Hymenobacter sp. PAMC 26554

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter; unclassified Hymenobacter

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4436 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142HJL3|A0A142HJL3_9BACT Pyrroline-5-carboxylate reductase OS=Hymenobacter sp. PAMC 26554 OX=1484116 GN=proC PE=3 SV=1
MM1 pKa = 7.37FLNFFVMANHH11 pKa = 6.19YY12 pKa = 10.03FRR14 pKa = 11.84LNGTDD19 pKa = 4.01FGIGSVQLLLPVTEE33 pKa = 4.42QLLTLKK39 pKa = 9.08ITGSEE44 pKa = 4.19EE45 pKa = 3.56VTEE48 pKa = 5.56AIITDD53 pKa = 3.55EE54 pKa = 4.15AQAFNWILYY63 pKa = 9.38PPMIYY68 pKa = 8.71FTDD71 pKa = 3.38VPYY74 pKa = 11.4ALVNGIKK81 pKa = 9.38TLTISEE87 pKa = 4.49EE88 pKa = 3.9LSDD91 pKa = 4.08NYY93 pKa = 11.1DD94 pKa = 3.05IALYY98 pKa = 11.31LMMHH102 pKa = 7.44DD103 pKa = 5.27DD104 pKa = 3.82LTGTLSITADD114 pKa = 3.63NIITFSGTAQLDD126 pKa = 4.27GADD129 pKa = 3.88LPLEE133 pKa = 4.27IFCVATSS140 pKa = 3.24

Molecular weight: 15.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142HED0|A0A142HED0_9BACT Uncharacterized protein OS=Hymenobacter sp. PAMC 26554 OX=1484116 GN=A0257_09095 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.28LATVFLLSTVKK13 pKa = 10.54FLGGPLAGSSMGLGFWPTLCLTVAGMMTSVFIISGVGRR51 pKa = 11.84AWVRR55 pKa = 11.84HH56 pKa = 4.79RR57 pKa = 11.84QQQRR61 pKa = 11.84RR62 pKa = 11.84LRR64 pKa = 11.84RR65 pKa = 11.84VPVFSKK71 pKa = 10.48RR72 pKa = 11.84SRR74 pKa = 11.84NIIKK78 pKa = 9.39VFKK81 pKa = 9.58RR82 pKa = 11.84FGMGGIAFLTPVLLSPIGGTVVATLLGVPRR112 pKa = 11.84QRR114 pKa = 11.84IMLHH118 pKa = 5.35MFWSAVLWGSVLTLASVRR136 pKa = 11.84IGHH139 pKa = 6.72ILHH142 pKa = 6.72HH143 pKa = 6.58

Molecular weight: 15.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4436

0

4436

1456297

26

2688

328.3

35.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.572 ± 0.047

0.692 ± 0.01

4.837 ± 0.026

4.886 ± 0.041

3.968 ± 0.021

7.842 ± 0.035

2.158 ± 0.02

4.125 ± 0.034

3.629 ± 0.036

11.512 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.753 ± 0.018

3.501 ± 0.041

5.648 ± 0.031

4.628 ± 0.026

6.184 ± 0.035

5.206 ± 0.028

6.165 ± 0.044

7.009 ± 0.027

1.252 ± 0.016

3.432 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski