Planctomyces bekefii

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Planctomyces

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3028 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6M982|A0A5C6M982_9PLAN Uncharacterized protein OS=Planctomyces bekefii OX=1653850 GN=E3A20_12880 PE=4 SV=1
RR1 pKa = 8.4LVFTAFDD8 pKa = 3.64SQFRR12 pKa = 11.84RR13 pKa = 11.84QMWTTDD19 pKa = 2.94GTVAGTVPVTNFSGSGLVQYY39 pKa = 11.6ANIVEE44 pKa = 4.67FNNEE48 pKa = 4.04LYY50 pKa = 10.45FAADD54 pKa = 3.53DD55 pKa = 4.22GVNGSEE61 pKa = 5.22LYY63 pKa = 9.75ATDD66 pKa = 3.55GTPAGTRR73 pKa = 11.84LVRR76 pKa = 11.84DD77 pKa = 4.03INPGPDD83 pKa = 2.87SGSNFGNLGPAAILGNMLYY102 pKa = 10.32FAATNGADD110 pKa = 4.71GMEE113 pKa = 4.59LWRR116 pKa = 11.84TDD118 pKa = 2.57GTTAGTQMVSDD129 pKa = 4.62LAAGSEE135 pKa = 4.26GSYY138 pKa = 10.25PSQLVVCNGRR148 pKa = 11.84LLFSAAPASYY158 pKa = 10.46QFALWSTDD166 pKa = 2.69GTSAGTGLVVDD177 pKa = 5.82LSCDD181 pKa = 3.22SLTVVPGPVGQAGDD195 pKa = 4.44DD196 pKa = 3.69EE197 pKa = 5.18SEE199 pKa = 4.37YY200 pKa = 11.39ASGVDD205 pKa = 3.49TTT207 pKa = 4.46

Molecular weight: 21.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6MDA4|A0A5C6MDA4_9PLAN Ribonuclease D OS=Planctomyces bekefii OX=1653850 GN=rnd PE=4 SV=1
MM1 pKa = 7.07VLQRR5 pKa = 11.84VSAVPLPPAVHH16 pKa = 7.6RR17 pKa = 11.84IRR19 pKa = 11.84TVQLRR24 pKa = 11.84LVPAVPLRR32 pKa = 11.84LAVLLTRR39 pKa = 11.84TVQLRR44 pKa = 11.84QVPAVPLRR52 pKa = 11.84QAVLLTRR59 pKa = 11.84IVQLRR64 pKa = 11.84LVPAVQTPAVLLPAVTAVAPSAAKK88 pKa = 10.09AARR91 pKa = 11.84LKK93 pKa = 10.0MRR95 pKa = 11.84ARR97 pKa = 11.84SPSS100 pKa = 3.05

Molecular weight: 10.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3028

0

3028

761673

19

3116

251.5

27.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.885 ± 0.06

1.251 ± 0.02

5.447 ± 0.034

5.92 ± 0.044

3.899 ± 0.031

8.065 ± 0.067

2.036 ± 0.025

4.707 ± 0.043

3.529 ± 0.063

10.553 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.112 ± 0.022

3.167 ± 0.039

4.989 ± 0.042

4.406 ± 0.045

7.026 ± 0.055

6.702 ± 0.045

5.293 ± 0.05

7.151 ± 0.05

1.531 ± 0.021

2.332 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski