetArg: 1.231 ± 0.01	1.919MetSer: 1.919 ± 0.012	1.296MetThr: 1.296 ± 0.009	1.576MetVal: 1.576 ± 0.01	0.275MetTrp: 0.275 ± 0.004	0.645MetTyr: 0.645 ± 0.007	0.0MetXaa: 0.0 ± 0.0
Asn	2.159AsnAla: 2.159 ± 0.014	0.878AsnCys: 0.878 ± 0.008	1.696AsnAsp: 1.696 ± 0.011	2.039AsnGlu: 2.039 ± 0.014	1.431AsnPhe: 1.431 ± 0.011	2.711AsnGly: 2.711 ± 0.018	1.025AsnHis: 1.025 ± 0.008	2.181AsnIle: 2.181 ± 0.013	2.223AsnLys: 2.223 ± 0.014	3.645AsnLeu: 3.645 ± 0.017	1.079AsnMet: 1.079 ± 0.009	1.825AsnAsn: 1.825 ± 0.014	2.252AsnPro: 2.252 ± 0.014	1.809AsnGln: 1.809 ± 0.012	1.996AsnArg: 1.996 ± 0.012	3.244AsnSer: 3.244 ± 0.016	2.292AsnThr: 2.292 ± 0.013	2.334AsnVal: 2.334 ± 0.015	0.447AsnTrp: 0.447 ± 0.006	1.137AsnTyr: 1.137 ± 0.009	0.0AsnXaa: 0.0 ± 0.0
Pro	4.169ProAla: 4.169 ± 0.024	1.05ProCys: 1.05 ± 0.012	2.903ProAsp: 2.903 ± 0.016	3.749ProGlu: 3.749 ± 0.018	1.819ProPhe: 1.819 ± 0.012	3.982ProGly: 3.982 ± 0.033	1.528ProHis: 1.528 ± 0.013	1.879ProIle: 1.879 ± 0.011	2.498ProLys: 2.498 ± 0.017	4.812ProLeu: 4.812 ± 0.02	1.019ProMet: 1.019 ± 0.01	1.918ProAsn: 1.918 ± 0.012	5.514ProPro: 5.514 ± 0.05	2.682ProGln: 2.682 ± 0.021	2.687ProArg: 2.687 ± 0.017	5.544ProSer: 5.544 ± 0.029	3.116ProThr: 3.116 ± 0.018	3.804ProVal: 3.804 ± 0.023	0.546ProTrp: 0.546 ± 0.007	1.458ProTyr: 1.458 ± 0.012	0.0ProXaa: 0.0 ± 0.0
Gln	3.101GlnAla: 3.101 ± 0.017	0.996GlnCys: 0.996 ± 0.012	2.345GlnAsp: 2.345 ± 0.013	3.472GlnGlu: 3.472 ± 0.022	1.363GlnPhe: 1.363 ± 0.009	2.677GlnGly: 2.677 ± 0.017	1.408GlnHis: 1.408 ± 0.015	1.935GlnIle: 1.935 ± 0.011	2.622GlnLys: 2.622 ± 0.016	4.537GlnLeu: 4.537 ± 0.023	1.165GlnMet: 1.165 ± 0.009	1.812GlnAsn: 1.812 ± 0.014	2.606GlnPro: 2.606 ± 0.019	3.43GlnGln: 3.43 ± 0.037	3.123GlnArg: 3.123 ± 0.016	3.495GlnSer: 3.495 ± 0.016	2.648GlnThr: 2.648 ± 0.015	2.823GlnVal: 2.823 ± 0.015	0.55GlnTrp: 0.55 ± 0.006	1.247GlnTyr: 1.247 ± 0.009	0.0GlnXaa: 0.0 ± 0.0
Arg	3.344ArgAla: 3.344 ± 0.016	1.323ArgCys: 1.323 ± 0.013	2.915ArgAsp: 2.915 ± 0.015	3.859ArgGlu: 3.859 ± 0.023	2.02ArgPhe: 2.02 ± 0.011	3.435ArgGly: 3.435 ± 0.02	1.6ArgHis: 1.6 ± 0.012	2.4ArgIle: 2.4 ± 0.013	3.505ArgLys: 3.505 ± 0.017	5.324ArgLeu: 5.324 ± 0.021	1.306ArgMet: 1.306 ± 0.01	2.128ArgAsn: 2.128 ± 0.011	2.964ArgPro: 2.964 ± 0.017	2.677ArgGln: 2.677 ± 0.013	4.407ArgArg: 4.407 ± 0.024	4.542ArgSer: 4.542 ± 0.025	2.985ArgThr: 2.985 ± 0.016	3.251ArgVal: 3.251 ± 0.015	0.703ArgTrp: 0.703 ± 0.006	1.583ArgTyr: 1.583 ± 0.011	0.0ArgXaa: 0.0 ± 0.0
Ser	5.598SerAla: 5.598 ± 0.021	2.134SerCys: 2.134 ± 0.019	4.404SerAsp: 4.404 ± 0.023	4.823SerGlu: 4.823 ± 0.023	3.207SerPhe: 3.207 ± 0.014	5.625SerGly: 5.625 ± 0.021	2.281SerHis: 2.281 ± 0.014	3.416SerIle: 3.416 ± 0.018	3.971SerLys: 3.971 ± 0.02	8.296SerLeu: 8.296 ± 0.03	1.856SerMet: 1.856 ± 0.011	3.013SerAsn: 3.013 ± 0.016	5.916SerPro: 5.916 ± 0.031	3.874SerGln: 3.874 ± 0.021	4.579SerArg: 4.579 ± 0.024	10.541SerSer: 10.541 ± 0.049	4.893SerThr: 4.893 ± 0.023	5.558SerVal: 5.558 ± 0.022	1.016SerTrp: 1.016 ± 0.009	2.205SerTyr: 2.205 ± 0.013	0.0SerXaa: 0.0 ± 0.0
Thr	4.08ThrAla: 4.08 ± 0.02	1.401ThrCys: 1.401 ± 0.014	2.976ThrAsp: 2.976 ± 0.016	3.759ThrGlu: 3.759 ± 0.02	2.164ThrPhe: 2.164 ± 0.013	3.753ThrGly: 3.753 ± 0.019	1.534ThrHis: 1.534 ± 0.024	2.438ThrIle: 2.438 ± 0.014	2.69ThrLys: 2.69 ± 0.014	5.337ThrLeu: 5.337 ± 0.021	1.227ThrMet: 1.227 ± 0.008	1.984ThrAsn: 1.984 ± 0.013	3.58ThrPro: 3.58 ± 0.023	2.36ThrGln: 2.36 ± 0.015	2.514ThrArg: 2.514 ± 0.015	4.942ThrSer: 4.942 ± 0.024	3.481ThrThr: 3.481 ± 0.035	4.255ThrVal: 4.255 ± 0.021	0.672ThrTrp: 0.672 ± 0.007	1.46ThrTyr: 1.46 ± 0.011	0.0ThrXaa: 0.0 ± 0.0
Val	4.152ValAla: 4.152 ± 0.017	1.807ValCys: 1.807 ± 0.017	3.238ValAsp: 3.238 ± 0.015	4.05ValGlu: 4.05 ± 0.023	2.748ValPhe: 2.748 ± 0.017	3.532ValGly: 3.532 ± 0.017	1.617ValHis: 1.617 ± 0.011	3.017ValIle: 3.017 ± 0.017	3.608ValLys: 3.608 ± 0.016	6.283ValLeu: 6.283 ± 0.027	1.565ValMet: 1.565 ± 0.01	2.447ValAsn: 2.447 ± 0.016	3.268ValPro: 3.268 ± 0.019	2.802ValGln: 2.802 ± 0.015	3.284ValArg: 3.284 ± 0.016	5.412ValSer: 5.412 ± 0.024	3.994ValThr: 3.994 ± 0.018	4.532ValVal: 4.532 ± 0.026	0.78ValTrp: 0.78 ± 0.007	1.858ValTyr: 1.858 ± 0.011	0.0ValXaa: 0.0 ± 0.0
Trp	0.668TrpAla: 0.668 ± 0.007	0.246TrpCys: 0.246 ± 0.004	0.663TrpAsp: 0.663 ± 0.007	0.739TrpGlu: 0.739 ± 0.008	0.475TrpPhe: 0.475 ± 0.006	0.644TrpGly: 0.644 ± 0.01	0.275TrpHis: 0.275 ± 0.004	0.586TrpIle: 0.586 ± 0.007	0.721TrpLys: 0.721 ± 0.008	1.167TrpLeu: 1.167 ± 0.01	0.361TrpMet: 0.361 ± 0.005	0.508TrpAsn: 0.508 ± 0.005	0.435TrpPro: 0.435 ± 0.006	0.489TrpGln: 0.489 ± 0.006	0.765TrpArg: 0.765 ± 0.007	0.977TrpSer: 0.977 ± 0.01	0.73TrpThr: 0.73 ± 0.008	0.705TrpVal: 0.705 ± 0.006	0.196TrpTrp: 0.196 ± 0.004	0.342TrpTyr: 0.342 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.423TyrAla: 1.423 ± 0.009	0.738TyrCys: 0.738 ± 0.007	1.386TyrAsp: 1.386 ± 0.01	1.59TyrGlu: 1.59 ± 0.01	1.222TyrPhe: 1.222 ± 0.01	1.67TyrGly: 1.67 ± 0.011	0.805TyrHis: 0.805 ± 0.007	1.509TyrIle: 1.509 ± 0.011	1.469TyrLys: 1.469 ± 0.012	2.686TyrLeu: 2.686 ± 0.017	0.695TyrMet: 0.695 ± 0.006	1.219TyrAsn: 1.219 ± 0.009	1.337TyrPro: 1.337 ± 0.011	1.263TyrGln: 1.263 ± 0.009	1.693TyrArg: 1.693 ± 0.011	2.405TyrSer: 2.405 ± 0.011	1.641TyrThr: 1.641 ± 0.011	1.607TyrVal: 1.607 ± 0.011	0.402TyrTrp: 0.402 ± 0.005	1.021TyrTyr: 1.021 ± 0.01	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.001

Statistics based on 33681 proteins (18269780 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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