Grapevine geminivirus A

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Maldovirus

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8GZG7|A0A1D8GZG7_9GEMI TrAP/C2 OS=Grapevine geminivirus A OX=1906317 PE=3 SV=1
MM1 pKa = 7.52WLVFMDD7 pKa = 4.1RR8 pKa = 11.84LYY10 pKa = 11.54SSFQDD15 pKa = 3.51KK16 pKa = 10.15RR17 pKa = 11.84LHH19 pKa = 5.8PAEE22 pKa = 4.5ILPHH26 pKa = 4.6VTQWICEE33 pKa = 4.11KK34 pKa = 10.24GTAQQLIDD42 pKa = 4.72WIHH45 pKa = 5.99GLQSEE50 pKa = 4.44KK51 pKa = 11.27EE52 pKa = 4.24EE53 pKa = 4.12IFRR56 pKa = 11.84TLDD59 pKa = 3.28ACSVGPSEE67 pKa = 4.29EE68 pKa = 4.13MEE70 pKa = 4.4GFGGPRR76 pKa = 11.84GQEE79 pKa = 3.96KK80 pKa = 10.69VDD82 pKa = 4.05LSLTLSSPTDD92 pKa = 3.19EE93 pKa = 4.43RR94 pKa = 11.84PQVKK98 pKa = 9.76SVSISS103 pKa = 3.03

Molecular weight: 11.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8GZG8|A0A1D8GZG8_9GEMI Replication enhancer OS=Grapevine geminivirus A OX=1906317 PE=3 SV=1
MM1 pKa = 7.55PVGHH5 pKa = 6.42VRR7 pKa = 11.84VLDD10 pKa = 3.69SRR12 pKa = 11.84TGEE15 pKa = 4.29SLTSQQTQTGVYY27 pKa = 9.52SWTLKK32 pKa = 10.31NPLYY36 pKa = 9.94VTIIQSWDD44 pKa = 2.74IFLPKK49 pKa = 10.64GKK51 pKa = 10.09GFKK54 pKa = 9.78IQFRR58 pKa = 11.84ANHH61 pKa = 5.57NMRR64 pKa = 11.84KK65 pKa = 9.63ALGLHH70 pKa = 6.1KK71 pKa = 10.42CWITYY76 pKa = 8.56RR77 pKa = 11.84VWTTLKK83 pKa = 9.85TFGLISLQIRR93 pKa = 11.84IRR95 pKa = 11.84DD96 pKa = 3.84RR97 pKa = 11.84ILSYY101 pKa = 10.58FDD103 pKa = 3.24SVGVISCKK111 pKa = 9.88TLALGINEE119 pKa = 4.4FMLKK123 pKa = 10.25SSWISSFEE131 pKa = 4.23LYY133 pKa = 9.47STDD136 pKa = 3.87VKK138 pKa = 11.15FNLYY142 pKa = 10.26

Molecular weight: 16.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1128

85

403

188.0

21.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.053 ± 0.945

1.684 ± 0.687

5.142 ± 0.519

4.699 ± 0.902

4.876 ± 0.399

6.56 ± 0.638

3.635 ± 0.325

5.496 ± 0.717

5.585 ± 0.958

8.245 ± 0.816

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.039 ± 0.474

4.521 ± 0.725

5.585 ± 0.82

5.142 ± 0.557

6.294 ± 1.42

10.018 ± 0.354

5.674 ± 0.83

4.344 ± 0.488

1.95 ± 0.311

3.457 ± 0.869
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski