Amino acid dipepetide frequency for Enterococcus phage vB_EfaS_LM99

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.575AlaAla: 0.575 ± 0.192
0.164AlaCys: 0.164 ± 0.119
3.453AlaAsp: 3.453 ± 0.577
3.782AlaGlu: 3.782 ± 0.428
3.042AlaPhe: 3.042 ± 0.533
2.96AlaGly: 2.96 ± 0.465
1.151AlaHis: 1.151 ± 0.31
4.768AlaIle: 4.768 ± 0.946
6.084AlaLys: 6.084 ± 0.832
5.097AlaLeu: 5.097 ± 0.88
2.877AlaMet: 2.877 ± 0.565
4.357AlaAsn: 4.357 ± 0.596
1.973AlaPro: 1.973 ± 0.337
1.644AlaGln: 1.644 ± 0.352
1.726AlaArg: 1.726 ± 0.344
2.877AlaSer: 2.877 ± 0.584
4.439AlaThr: 4.439 ± 0.529
4.686AlaVal: 4.686 ± 0.655
0.822AlaTrp: 0.822 ± 0.222
2.549AlaTyr: 2.549 ± 0.442
0.0AlaXaa: 0.0 ± 0.0
Cys
0.329CysAla: 0.329 ± 0.136
0.0CysCys: 0.0 ± 0.0
0.329CysAsp: 0.329 ± 0.173
0.74CysGlu: 0.74 ± 0.255
0.247CysPhe: 0.247 ± 0.128
0.411CysGly: 0.411 ± 0.157
0.164CysHis: 0.164 ± 0.115
0.411CysIle: 0.411 ± 0.209
0.74CysLys: 0.74 ± 0.229
0.658CysLeu: 0.658 ± 0.233
0.0CysMet: 0.0 ± 0.0
0.575CysAsn: 0.575 ± 0.219
0.0CysPro: 0.0 ± 0.0
0.082CysGln: 0.082 ± 0.079
0.247CysArg: 0.247 ± 0.155
0.329CysSer: 0.329 ± 0.165
0.164CysThr: 0.164 ± 0.119
0.493CysVal: 0.493 ± 0.204
0.082CysTrp: 0.082 ± 0.071
0.247CysTyr: 0.247 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
3.617AspAla: 3.617 ± 0.6
0.247AspCys: 0.247 ± 0.113
2.466AspAsp: 2.466 ± 0.559
4.028AspGlu: 4.028 ± 0.607
3.042AspPhe: 3.042 ± 0.536
5.344AspGly: 5.344 ± 0.617
0.74AspHis: 0.74 ± 0.272
4.439AspIle: 4.439 ± 0.708
5.837AspLys: 5.837 ± 0.754
5.097AspLeu: 5.097 ± 0.561
2.22AspMet: 2.22 ± 0.399
4.522AspAsn: 4.522 ± 0.632
2.055AspPro: 2.055 ± 0.45
1.315AspGln: 1.315 ± 0.258
1.809AspArg: 1.809 ± 0.323
2.877AspSer: 2.877 ± 0.429
3.535AspThr: 3.535 ± 0.614
4.11AspVal: 4.11 ± 0.507
0.822AspTrp: 0.822 ± 0.236
2.795AspTyr: 2.795 ± 0.472
0.0AspXaa: 0.0 ± 0.0
Glu
5.179GluAla: 5.179 ± 0.65
0.575GluCys: 0.575 ± 0.227
4.028GluAsp: 4.028 ± 0.873
7.399GluGlu: 7.399 ± 1.611
3.535GluPhe: 3.535 ± 0.574
5.261GluGly: 5.261 ± 0.673
1.233GluHis: 1.233 ± 0.336
4.193GluIle: 4.193 ± 0.61
6.577GluLys: 6.577 ± 0.708
8.468GluLeu: 8.468 ± 1.079
2.22GluMet: 2.22 ± 0.435
3.535GluAsn: 3.535 ± 0.53
2.055GluPro: 2.055 ± 0.566
3.453GluGln: 3.453 ± 0.549
3.453GluArg: 3.453 ± 0.505
3.617GluSer: 3.617 ± 0.505
4.357GluThr: 4.357 ± 0.631
6.166GluVal: 6.166 ± 0.891
1.48GluTrp: 1.48 ± 0.313
3.453GluTyr: 3.453 ± 0.691
0.0GluXaa: 0.0 ± 0.0
Phe
1.726PheAla: 1.726 ± 0.294
0.329PheCys: 0.329 ± 0.144
2.795PheAsp: 2.795 ± 0.564
3.371PheGlu: 3.371 ± 0.596
1.315PhePhe: 1.315 ± 0.413
3.206PheGly: 3.206 ± 0.6
0.411PheHis: 0.411 ± 0.174
4.275PheIle: 4.275 ± 0.665
4.768PheLys: 4.768 ± 0.66
2.384PheLeu: 2.384 ± 0.389
0.987PheMet: 0.987 ± 0.259
3.371PheAsn: 3.371 ± 0.445
0.904PhePro: 0.904 ± 0.229
1.891PheGln: 1.891 ± 0.44
1.644PheArg: 1.644 ± 0.403
1.726PheSer: 1.726 ± 0.358
3.782PheThr: 3.782 ± 0.691
2.96PheVal: 2.96 ± 0.465
0.411PheTrp: 0.411 ± 0.156
1.069PheTyr: 1.069 ± 0.24
0.0PheXaa: 0.0 ± 0.0
Gly
4.11GlyAla: 4.11 ± 1.062
0.411GlyCys: 0.411 ± 0.219
3.453GlyAsp: 3.453 ± 0.503
3.864GlyGlu: 3.864 ± 0.622
3.535GlyPhe: 3.535 ± 0.455
4.604GlyGly: 4.604 ± 1.37
0.658GlyHis: 0.658 ± 0.222
6.001GlyIle: 6.001 ± 0.871
6.166GlyLys: 6.166 ± 0.741
6.495GlyLeu: 6.495 ± 1.145
1.809GlyMet: 1.809 ± 0.382
3.864GlyAsn: 3.864 ± 0.561
0.904GlyPro: 0.904 ± 0.305
1.891GlyGln: 1.891 ± 0.41
2.384GlyArg: 2.384 ± 0.404
3.946GlySer: 3.946 ± 0.57
4.522GlyThr: 4.522 ± 0.819
4.193GlyVal: 4.193 ± 0.751
0.822GlyTrp: 0.822 ± 0.223
2.549GlyTyr: 2.549 ± 0.446
0.0GlyXaa: 0.0 ± 0.0
His
0.987HisAla: 0.987 ± 0.263
0.247HisCys: 0.247 ± 0.16
0.575HisAsp: 0.575 ± 0.249
1.151HisGlu: 1.151 ± 0.342
1.069HisPhe: 1.069 ± 0.329
1.069HisGly: 1.069 ± 0.3
0.329HisHis: 0.329 ± 0.164
0.74HisIle: 0.74 ± 0.215
1.809HisLys: 1.809 ± 0.474
0.658HisLeu: 0.658 ± 0.249
0.411HisMet: 0.411 ± 0.208
0.987HisAsn: 0.987 ± 0.271
0.164HisPro: 0.164 ± 0.131
0.411HisGln: 0.411 ± 0.15
1.233HisArg: 1.233 ± 0.424
0.575HisSer: 0.575 ± 0.247
1.151HisThr: 1.151 ± 0.322
1.069HisVal: 1.069 ± 0.287
0.0HisTrp: 0.0 ± 0.0
0.987HisTyr: 0.987 ± 0.258
0.0HisXaa: 0.0 ± 0.0
Ile
4.193IleAla: 4.193 ± 0.507
0.411IleCys: 0.411 ± 0.211
5.508IleAsp: 5.508 ± 0.579
6.906IleGlu: 6.906 ± 1.031
2.466IlePhe: 2.466 ± 0.587
4.193IleGly: 4.193 ± 0.62
1.069IleHis: 1.069 ± 0.309
4.193IleIle: 4.193 ± 0.507
6.248IleLys: 6.248 ± 0.619
4.85IleLeu: 4.85 ± 0.678
1.562IleMet: 1.562 ± 0.428
4.686IleAsn: 4.686 ± 0.743
2.466IlePro: 2.466 ± 0.374
2.96IleGln: 2.96 ± 0.422
1.644IleArg: 1.644 ± 0.286
4.439IleSer: 4.439 ± 0.538
4.522IleThr: 4.522 ± 0.409
4.275IleVal: 4.275 ± 0.553
0.493IleTrp: 0.493 ± 0.177
1.644IleTyr: 1.644 ± 0.405
0.0IleXaa: 0.0 ± 0.0
Lys
6.577LysAla: 6.577 ± 0.928
0.493LysCys: 0.493 ± 0.208
5.755LysAsp: 5.755 ± 0.609
8.55LysGlu: 8.55 ± 0.979
3.699LysPhe: 3.699 ± 0.589
5.179LysGly: 5.179 ± 0.999
1.644LysHis: 1.644 ± 0.351
4.686LysIle: 4.686 ± 0.648
5.919LysLys: 5.919 ± 0.586
6.906LysLeu: 6.906 ± 0.755
3.371LysMet: 3.371 ± 0.46
5.426LysAsn: 5.426 ± 0.691
3.206LysPro: 3.206 ± 0.531
4.11LysGln: 4.11 ± 0.581
3.699LysArg: 3.699 ± 0.523
4.275LysSer: 4.275 ± 0.85
5.344LysThr: 5.344 ± 0.548
5.261LysVal: 5.261 ± 0.534
1.233LysTrp: 1.233 ± 0.332
3.453LysTyr: 3.453 ± 0.495
0.0LysXaa: 0.0 ± 0.0
Leu
4.193LeuAla: 4.193 ± 0.586
0.575LeuCys: 0.575 ± 0.257
6.495LeuAsp: 6.495 ± 0.639
7.646LeuGlu: 7.646 ± 0.955
2.96LeuPhe: 2.96 ± 0.585
5.179LeuGly: 5.179 ± 1.02
1.233LeuHis: 1.233 ± 0.307
4.439LeuIle: 4.439 ± 0.725
7.892LeuLys: 7.892 ± 0.921
6.659LeuLeu: 6.659 ± 0.852
2.055LeuMet: 2.055 ± 0.359
5.672LeuAsn: 5.672 ± 0.679
2.795LeuPro: 2.795 ± 0.484
4.357LeuGln: 4.357 ± 0.539
2.466LeuArg: 2.466 ± 0.435
4.275LeuSer: 4.275 ± 0.558
4.028LeuThr: 4.028 ± 0.504
5.344LeuVal: 5.344 ± 0.749
0.904LeuTrp: 0.904 ± 0.286
2.713LeuTyr: 2.713 ± 0.574
0.0LeuXaa: 0.0 ± 0.0
Met
1.315MetAla: 1.315 ± 0.333
0.247MetCys: 0.247 ± 0.131
1.891MetAsp: 1.891 ± 0.356
1.891MetGlu: 1.891 ± 0.5
1.48MetPhe: 1.48 ± 0.442
1.973MetGly: 1.973 ± 0.517
0.164MetHis: 0.164 ± 0.109
2.384MetIle: 2.384 ± 0.434
2.877MetLys: 2.877 ± 0.505
1.891MetLeu: 1.891 ± 0.403
0.329MetMet: 0.329 ± 0.185
1.891MetAsn: 1.891 ± 0.435
0.658MetPro: 0.658 ± 0.232
0.822MetGln: 0.822 ± 0.218
1.809MetArg: 1.809 ± 0.376
1.891MetSer: 1.891 ± 0.463
1.809MetThr: 1.809 ± 0.385
1.726MetVal: 1.726 ± 0.416
0.658MetTrp: 0.658 ± 0.308
1.398MetTyr: 1.398 ± 0.402
0.0MetXaa: 0.0 ± 0.0
Asn
4.604AsnAla: 4.604 ± 0.8
0.329AsnCys: 0.329 ± 0.155
2.96AsnAsp: 2.96 ± 0.552
6.001AsnGlu: 6.001 ± 0.778
1.644AsnPhe: 1.644 ± 0.374
5.837AsnGly: 5.837 ± 0.656
0.904AsnHis: 0.904 ± 0.258
4.275AsnIle: 4.275 ± 0.713
5.837AsnLys: 5.837 ± 0.577
4.768AsnLeu: 4.768 ± 0.538
1.973AsnMet: 1.973 ± 0.408
3.453AsnAsn: 3.453 ± 0.518
1.562AsnPro: 1.562 ± 0.342
2.302AsnGln: 2.302 ± 0.34
1.562AsnArg: 1.562 ± 0.304
3.206AsnSer: 3.206 ± 0.549
4.522AsnThr: 4.522 ± 0.584
3.699AsnVal: 3.699 ± 0.586
0.904AsnTrp: 0.904 ± 0.272
3.206AsnTyr: 3.206 ± 0.561
0.0AsnXaa: 0.0 ± 0.0
Pro
1.562ProAla: 1.562 ± 0.358
0.164ProCys: 0.164 ± 0.114
2.137ProAsp: 2.137 ± 0.473
3.124ProGlu: 3.124 ± 0.507
1.233ProPhe: 1.233 ± 0.295
0.164ProGly: 0.164 ± 0.109
0.247ProHis: 0.247 ± 0.142
2.055ProIle: 2.055 ± 0.312
1.891ProLys: 1.891 ± 0.568
2.877ProLeu: 2.877 ± 0.46
0.904ProMet: 0.904 ± 0.238
2.055ProAsn: 2.055 ± 0.463
0.329ProPro: 0.329 ± 0.15
1.233ProGln: 1.233 ± 0.312
0.74ProArg: 0.74 ± 0.244
1.48ProSer: 1.48 ± 0.336
2.055ProThr: 2.055 ± 0.392
1.809ProVal: 1.809 ± 0.346
0.329ProTrp: 0.329 ± 0.132
1.644ProTyr: 1.644 ± 0.454
0.0ProXaa: 0.0 ± 0.0
Gln
2.384GlnAla: 2.384 ± 0.417
0.411GlnCys: 0.411 ± 0.225
1.726GlnAsp: 1.726 ± 0.253
2.137GlnGlu: 2.137 ± 0.471
1.562GlnPhe: 1.562 ± 0.296
2.055GlnGly: 2.055 ± 0.371
0.74GlnHis: 0.74 ± 0.291
2.466GlnIle: 2.466 ± 0.346
2.302GlnLys: 2.302 ± 0.408
3.535GlnLeu: 3.535 ± 0.457
1.315GlnMet: 1.315 ± 0.337
2.055GlnAsn: 2.055 ± 0.466
0.987GlnPro: 0.987 ± 0.373
2.466GlnGln: 2.466 ± 0.444
1.973GlnArg: 1.973 ± 0.447
2.055GlnSer: 2.055 ± 0.43
1.973GlnThr: 1.973 ± 0.317
2.795GlnVal: 2.795 ± 0.396
0.575GlnTrp: 0.575 ± 0.341
2.384GlnTyr: 2.384 ± 0.463
0.0GlnXaa: 0.0 ± 0.0
Arg
1.644ArgAla: 1.644 ± 0.428
0.329ArgCys: 0.329 ± 0.175
2.631ArgAsp: 2.631 ± 0.597
2.22ArgGlu: 2.22 ± 0.452
1.891ArgPhe: 1.891 ± 0.338
1.891ArgGly: 1.891 ± 0.496
0.74ArgHis: 0.74 ± 0.291
2.384ArgIle: 2.384 ± 0.411
2.795ArgLys: 2.795 ± 0.503
3.371ArgLeu: 3.371 ± 0.67
1.069ArgMet: 1.069 ± 0.259
2.22ArgAsn: 2.22 ± 0.43
1.233ArgPro: 1.233 ± 0.326
1.48ArgGln: 1.48 ± 0.342
1.315ArgArg: 1.315 ± 0.294
2.137ArgSer: 2.137 ± 0.439
1.233ArgThr: 1.233 ± 0.298
2.22ArgVal: 2.22 ± 0.424
0.247ArgTrp: 0.247 ± 0.173
1.809ArgTyr: 1.809 ± 0.33
0.0ArgXaa: 0.0 ± 0.0
Ser
3.782SerAla: 3.782 ± 0.819
0.082SerCys: 0.082 ± 0.07
2.795SerAsp: 2.795 ± 0.499
3.042SerGlu: 3.042 ± 0.475
2.549SerPhe: 2.549 ± 0.34
5.097SerGly: 5.097 ± 0.772
1.562SerHis: 1.562 ± 0.323
3.371SerIle: 3.371 ± 0.468
4.275SerLys: 4.275 ± 0.582
3.206SerLeu: 3.206 ± 0.607
1.398SerMet: 1.398 ± 0.244
3.042SerAsn: 3.042 ± 0.533
0.904SerPro: 0.904 ± 0.235
2.384SerGln: 2.384 ± 0.475
1.069SerArg: 1.069 ± 0.382
3.535SerSer: 3.535 ± 0.825
4.11SerThr: 4.11 ± 0.647
2.877SerVal: 2.877 ± 0.518
0.904SerTrp: 0.904 ± 0.231
2.302SerTyr: 2.302 ± 0.529
0.0SerXaa: 0.0 ± 0.0
Thr
4.028ThrAla: 4.028 ± 0.55
0.164ThrCys: 0.164 ± 0.142
3.453ThrAsp: 3.453 ± 0.472
4.357ThrGlu: 4.357 ± 0.572
2.302ThrPhe: 2.302 ± 0.499
4.768ThrGly: 4.768 ± 0.655
1.562ThrHis: 1.562 ± 0.328
4.933ThrIle: 4.933 ± 0.699
6.084ThrLys: 6.084 ± 0.642
5.179ThrLeu: 5.179 ± 0.809
1.562ThrMet: 1.562 ± 0.429
3.699ThrAsn: 3.699 ± 0.701
2.302ThrPro: 2.302 ± 0.436
2.22ThrGln: 2.22 ± 0.449
2.137ThrArg: 2.137 ± 0.418
1.891ThrSer: 1.891 ± 0.337
4.604ThrThr: 4.604 ± 0.866
5.015ThrVal: 5.015 ± 0.597
0.658ThrTrp: 0.658 ± 0.186
1.891ThrTyr: 1.891 ± 0.409
0.0ThrXaa: 0.0 ± 0.0
Val
5.426ValAla: 5.426 ± 0.703
0.329ValCys: 0.329 ± 0.168
4.768ValAsp: 4.768 ± 0.597
5.097ValGlu: 5.097 ± 0.757
3.124ValPhe: 3.124 ± 0.468
4.028ValGly: 4.028 ± 0.724
0.575ValHis: 0.575 ± 0.24
4.439ValIle: 4.439 ± 0.655
5.755ValLys: 5.755 ± 0.831
5.344ValLeu: 5.344 ± 0.725
1.809ValMet: 1.809 ± 0.336
3.782ValAsn: 3.782 ± 0.447
2.137ValPro: 2.137 ± 0.347
1.726ValGln: 1.726 ± 0.42
2.384ValArg: 2.384 ± 0.422
4.604ValSer: 4.604 ± 0.467
3.371ValThr: 3.371 ± 0.562
4.604ValVal: 4.604 ± 0.608
1.151ValTrp: 1.151 ± 0.341
2.549ValTyr: 2.549 ± 0.56
0.0ValXaa: 0.0 ± 0.0
Trp
0.575TrpAla: 0.575 ± 0.217
0.164TrpCys: 0.164 ± 0.116
0.658TrpAsp: 0.658 ± 0.272
1.48TrpGlu: 1.48 ± 0.307
0.904TrpPhe: 0.904 ± 0.257
1.151TrpGly: 1.151 ± 0.288
0.164TrpHis: 0.164 ± 0.107
0.74TrpIle: 0.74 ± 0.277
0.987TrpLys: 0.987 ± 0.25
1.069TrpLeu: 1.069 ± 0.377
0.164TrpMet: 0.164 ± 0.125
0.904TrpAsn: 0.904 ± 0.277
0.0TrpPro: 0.0 ± 0.0
0.164TrpGln: 0.164 ± 0.115
0.575TrpArg: 0.575 ± 0.272
0.575TrpSer: 0.575 ± 0.178
0.658TrpThr: 0.658 ± 0.23
1.315TrpVal: 1.315 ± 0.292
0.411TrpTrp: 0.411 ± 0.17
0.329TrpTyr: 0.329 ± 0.15
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.22TyrAla: 2.22 ± 0.444
0.575TyrCys: 0.575 ± 0.233
3.042TyrAsp: 3.042 ± 0.521
3.453TyrGlu: 3.453 ± 0.626
1.726TyrPhe: 1.726 ± 0.376
1.809TyrGly: 1.809 ± 0.37
0.329TyrHis: 0.329 ± 0.145
3.535TyrIle: 3.535 ± 0.673
3.946TyrLys: 3.946 ± 0.683
3.371TyrLeu: 3.371 ± 0.638
0.987TyrMet: 0.987 ± 0.243
3.535TyrAsn: 3.535 ± 0.625
1.398TyrPro: 1.398 ± 0.372
0.987TyrGln: 0.987 ± 0.292
0.987TyrArg: 0.987 ± 0.37
1.973TyrSer: 1.973 ± 0.579
2.631TyrThr: 2.631 ± 0.635
2.384TyrVal: 2.384 ± 0.395
0.082TyrTrp: 0.082 ± 0.077
1.562TyrTyr: 1.562 ± 0.375
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (12165 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski