HIV-1 M_97CD.KFE267

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 group M; HIV-1 group M unknown subgroup

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7TQ73|C7TQ73_9HIV1 Envelope glycoprotein gp160 OS=HIV-1 M_97CD.KFE267 OX=576898 GN=gp160 PE=3 SV=1
MM1 pKa = 7.47SPLEE5 pKa = 3.77ISAITGLVVALIIAIVVWSIVIIEE29 pKa = 3.9YY30 pKa = 10.57RR31 pKa = 11.84KK32 pKa = 9.48ILKK35 pKa = 8.7QRR37 pKa = 11.84KK38 pKa = 8.19IDD40 pKa = 3.68RR41 pKa = 11.84LLDD44 pKa = 4.54RR45 pKa = 11.84IRR47 pKa = 11.84DD48 pKa = 3.67RR49 pKa = 11.84AEE51 pKa = 4.09DD52 pKa = 3.62SGNEE56 pKa = 3.89SDD58 pKa = 5.6GDD60 pKa = 4.04AEE62 pKa = 4.12EE63 pKa = 5.07LSALVEE69 pKa = 4.39MGDD72 pKa = 3.84HH73 pKa = 6.47NPWGYY78 pKa = 11.04NVLL81 pKa = 3.79

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7TQ71|C7TQ71_9HIV1 Protein Vpr OS=HIV-1 M_97CD.KFE267 OX=576898 GN=vpr PE=3 SV=1
MM1 pKa = 7.3EE2 pKa = 5.21NRR4 pKa = 11.84WQVMIVWQVDD14 pKa = 3.04RR15 pKa = 11.84MRR17 pKa = 11.84IRR19 pKa = 11.84TWHH22 pKa = 6.19SLVKK26 pKa = 9.26YY27 pKa = 10.15HH28 pKa = 6.5MYY30 pKa = 10.29VSRR33 pKa = 11.84KK34 pKa = 8.98AKK36 pKa = 8.49GWFYY40 pKa = 9.79RR41 pKa = 11.84HH42 pKa = 6.47HH43 pKa = 6.67YY44 pKa = 9.24EE45 pKa = 4.03SRR47 pKa = 11.84HH48 pKa = 5.45PKK50 pKa = 9.69VSSEE54 pKa = 3.57VHH56 pKa = 5.75IPIEE60 pKa = 3.94EE61 pKa = 3.82ARR63 pKa = 11.84LIIRR67 pKa = 11.84TYY69 pKa = 9.86WGLQTEE75 pKa = 4.43EE76 pKa = 5.11KK77 pKa = 10.23DD78 pKa = 3.15WQLGHH83 pKa = 6.04GVSVEE88 pKa = 3.93WSQGRR93 pKa = 11.84YY94 pKa = 6.6RR95 pKa = 11.84TQIDD99 pKa = 4.02PDD101 pKa = 3.72LADD104 pKa = 3.73QLIHH108 pKa = 6.53LQYY111 pKa = 10.4FDD113 pKa = 4.98CFSEE117 pKa = 4.29SAIRR121 pKa = 11.84KK122 pKa = 9.34AILGQIIYY130 pKa = 9.45HH131 pKa = 5.54KK132 pKa = 10.62CEE134 pKa = 3.75YY135 pKa = 9.78QAGHH139 pKa = 6.05KK140 pKa = 9.83QVGSLQYY147 pKa = 10.28LALRR151 pKa = 11.84ALVAPKK157 pKa = 9.87RR158 pKa = 11.84RR159 pKa = 11.84KK160 pKa = 9.52PPLPSVKK167 pKa = 10.27KK168 pKa = 9.35LTEE171 pKa = 4.22DD172 pKa = 3.5RR173 pKa = 11.84WNKK176 pKa = 7.33PQKK179 pKa = 9.47TKK181 pKa = 8.89GHH183 pKa = 6.63RR184 pKa = 11.84EE185 pKa = 3.55NHH187 pKa = 5.05TMNGCC192 pKa = 3.43

Molecular weight: 23.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2878

81

1429

479.7

54.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.324 ± 0.348

1.842 ± 0.364

4.448 ± 0.414

6.845 ± 0.63

2.641 ± 0.34

7.297 ± 0.381

2.363 ± 0.695

6.915 ± 0.671

6.845 ± 0.954

8.478 ± 0.526

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.293 ± 0.202

4.656 ± 0.96

4.899 ± 0.466

5.629 ± 0.558

5.664 ± 1.004

5.108 ± 0.677

5.559 ± 0.814

6.428 ± 0.259

2.988 ± 0.271

2.78 ± 0.478
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski