Hubei rhabdo-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMW7|A0A1L3KMW7_9VIRU Uncharacterized protein OS=Hubei rhabdo-like virus 1 OX=1923185 PE=4 SV=1
MM1 pKa = 7.16YY2 pKa = 9.54PSEE5 pKa = 4.16TRR7 pKa = 11.84ILIATVIIIAIFGIIIILFCWCLAKK32 pKa = 10.12RR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 9.24PGHH38 pKa = 6.34HH39 pKa = 6.35LTRR42 pKa = 11.84PHH44 pKa = 6.48RR45 pKa = 11.84CKK47 pKa = 10.22FHH49 pKa = 5.88YY50 pKa = 10.31SRR52 pKa = 11.84LRR54 pKa = 11.84DD55 pKa = 3.64LEE57 pKa = 4.11NQRR60 pKa = 11.84VTSGSSSAATSYY72 pKa = 11.24VSTPRR77 pKa = 11.84YY78 pKa = 8.55PADD81 pKa = 3.6SDD83 pKa = 3.78SSRR86 pKa = 11.84SVLTRR91 pKa = 11.84RR92 pKa = 11.84MSSLSDD98 pKa = 3.26KK99 pKa = 10.6LRR101 pKa = 11.84QIQEE105 pKa = 3.9EE106 pKa = 4.21DD107 pKa = 3.63PEE109 pKa = 4.37LKK111 pKa = 10.37RR112 pKa = 11.84RR113 pKa = 11.84FEE115 pKa = 4.38EE116 pKa = 3.89IKK118 pKa = 10.54IRR120 pKa = 11.84GADD123 pKa = 3.69SLSTPSDD130 pKa = 3.27ADD132 pKa = 3.81DD133 pKa = 3.7LVQRR137 pKa = 11.84SLRR140 pKa = 11.84GFSSEE145 pKa = 4.71SEE147 pKa = 3.63ILYY150 pKa = 10.37SLNYY154 pKa = 9.8LPSDD158 pKa = 3.9LEE160 pKa = 4.16VEE162 pKa = 4.53AGVEE166 pKa = 4.15TQRR169 pKa = 11.84TGEE172 pKa = 4.0GDD174 pKa = 3.26NTGGGTAGSSQSTSTQGDD192 pKa = 4.21CGPVLDD198 pKa = 5.37PYY200 pKa = 9.5WAAVLNSNFNQGHH213 pKa = 6.29PRR215 pKa = 11.84DD216 pKa = 3.6HH217 pKa = 6.4LQRR220 pKa = 11.84LGALAKK226 pKa = 10.24DD227 pKa = 4.33HH228 pKa = 7.67PDD230 pKa = 3.28PDD232 pKa = 3.75IPNIWSEE239 pKa = 3.93ITPIFQDD246 pKa = 3.78LLKK249 pKa = 10.84ANQIMLLRR257 pKa = 11.84LLLGEE262 pKa = 4.65LRR264 pKa = 11.84NPVQDD269 pKa = 2.05IRR271 pKa = 11.84MFIRR275 pKa = 11.84GVQFGHH281 pKa = 5.96QMRR284 pKa = 11.84EE285 pKa = 3.82YY286 pKa = 11.09SLLQAHH292 pKa = 5.94TLHH295 pKa = 6.74LEE297 pKa = 4.06SLIDD301 pKa = 3.54TMNVNNAKK309 pKa = 10.09VVGRR313 pKa = 11.84LEE315 pKa = 4.57ASMNQNAALASQVTEE330 pKa = 3.6ASKK333 pKa = 11.19NVTHH337 pKa = 6.86FVAAVEE343 pKa = 4.17KK344 pKa = 10.62AQLLAEE350 pKa = 4.23KK351 pKa = 9.68MEE353 pKa = 4.68LSLVPPEE360 pKa = 4.08VVPASKK366 pKa = 10.11HH367 pKa = 4.83AAPSIISRR375 pKa = 11.84VTEE378 pKa = 3.9TQKK381 pKa = 11.42AYY383 pKa = 9.37VTLDD387 pKa = 3.01AKK389 pKa = 11.03LGDD392 pKa = 3.7GVYY395 pKa = 9.94EE396 pKa = 4.25SKK398 pKa = 10.5IGLVTIVAEE407 pKa = 4.51KK408 pKa = 9.6VRR410 pKa = 11.84SVAIHH415 pKa = 6.87AGLNDD420 pKa = 3.24PWMPLVGMDD429 pKa = 3.5ARR431 pKa = 11.84VFQIMACVNLDD442 pKa = 3.57TLEE445 pKa = 4.94SKK447 pKa = 10.35LQPGVLASIFTGSSEE462 pKa = 4.11EE463 pKa = 4.16KK464 pKa = 9.93NSKK467 pKa = 10.09FGALIGSCGSAAMQWWFATRR487 pKa = 11.84VDD489 pKa = 4.0EE490 pKa = 5.56SMHH493 pKa = 7.15DD494 pKa = 3.91DD495 pKa = 3.51LSGEE499 pKa = 4.21SSSAA503 pKa = 3.16

Molecular weight: 55.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMV5|A0A1L3KMV5_9VIRU Uncharacterized protein OS=Hubei rhabdo-like virus 1 OX=1923185 PE=4 SV=1
MM1 pKa = 7.4TNLIRR6 pKa = 11.84RR7 pKa = 11.84FTRR10 pKa = 11.84SSKK13 pKa = 10.7ANKK16 pKa = 9.4VDD18 pKa = 3.58PQNNSPYY25 pKa = 9.32PPKK28 pKa = 10.53SFKK31 pKa = 9.83FTLEE35 pKa = 4.06HH36 pKa = 5.82IRR38 pKa = 11.84AAVALEE44 pKa = 3.75GVINFDD50 pKa = 3.0IEE52 pKa = 4.51GPIEE56 pKa = 4.13YY57 pKa = 9.25TVIPYY62 pKa = 9.91ISKK65 pKa = 10.66RR66 pKa = 11.84MVAYY70 pKa = 9.81ILRR73 pKa = 11.84IAIDD77 pKa = 3.04QHH79 pKa = 7.97IITEE83 pKa = 4.12EE84 pKa = 3.55AATYY88 pKa = 10.5FDD90 pKa = 3.66APLRR94 pKa = 11.84KK95 pKa = 9.61AIIQSAPYY103 pKa = 8.3QTSKK107 pKa = 10.63EE108 pKa = 3.94KK109 pKa = 10.88KK110 pKa = 8.53LIHH113 pKa = 7.1RR114 pKa = 11.84ITGVAPVKK122 pKa = 10.58LLTPVSCKK130 pKa = 9.9VVADD134 pKa = 4.18KK135 pKa = 11.3EE136 pKa = 4.48SLTWDD141 pKa = 3.56KK142 pKa = 8.39TTEE145 pKa = 4.18MIGKK149 pKa = 9.19ALYY152 pKa = 9.42PVVIRR157 pKa = 11.84CFGEE161 pKa = 4.36LNTSSFLLRR170 pKa = 11.84DD171 pKa = 3.37RR172 pKa = 11.84GLLRR176 pKa = 11.84GRR178 pKa = 11.84MFLSTPIPSPQQVAKK193 pKa = 9.32TKK195 pKa = 10.06SAIRR199 pKa = 11.84KK200 pKa = 7.17EE201 pKa = 4.03RR202 pKa = 11.84ARR204 pKa = 11.84ALAIEE209 pKa = 4.48DD210 pKa = 4.17TPGPSAPTLDD220 pKa = 3.44

Molecular weight: 24.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3790

220

2112

758.0

84.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.623 ± 0.863

1.319 ± 0.161

4.538 ± 0.227

5.858 ± 0.139

4.195 ± 0.345

6.332 ± 0.514

2.559 ± 0.283

6.174 ± 0.512

4.855 ± 0.413

10.712 ± 0.559

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.165

3.43 ± 0.236

5.778 ± 0.346

3.562 ± 0.227

5.884 ± 0.275

9.024 ± 0.593

6.332 ± 0.338

6.332 ± 0.231

1.504 ± 0.155

2.77 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski