Bacillus sp. AFS018417

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4515 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A8SIF0|A0A2A8SIF0_9BACI ABC transporter permease OS=Bacillus sp. AFS018417 OX=2033491 GN=CN326_02870 PE=4 SV=1
MM1 pKa = 7.8EE2 pKa = 4.5YY3 pKa = 10.74SGMVSVWFGVSKK15 pKa = 10.77SFEE18 pKa = 4.1NLEE21 pKa = 4.15EE22 pKa = 4.17YY23 pKa = 11.2VDD25 pKa = 3.97IEE27 pKa = 4.24YY28 pKa = 10.32TEE30 pKa = 5.35DD31 pKa = 3.61GDD33 pKa = 5.58SIDD36 pKa = 3.85SRR38 pKa = 11.84FGINFEE44 pKa = 3.89FGYY47 pKa = 10.61YY48 pKa = 10.38DD49 pKa = 3.61EE50 pKa = 6.29DD51 pKa = 4.18NIEE54 pKa = 3.72ICFYY58 pKa = 10.88EE59 pKa = 4.39DD60 pKa = 3.17PKK62 pKa = 11.78NNVDD66 pKa = 5.7DD67 pKa = 4.06ILNDD71 pKa = 3.8FSYY74 pKa = 11.27SEE76 pKa = 4.84LIIPKK81 pKa = 9.76IKK83 pKa = 10.46EE84 pKa = 4.35LINGDD89 pKa = 3.4NLAYY93 pKa = 10.41SINSVIVLYY102 pKa = 10.3DD103 pKa = 3.49FKK105 pKa = 11.49YY106 pKa = 11.13NEE108 pKa = 4.22VKK110 pKa = 10.86SGDD113 pKa = 3.43EE114 pKa = 4.14SEE116 pKa = 4.44NIEE119 pKa = 3.87IKK121 pKa = 10.85FIGTVPYY128 pKa = 10.26KK129 pKa = 10.86

Molecular weight: 15.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A8S8L1|A0A2A8S8L1_9BACI UVR domain-containing protein OS=Bacillus sp. AFS018417 OX=2033491 GN=CN326_15870 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4515

0

4515

1237217

26

1977

274.0

30.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.875 ± 0.041

0.868 ± 0.012

4.63 ± 0.027

7.381 ± 0.045

4.646 ± 0.035

6.731 ± 0.038

2.252 ± 0.021

7.925 ± 0.04

7.141 ± 0.038

9.6 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.871 ± 0.018

4.36 ± 0.027

3.512 ± 0.022

3.992 ± 0.029

3.973 ± 0.027

5.66 ± 0.026

5.574 ± 0.025

7.197 ± 0.033

1.066 ± 0.015

3.744 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski