Novimethylophilus kurashikiensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Methylophilaceae; Novimethylophilus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3579 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R5FBC0|A0A2R5FBC0_9PROT Delta-aminolevulinic acid dehydratase OS=Novimethylophilus kurashikiensis OX=1825523 GN=hemB PE=3 SV=1
MM1 pKa = 7.83SDD3 pKa = 3.31DD4 pKa = 3.22TTTAFFANLVYY15 pKa = 9.74DD16 pKa = 3.65TGFDD20 pKa = 3.33KK21 pKa = 10.83SIQMGMVVEE30 pKa = 4.66SPDD33 pKa = 3.47GNLWTVLKK41 pKa = 10.06TSDD44 pKa = 3.48VNLQGFQAAAYY55 pKa = 9.41QNIEE59 pKa = 3.83TGQIIVATRR68 pKa = 11.84GTEE71 pKa = 3.94PTSKK75 pKa = 9.83MDD77 pKa = 4.9LYY79 pKa = 11.58ADD81 pKa = 4.03LQMGMDD87 pKa = 4.23GVLAGLPNQFVLASNFLSSVEE108 pKa = 4.56AIAGANPISLTGHH121 pKa = 5.86SLGGSLAEE129 pKa = 3.81WLGAEE134 pKa = 3.99SHH136 pKa = 6.43YY137 pKa = 9.91PTVTYY142 pKa = 10.33NAYY145 pKa = 10.52GIGTILAANLMLPDD159 pKa = 5.43DD160 pKa = 4.71SDD162 pKa = 3.67FSNIVNNVIDD172 pKa = 4.7KK173 pKa = 10.59DD174 pKa = 4.04PVMMSCMGLPQATLTSSII192 pKa = 4.19

Molecular weight: 20.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R5F6C0|A0A2R5F6C0_9PROT Uncharacterized protein OS=Novimethylophilus kurashikiensis OX=1825523 GN=NMK_0666 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 8.87VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.4TKK25 pKa = 10.4GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.07GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3579

0

3579

1118851

10

3516

312.6

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.519 ± 0.049

0.949 ± 0.019

5.404 ± 0.036

6.039 ± 0.042

3.705 ± 0.026

7.567 ± 0.042

2.414 ± 0.024

5.436 ± 0.032

4.455 ± 0.037

10.52 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.019

3.449 ± 0.032

4.64 ± 0.035

4.121 ± 0.027

5.783 ± 0.042

5.807 ± 0.037

5.288 ± 0.056

7.203 ± 0.035

1.326 ± 0.019

2.687 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski