Walleye dermal sarcoma virus (WDSV)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Epsilonretrovirus

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q88940|ORFB_WDSV ORF-B protein OS=Walleye dermal sarcoma virus OX=39720 PE=1 SV=1
MM1 pKa = 7.42FSDD4 pKa = 4.06SDD6 pKa = 3.96SSDD9 pKa = 3.57EE10 pKa = 3.95EE11 pKa = 3.94LSRR14 pKa = 11.84IITDD18 pKa = 3.06IDD20 pKa = 3.84EE21 pKa = 4.57SPQDD25 pKa = 3.56IQQSLHH31 pKa = 5.68LRR33 pKa = 11.84AGVSPEE39 pKa = 3.48ARR41 pKa = 11.84VPPGGLTQEE50 pKa = 4.37EE51 pKa = 4.51WTIDD55 pKa = 3.64YY56 pKa = 9.99FSTYY60 pKa = 8.21HH61 pKa = 6.24TPLLNPGIPHH71 pKa = 7.5EE72 pKa = 4.07LTQRR76 pKa = 11.84CTDD79 pKa = 3.45YY80 pKa = 10.12TASILRR86 pKa = 11.84RR87 pKa = 11.84ASAKK91 pKa = 9.57MIQWDD96 pKa = 4.05YY97 pKa = 11.76VFYY100 pKa = 10.89LLPRR104 pKa = 11.84VWIMFPFIARR114 pKa = 11.84EE115 pKa = 4.08GLSHH119 pKa = 6.85LTHH122 pKa = 7.41LLTLTTSVLSATSLVFGWDD141 pKa = 3.09LTVIEE146 pKa = 5.52LCNEE150 pKa = 3.77MNIQGVYY157 pKa = 9.81LPEE160 pKa = 4.47VIEE163 pKa = 4.14WLAQFSFLFTHH174 pKa = 5.57VTLIVVSDD182 pKa = 3.26GMMDD186 pKa = 4.72LLLMFPMDD194 pKa = 4.42IEE196 pKa = 4.23EE197 pKa = 4.3QPLAINIALHH207 pKa = 6.64ALQTSYY213 pKa = 11.27TIMTPILFASPLLRR227 pKa = 11.84IISCVLYY234 pKa = 10.83ACGHH238 pKa = 6.1CPSARR243 pKa = 11.84MLYY246 pKa = 10.26AYY248 pKa = 9.41TIMNRR253 pKa = 11.84YY254 pKa = 6.85TGEE257 pKa = 4.47SIAEE261 pKa = 3.82MHH263 pKa = 6.17TGFRR267 pKa = 11.84CFRR270 pKa = 11.84DD271 pKa = 3.35QMIAYY276 pKa = 10.07DD277 pKa = 3.7MEE279 pKa = 4.33FTNFLRR285 pKa = 11.84DD286 pKa = 3.54LTEE289 pKa = 3.94EE290 pKa = 4.04EE291 pKa = 4.74TPVLEE296 pKa = 4.11ITEE299 pKa = 4.59PEE301 pKa = 4.14PSPTEE306 pKa = 3.71

Molecular weight: 34.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q88937|GAG_WDSV Gag polyprotein OS=Walleye dermal sarcoma virus OX=39720 GN=gag PE=1 SV=1
MM1 pKa = 7.41AWYY4 pKa = 8.86HH5 pKa = 4.09QHH7 pKa = 6.47RR8 pKa = 11.84WHH10 pKa = 7.21LDD12 pKa = 3.19YY13 pKa = 11.11SIPRR17 pKa = 11.84QNLQAFLTTITFIDD31 pKa = 4.01PQFKK35 pKa = 10.05IQEE38 pKa = 4.14NGLTEE43 pKa = 4.6GEE45 pKa = 4.44YY46 pKa = 9.08KK47 pKa = 9.1TQIVKK52 pKa = 10.34QIIPQLCRR60 pKa = 11.84IPNQNSPPPIWVQGPRR76 pKa = 11.84IKK78 pKa = 10.85GDD80 pKa = 3.67PTWLKK85 pKa = 10.71INAKK89 pKa = 10.0FITEE93 pKa = 5.42LIPKK97 pKa = 9.79QKK99 pKa = 8.72GTKK102 pKa = 9.81NISTKK107 pKa = 10.15TYY109 pKa = 9.75LSRR112 pKa = 11.84LFVIWLQNN120 pKa = 3.18

Molecular weight: 14.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4217

120

1752

702.8

79.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.758 ± 0.709

1.66 ± 0.142

4.34 ± 0.461

5.193 ± 0.64

2.988 ± 0.25

4.743 ± 0.186

3.106 ± 0.316

7.209 ± 0.595

5.834 ± 0.921

9.153 ± 0.711

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.537 ± 0.292

4.387 ± 0.571

6.995 ± 0.38

6.782 ± 0.748

4.387 ± 0.219

5.762 ± 0.41

8.229 ± 0.446

5.478 ± 0.311

1.613 ± 0.137

2.822 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski