Umatilla virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8DP11|G8DP11_9REOV Non-structural protein NS3 OS=Umatilla virus OX=40060 PE=3 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84IVLLAPGDD10 pKa = 3.92IIEE13 pKa = 4.5KK14 pKa = 10.36SEE16 pKa = 4.29PEE18 pKa = 3.91LKK20 pKa = 10.29QRR22 pKa = 11.84GIDD25 pKa = 3.44IKK27 pKa = 11.0IKK29 pKa = 10.15DD30 pKa = 3.4WRR32 pKa = 11.84KK33 pKa = 10.4DD34 pKa = 3.39GGSHH38 pKa = 5.64VPSTEE43 pKa = 3.82RR44 pKa = 11.84EE45 pKa = 3.86NGGTGKK51 pKa = 10.12DD52 pKa = 3.79GEE54 pKa = 4.66SATPGSSGSNGHH66 pKa = 5.88VGRR69 pKa = 11.84DD70 pKa = 3.67GEE72 pKa = 4.27KK73 pKa = 10.48SKK75 pKa = 11.63GNGKK79 pKa = 9.42EE80 pKa = 4.25EE81 pKa = 4.12NDD83 pKa = 3.64SRR85 pKa = 11.84PGDD88 pKa = 3.9SRR90 pKa = 11.84CVRR93 pKa = 11.84TDD95 pKa = 3.1DD96 pKa = 3.73EE97 pKa = 5.9RR98 pKa = 11.84ITKK101 pKa = 8.76TEE103 pKa = 3.88IKK105 pKa = 10.68DD106 pKa = 3.47GDD108 pKa = 3.86GDD110 pKa = 3.92KK111 pKa = 11.51SGDD114 pKa = 3.7KK115 pKa = 10.55QSASAKK121 pKa = 9.7DD122 pKa = 3.57SKK124 pKa = 11.04DD125 pKa = 3.31AEE127 pKa = 4.34GDD129 pKa = 3.43EE130 pKa = 4.56RR131 pKa = 11.84SADD134 pKa = 3.58STVVKK139 pKa = 10.42GKK141 pKa = 10.22KK142 pKa = 9.07IKK144 pKa = 10.3EE145 pKa = 4.17NEE147 pKa = 4.05GTDD150 pKa = 3.62PKK152 pKa = 10.0ITKK155 pKa = 9.59QLAAEE160 pKa = 4.24RR161 pKa = 11.84SIDD164 pKa = 3.46GDD166 pKa = 3.99GPSGSVYY173 pKa = 10.63VLTRR177 pKa = 11.84AVADD181 pKa = 5.07AINQRR186 pKa = 11.84SGKK189 pKa = 9.42QLDD192 pKa = 3.78VLATLSDD199 pKa = 4.03LNGKK203 pKa = 7.65GKK205 pKa = 10.56CYY207 pKa = 10.13QISGGVGKK215 pKa = 10.44IIGLDD220 pKa = 3.08NDD222 pKa = 3.94AVKK225 pKa = 10.47EE226 pKa = 4.0QIDD229 pKa = 3.71ALNEE233 pKa = 3.95LKK235 pKa = 10.66SQYY238 pKa = 10.71DD239 pKa = 3.95GPNVNALLKK248 pKa = 10.02RR249 pKa = 11.84VLRR252 pKa = 11.84IEE254 pKa = 4.34SSSKK258 pKa = 9.97LDD260 pKa = 3.32QMFPKK265 pKa = 10.63NVDD268 pKa = 3.15EE269 pKa = 4.59KK270 pKa = 9.84QTIGSGVVLATNDD283 pKa = 3.71PSFLPQSNALFTCPTGDD300 pKa = 3.9TNWKK304 pKa = 9.14EE305 pKa = 4.27VARR308 pKa = 11.84QATNKK313 pKa = 9.25TSIRR317 pKa = 11.84AYY319 pKa = 8.31TYY321 pKa = 10.84SEE323 pKa = 3.97IGSGSEE329 pKa = 3.44IKK331 pKa = 10.43EE332 pKa = 4.17AFLSLIDD339 pKa = 3.84SLL341 pKa = 4.74

Molecular weight: 36.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8DP11|G8DP11_9REOV Non-structural protein NS3 OS=Umatilla virus OX=40060 PE=3 SV=1
MM1 pKa = 7.51LEE3 pKa = 3.85AAEE6 pKa = 4.22RR7 pKa = 11.84AQEE10 pKa = 3.86RR11 pKa = 11.84SKK13 pKa = 10.55ATEE16 pKa = 3.95SEE18 pKa = 4.47TVRR21 pKa = 11.84EE22 pKa = 4.19DD23 pKa = 3.31SDD25 pKa = 3.93NDD27 pKa = 3.83SMSKK31 pKa = 10.77GLLTEE36 pKa = 4.01TVLRR40 pKa = 11.84DD41 pKa = 3.62TKK43 pKa = 10.63RR44 pKa = 11.84GLGRR48 pKa = 11.84PVTQLTFVDD57 pKa = 4.52EE58 pKa = 4.44LSGGQGFMYY67 pKa = 10.3QQLQDD72 pKa = 3.31KK73 pKa = 10.05AQALNILTNAVTSTTGANDD92 pKa = 3.41TLKK95 pKa = 10.96NEE97 pKa = 4.17KK98 pKa = 10.21AVFGATSDD106 pKa = 3.76ALKK109 pKa = 10.5DD110 pKa = 3.87DD111 pKa = 4.1PFTRR115 pKa = 11.84YY116 pKa = 7.66TKK118 pKa = 10.14QLAYY122 pKa = 7.69EE123 pKa = 4.28TTISQILGQMATKK136 pKa = 9.57RR137 pKa = 11.84RR138 pKa = 11.84KK139 pKa = 8.83MLILDD144 pKa = 3.53VVKK147 pKa = 11.02YY148 pKa = 8.3MLGIILLMLLLWNMIVEE165 pKa = 4.71SIEE168 pKa = 3.85EE169 pKa = 3.88EE170 pKa = 4.38SYY172 pKa = 11.34KK173 pKa = 10.79EE174 pKa = 4.0VLSGMFGKK182 pKa = 10.31NNNDD186 pKa = 3.03TQALFSAARR195 pKa = 11.84AIRR198 pKa = 11.84RR199 pKa = 11.84YY200 pKa = 8.22TSYY203 pKa = 11.69LEE205 pKa = 4.37MISSIAMTFVVRR217 pKa = 11.84KK218 pKa = 10.06IMQLGVKK225 pKa = 10.04LKK227 pKa = 10.63ALEE230 pKa = 3.76RR231 pKa = 11.84DD232 pKa = 3.43KK233 pKa = 11.09IKK235 pKa = 10.89RR236 pKa = 11.84EE237 pKa = 4.07AYY239 pKa = 8.21NKK241 pKa = 8.14TVARR245 pKa = 11.84VSHH248 pKa = 6.46SGICSPSAPTLEE260 pKa = 4.27STEE263 pKa = 4.09RR264 pKa = 3.42

Molecular weight: 29.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6098

264

1299

609.8

69.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.543 ± 0.391

1.23 ± 0.202

5.756 ± 0.35

6.756 ± 0.403

3.673 ± 0.376

5.805 ± 0.368

2.64 ± 0.271

6.642 ± 0.24

5.51 ± 0.675

9.347 ± 0.405

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.935 ± 0.207

4.51 ± 0.352

3.46 ± 0.433

4.182 ± 0.278

6.888 ± 0.345

6.953 ± 0.38

5.953 ± 0.323

6.264 ± 0.284

1.033 ± 0.178

3.919 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski