Nakamurella sp. s14-144

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Nakamurellales; Nakamurellaceae; Nakamurella; unclassified Nakamurella

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2873 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8ZNY2|A0A3G8ZNY2_9ACTN Uncharacterized protein OS=Nakamurella sp. s14-144 OX=1902245 GN=EH165_12510 PE=4 SV=1
MM1 pKa = 7.96PCGQPATFLQFPNVLRR17 pKa = 11.84AGTNVVLGQSVQFQEE32 pKa = 4.4AVNLYY37 pKa = 10.02VDD39 pKa = 4.21AATASAAYY47 pKa = 9.52DD48 pKa = 3.63YY49 pKa = 11.47AVAGLACGDD58 pKa = 3.77GKK60 pKa = 10.68IGSTAVTISEE70 pKa = 4.55PEE72 pKa = 4.1DD73 pKa = 3.19VTATVGGDD81 pKa = 3.23SATTWTVGIEE91 pKa = 4.27GEE93 pKa = 4.36TGVLVSVLSGPLIINFTFIAAAGADD118 pKa = 3.99SSALPDD124 pKa = 4.12PLEE127 pKa = 4.06LAKK130 pKa = 10.72AATAKK135 pKa = 10.68LIAAGGG141 pKa = 3.55

Molecular weight: 13.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8ZRQ0|A0A3G8ZRQ0_9ACTN DUF2631 domain-containing protein OS=Nakamurella sp. s14-144 OX=1902245 GN=EH165_08155 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SKK15 pKa = 8.58THH17 pKa = 5.42GFRR20 pKa = 11.84LRR22 pKa = 11.84MHH24 pKa = 6.26TRR26 pKa = 11.84AGRR29 pKa = 11.84VILAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.87GRR40 pKa = 11.84KK41 pKa = 9.11AISAA45 pKa = 3.87

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2873

0

2873

946164

29

2882

329.3

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.103 ± 0.06

0.748 ± 0.012

5.664 ± 0.037

4.985 ± 0.045

3.039 ± 0.027

8.888 ± 0.041

1.983 ± 0.022

4.648 ± 0.031

2.586 ± 0.031

10.007 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.995 ± 0.018

2.382 ± 0.025

5.479 ± 0.041

3.146 ± 0.027

6.339 ± 0.046

6.503 ± 0.037

6.359 ± 0.06

8.716 ± 0.041

1.411 ± 0.018

2.018 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski