Anaerobiospirillum thomasii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales; Succinivibrionaceae; Anaerobiospirillum

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2368 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2X0VA45|A0A2X0VA45_9GAMM Flagellin OS=Anaerobiospirillum thomasii OX=179995 GN=flaD_7 PE=3 SV=1
MM1 pKa = 7.66SYY3 pKa = 11.02INIVLDD9 pKa = 4.44GYY11 pKa = 12.01VMDD14 pKa = 5.82LNMDD18 pKa = 3.81KK19 pKa = 10.49LTDD22 pKa = 3.67EE23 pKa = 4.3QVLEE27 pKa = 4.46FKK29 pKa = 10.9DD30 pKa = 3.53AVRR33 pKa = 11.84AYY35 pKa = 10.71DD36 pKa = 3.96KK37 pKa = 11.41DD38 pKa = 3.36PDD40 pKa = 3.67ANYY43 pKa = 10.14IEE45 pKa = 5.34DD46 pKa = 4.69LDD48 pKa = 4.02IFYY51 pKa = 7.92DD52 pKa = 3.77TQINADD58 pKa = 3.8TVQTLRR64 pKa = 11.84GYY66 pKa = 11.57YY67 pKa = 10.39DD68 pKa = 3.76FALDD72 pKa = 3.35AYY74 pKa = 10.65LRR76 pKa = 11.84RR77 pKa = 11.84FDD79 pKa = 6.14DD80 pKa = 4.33DD81 pKa = 3.74GQRR84 pKa = 11.84ITDD87 pKa = 3.96LEE89 pKa = 5.55GDD91 pKa = 3.67DD92 pKa = 3.82EE93 pKa = 5.18VFFEE97 pKa = 5.33EE98 pKa = 4.73IASSAADD105 pKa = 3.39DD106 pKa = 3.97PSFLEE111 pKa = 4.94EE112 pKa = 4.32ISGLDD117 pKa = 4.38LEE119 pKa = 4.86DD120 pKa = 3.88GYY122 pKa = 11.75YY123 pKa = 10.18FVAQRR128 pKa = 11.84VSKK131 pKa = 9.69CTDD134 pKa = 2.94SFIVEE139 pKa = 4.28CDD141 pKa = 3.42EE142 pKa = 5.88EE143 pKa = 5.22IDD145 pKa = 3.95EE146 pKa = 5.19LDD148 pKa = 3.71PFALEE153 pKa = 4.48DD154 pKa = 3.56EE155 pKa = 4.69VAIDD159 pKa = 3.43VCTISEE165 pKa = 4.66LPTVMGRR172 pKa = 11.84EE173 pKa = 4.24INTDD177 pKa = 2.9SYY179 pKa = 11.99NIITNMSYY187 pKa = 10.28KK188 pKa = 10.69GEE190 pKa = 4.12YY191 pKa = 9.49FLNEE195 pKa = 3.7VDD197 pKa = 4.28EE198 pKa = 4.61SPIYY202 pKa = 9.57DD203 pKa = 4.07TIFSTDD209 pKa = 3.03KK210 pKa = 10.9VKK212 pKa = 11.05EE213 pKa = 3.88DD214 pKa = 3.38VLIIFQVVEE223 pKa = 3.92GHH225 pKa = 6.67LSIVYY230 pKa = 9.08TYY232 pKa = 11.12DD233 pKa = 3.21AFSDD237 pKa = 4.2EE238 pKa = 4.69PEE240 pKa = 4.21HH241 pKa = 7.4FYY243 pKa = 11.83DD244 pKa = 5.92HH245 pKa = 7.15IDD247 pKa = 3.37KK248 pKa = 9.82TLVDD252 pKa = 3.61MPIYY256 pKa = 10.19QNNLIQQIYY265 pKa = 10.59LEE267 pKa = 4.54GDD269 pKa = 3.23DD270 pKa = 5.67YY271 pKa = 11.86YY272 pKa = 11.51DD273 pKa = 3.76EE274 pKa = 6.46DD275 pKa = 4.1EE276 pKa = 5.63VDD278 pKa = 5.24DD279 pKa = 4.85EE280 pKa = 6.33DD281 pKa = 6.35YY282 pKa = 11.62DD283 pKa = 5.84DD284 pKa = 5.29EE285 pKa = 6.99DD286 pKa = 4.81SFDD289 pKa = 5.06GEE291 pKa = 5.15DD292 pKa = 3.78FDD294 pKa = 6.15EE295 pKa = 5.66SLDD298 pKa = 4.89DD299 pKa = 6.14DD300 pKa = 5.26YY301 pKa = 12.06EE302 pKa = 6.59DD303 pKa = 6.32DD304 pKa = 4.25DD305 pKa = 6.06DD306 pKa = 5.43EE307 pKa = 4.63YY308 pKa = 11.84ASSRR312 pKa = 11.84KK313 pKa = 9.16KK314 pKa = 10.45DD315 pKa = 3.11RR316 pKa = 11.84RR317 pKa = 11.84RR318 pKa = 11.84YY319 pKa = 9.86EE320 pKa = 3.86GGKK323 pKa = 9.53SDD325 pKa = 5.52KK326 pKa = 10.92EE327 pKa = 3.92DD328 pKa = 4.07DD329 pKa = 3.81NFF331 pKa = 4.78

Molecular weight: 38.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2X0VTE9|A0A2X0VTE9_9GAMM Transposase OS=Anaerobiospirillum thomasii OX=179995 GN=NCTC13093_02409 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTQKK11 pKa = 10.75RR12 pKa = 11.84KK13 pKa = 7.4RR14 pKa = 11.84THH16 pKa = 5.6GFRR19 pKa = 11.84VRR21 pKa = 11.84MRR23 pKa = 11.84TADD26 pKa = 3.26GRR28 pKa = 11.84KK29 pKa = 8.48VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.88GRR39 pKa = 11.84KK40 pKa = 9.09RR41 pKa = 11.84LTTVNGG47 pKa = 3.51

Molecular weight: 5.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2368

0

2368

796788

29

2574

336.5

37.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.971 ± 0.057

1.536 ± 0.02

6.334 ± 0.046

5.772 ± 0.048

4.39 ± 0.034

6.143 ± 0.047

1.954 ± 0.022

7.578 ± 0.042

6.791 ± 0.042

9.795 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.61 ± 0.024

4.97 ± 0.045

3.168 ± 0.032

3.406 ± 0.032

4.386 ± 0.038

7.2 ± 0.039

5.173 ± 0.032

6.497 ± 0.041

0.638 ± 0.013

3.689 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski