Grapevine rupestris stem pitting-associated virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Foveavirus; Grapevine rupestris stem pitting-associated virus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O91904|O91904_9VIRU 28 kDa coat protein OS=Grapevine rupestris stem pitting-associated virus 1 OX=81832 PE=4 SV=1
MM1 pKa = 7.16YY2 pKa = 9.92CLFGILVLVGIVIAIQILAHH22 pKa = 6.17VDD24 pKa = 3.41SSSGNHH30 pKa = 4.99QGCFIRR36 pKa = 11.84ATGEE40 pKa = 3.92SILIEE45 pKa = 3.94NCGPSEE51 pKa = 4.0ALASTVKK58 pKa = 10.37EE59 pKa = 3.89VLGGLKK65 pKa = 10.56ALGVSRR71 pKa = 11.84AVEE74 pKa = 4.47EE75 pKa = 4.28IDD77 pKa = 3.53YY78 pKa = 10.73HH79 pKa = 6.29CC80 pKa = 5.34

Molecular weight: 8.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q77WZ0|Q77WZ0_9VIRU Movement protein TGB2 OS=Grapevine rupestris stem pitting-associated virus 1 OX=81832 PE=3 SV=1
MM1 pKa = 8.02PFQQPANWAKK11 pKa = 10.07TITPLTVGLGIGLVLHH27 pKa = 6.72FLRR30 pKa = 11.84KK31 pKa = 9.83SNLPYY36 pKa = 10.76SGDD39 pKa = 3.76NIHH42 pKa = 6.14QFPHH46 pKa = 6.08GGRR49 pKa = 11.84YY50 pKa = 8.89RR51 pKa = 11.84DD52 pKa = 3.66GTKK55 pKa = 10.39SITYY59 pKa = 9.12CGPKK63 pKa = 9.85QSFPSSGIFGQSEE76 pKa = 4.31NFVPLMLVIGLIAFIHH92 pKa = 5.94VLSVWNSGLGRR103 pKa = 11.84NCNCHH108 pKa = 6.74PNPCSCRR115 pKa = 11.84QQQ117 pKa = 3.08

Molecular weight: 12.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2838

80

2161

567.6

63.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.166 ± 0.994

2.713 ± 0.41

4.545 ± 0.623

6.73 ± 0.834

6.589 ± 0.754

6.801 ± 1.073

2.713 ± 0.284

6.237 ± 0.906

5.99 ± 0.611

10.113 ± 0.862

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.327

4.651 ± 0.346

4.017 ± 0.765

2.643 ± 0.399

5.25 ± 0.575

9.479 ± 0.384

3.77 ± 0.179

6.061 ± 0.994

0.951 ± 0.154

2.396 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski