Nosema ceranae (strain BRL01) (Microsporidian parasite)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Apansporoblastina; Nosematidae; Nosema; Nosema ceranae

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2060 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4V8R8|C4V8R8_NOSCE Uncharacterized protein OS=Nosema ceranae (strain BRL01) OX=578460 GN=NCER_100907 PE=4 SV=1
MM1 pKa = 7.14TKK3 pKa = 10.23FYY5 pKa = 10.34PSYY8 pKa = 10.76KK9 pKa = 10.38DD10 pKa = 3.52YY11 pKa = 11.21FEE13 pKa = 4.83KK14 pKa = 11.02LQDD17 pKa = 3.48IQDD20 pKa = 3.56LDD22 pKa = 4.37FYY24 pKa = 11.65DD25 pKa = 3.98EE26 pKa = 4.33VDD28 pKa = 3.28IKK30 pKa = 11.45DD31 pKa = 3.52FTFDD35 pKa = 3.87KK36 pKa = 11.04DD37 pKa = 3.64NSRR40 pKa = 11.84LTYY43 pKa = 9.61PCPCGDD49 pKa = 3.5LFEE52 pKa = 5.61IFIEE56 pKa = 4.38DD57 pKa = 4.13LLNHH61 pKa = 6.7EE62 pKa = 4.99DD63 pKa = 3.55VARR66 pKa = 11.84CPSCSLIIKK75 pKa = 9.88IVYY78 pKa = 9.9EE79 pKa = 4.88DD80 pKa = 3.7IDD82 pKa = 5.03LEE84 pKa = 4.62DD85 pKa = 4.02FLL87 pKa = 6.81

Molecular weight: 10.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4V9R8|C4V9R8_NOSCE Aldo_ket_red domain-containing protein OS=Nosema ceranae (strain BRL01) OX=578460 GN=NCER_101329 PE=4 SV=1
MM1 pKa = 7.52AAADD5 pKa = 4.34YY6 pKa = 10.6IRR8 pKa = 11.84EE9 pKa = 4.15IQKK12 pKa = 10.5SKK14 pKa = 10.67QSDD17 pKa = 2.95NMRR20 pKa = 11.84YY21 pKa = 9.46LLRR24 pKa = 11.84LRR26 pKa = 11.84VCEE29 pKa = 3.63YY30 pKa = 9.55RR31 pKa = 11.84QRR33 pKa = 11.84GEE35 pKa = 3.77VFRR38 pKa = 11.84IPRR41 pKa = 11.84PTFLQRR47 pKa = 11.84ARR49 pKa = 11.84TLGYY53 pKa = 8.04RR54 pKa = 11.84TKK56 pKa = 10.84EE57 pKa = 4.13GILCYY62 pKa = 9.65IARR65 pKa = 11.84VKK67 pKa = 10.47KK68 pKa = 10.21GNRR71 pKa = 11.84KK72 pKa = 9.27RR73 pKa = 11.84EE74 pKa = 4.07YY75 pKa = 10.82NNGNTRR81 pKa = 11.84GKK83 pKa = 10.03CKK85 pKa = 9.95NARR88 pKa = 11.84IHH90 pKa = 6.32QITPAVRR97 pKa = 11.84HH98 pKa = 5.24QATAEE103 pKa = 3.96QIVGKK108 pKa = 9.4RR109 pKa = 11.84HH110 pKa = 5.48PNLRR114 pKa = 11.84VLNSYY119 pKa = 8.13WVNQDD124 pKa = 3.21LVYY127 pKa = 10.7KK128 pKa = 8.38YY129 pKa = 10.53FEE131 pKa = 4.62VIMVDD136 pKa = 3.56PNHH139 pKa = 6.01NAIRR143 pKa = 11.84NDD145 pKa = 3.36PKK147 pKa = 11.28LNWICKK153 pKa = 8.85GVMKK157 pKa = 10.5HH158 pKa = 5.47RR159 pKa = 11.84EE160 pKa = 4.16CRR162 pKa = 11.84GLTSASKK169 pKa = 10.02KK170 pKa = 10.39SRR172 pKa = 11.84GLGHH176 pKa = 7.19GIKK179 pKa = 10.6YY180 pKa = 10.51NNTIGGSVRR189 pKa = 11.84ACWKK193 pKa = 10.53RR194 pKa = 11.84NVTTSLRR201 pKa = 11.84RR202 pKa = 11.84FRR204 pKa = 5.05

Molecular weight: 23.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2060

0

2060

672377

37

3393

326.4

38.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.02 ± 0.043

2.07 ± 0.021

5.773 ± 0.037

6.819 ± 0.052

6.162 ± 0.058

3.522 ± 0.046

1.656 ± 0.019

9.728 ± 0.061

10.397 ± 0.063

10.014 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.995 ± 0.021

7.93 ± 0.063

2.428 ± 0.036

2.688 ± 0.028

3.371 ± 0.034

6.995 ± 0.045

4.809 ± 0.033

5.155 ± 0.042

0.567 ± 0.012

4.9 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski