Pseudomonas phage YuA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Yuavirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9J572|A9J572_BPPYU Uncharacterized protein gp48 OS=Pseudomonas phage YuA OX=462590 GN=gp48 PE=4 SV=1
MM1 pKa = 7.66ANMSYY6 pKa = 10.38CRR8 pKa = 11.84WEE10 pKa = 3.82NTLNDD15 pKa = 3.8LRR17 pKa = 11.84DD18 pKa = 3.9CAEE21 pKa = 4.33HH22 pKa = 7.31VNDD25 pKa = 4.59PLGGSEE31 pKa = 3.73ARR33 pKa = 11.84ARR35 pKa = 11.84ASLLEE40 pKa = 4.12LAADD44 pKa = 3.64MLEE47 pKa = 4.29EE48 pKa = 4.12VGVTIDD54 pKa = 4.61RR55 pKa = 11.84RR56 pKa = 11.84EE57 pKa = 3.93LDD59 pKa = 3.59EE60 pKa = 5.0ALSNAPGGEE69 pKa = 4.27GG70 pKa = 3.57

Molecular weight: 7.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9J531|A9J531_BPPYU Uncharacterized protein gp25 OS=Pseudomonas phage YuA OX=462590 GN=gp25 PE=4 SV=1
MM1 pKa = 7.76WDD3 pKa = 3.08SLRR6 pKa = 11.84PVIRR10 pKa = 11.84SLDD13 pKa = 3.59PVRR16 pKa = 11.84VEE18 pKa = 3.92NPIVPGTPDD27 pKa = 2.87VNYY30 pKa = 8.9NQGWIEE36 pKa = 4.04LKK38 pKa = 10.43FAEE41 pKa = 4.45RR42 pKa = 11.84WPPRR46 pKa = 11.84GGPLRR51 pKa = 11.84VDD53 pKa = 3.16HH54 pKa = 6.69FTRR57 pKa = 11.84QQRR60 pKa = 11.84TWLTRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84KK68 pKa = 9.59AGGRR72 pKa = 11.84AFLLLKK78 pKa = 10.57VGAIEE83 pKa = 3.82WLLFDD88 pKa = 4.41GAVAAAMLGRR98 pKa = 11.84VPRR101 pKa = 11.84EE102 pKa = 3.31RR103 pKa = 11.84LYY105 pKa = 10.5EE106 pKa = 3.93VCAARR111 pKa = 11.84WTRR114 pKa = 11.84LPRR117 pKa = 11.84TEE119 pKa = 4.98EE120 pKa = 3.49ICKK123 pKa = 10.33CLLYY127 pKa = 10.93

Molecular weight: 14.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

18258

37

940

237.1

26.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.604 ± 0.373

0.969 ± 0.105

5.965 ± 0.174

6.956 ± 0.311

3.681 ± 0.168

8.035 ± 0.245

1.906 ± 0.169

4.387 ± 0.158

4.573 ± 0.273

8.643 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.163 ± 0.123

3.79 ± 0.203

5.192 ± 0.261

3.965 ± 0.23

6.994 ± 0.27

4.847 ± 0.261

5.504 ± 0.231

6.693 ± 0.194

2.054 ± 0.171

3.078 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski