Arthrobacter phage BlueFeather

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8R2A8|A0A6G8R2A8_9CAUD Helix-turn-helix DNA binding domain protein OS=Arthrobacter phage BlueFeather OX=2713258 GN=20 PE=4 SV=1
MM1 pKa = 6.83NTHH4 pKa = 7.08HH5 pKa = 7.5DD6 pKa = 3.93GDD8 pKa = 4.41HH9 pKa = 6.57VDD11 pKa = 3.49VDD13 pKa = 4.11PYY15 pKa = 11.05IPTGQCALCLGVPQDD30 pKa = 3.47TTLPEE35 pKa = 4.13EE36 pKa = 4.54RR37 pKa = 11.84EE38 pKa = 4.19GAAPAEE44 pKa = 4.75FIDD47 pKa = 4.77TVGTLL52 pKa = 3.37

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8R2C1|A0A6G8R2C1_9CAUD Tail terminator OS=Arthrobacter phage BlueFeather OX=2713258 GN=7 PE=4 SV=1
MM1 pKa = 7.67AAPSKK6 pKa = 10.68LDD8 pKa = 3.11ARR10 pKa = 11.84GRR12 pKa = 11.84LTALTEE18 pKa = 4.12RR19 pKa = 11.84EE20 pKa = 3.87LAQARR25 pKa = 11.84WDD27 pKa = 3.33EE28 pKa = 4.02ATRR31 pKa = 11.84VKK33 pKa = 10.49DD34 pKa = 4.0AYY36 pKa = 10.86ASMVSKK42 pKa = 10.66RR43 pKa = 11.84RR44 pKa = 11.84STWRR48 pKa = 11.84AAPAGPDD55 pKa = 3.03AAKK58 pKa = 10.49ARR60 pKa = 11.84RR61 pKa = 11.84NLKK64 pKa = 10.01EE65 pKa = 5.16AVDD68 pKa = 3.86QMDD71 pKa = 3.88AASKK75 pKa = 10.82AEE77 pKa = 3.99RR78 pKa = 11.84EE79 pKa = 4.34AWRR82 pKa = 11.84AWHH85 pKa = 6.18RR86 pKa = 11.84LGRR89 pKa = 11.84AEE91 pKa = 4.01TT92 pKa = 3.63

Molecular weight: 10.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

5078

51

560

203.1

21.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.509 ± 0.764

0.571 ± 0.147

6.479 ± 0.512

5.573 ± 0.577

2.816 ± 0.216

8.409 ± 0.385

1.674 ± 0.302

3.998 ± 0.35

3.781 ± 0.656

8.192 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.107 ± 0.231

3.249 ± 0.327

5.396 ± 0.46

3.249 ± 0.255

6.617 ± 0.534

5.553 ± 0.381

7.444 ± 0.647

7.129 ± 0.509

1.772 ± 0.272

2.481 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski