Rhodococcus sp. MEB064

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3865 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D0KD01|A0A0D0KD01_9NOCA Contig_63 whole genome shotgun sequence OS=Rhodococcus sp. MEB064 OX=1587522 GN=RU01_20395 PE=3 SV=1
MM1 pKa = 7.61GGLALLVIAAVVIGGVLWQNSRR23 pKa = 11.84SAPRR27 pKa = 11.84ADD29 pKa = 4.01GYY31 pKa = 11.81GSVQNTAVTTSVDD44 pKa = 3.18PSGVVTLGAPGASRR58 pKa = 11.84TVDD61 pKa = 2.82IFEE64 pKa = 5.6DD65 pKa = 4.18AMCPYY70 pKa = 10.16CAEE73 pKa = 4.39LEE75 pKa = 4.18NTYY78 pKa = 10.56GQEE81 pKa = 3.78LAQKK85 pKa = 9.94IDD87 pKa = 3.81DD88 pKa = 3.82GTVAVRR94 pKa = 11.84YY95 pKa = 10.09HH96 pKa = 6.25MLDD99 pKa = 3.23FLNRR103 pKa = 11.84LSPSGDD109 pKa = 3.12YY110 pKa = 9.25STRR113 pKa = 11.84AVAASQCIAATGDD126 pKa = 3.58GPAFSAFHH134 pKa = 6.79SNLFSPEE141 pKa = 3.88FQPEE145 pKa = 4.2EE146 pKa = 4.24NGSADD151 pKa = 3.35HH152 pKa = 6.71TNAEE156 pKa = 4.33LATAAGDD163 pKa = 3.55AGASDD168 pKa = 4.38EE169 pKa = 4.4AVQCITDD176 pKa = 3.77GAGVATAATNAEE188 pKa = 4.28AGSAALAAAGAQGTPTVIVDD208 pKa = 3.47GAIVDD213 pKa = 4.14TTTDD217 pKa = 3.64TGWVADD223 pKa = 4.39LGG225 pKa = 4.07

Molecular weight: 22.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D0LL20|A0A0D0LL20_9NOCA Contig_16 whole genome shotgun sequence OS=Rhodococcus sp. MEB064 OX=1587522 GN=RU01_08655 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.44GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3865

0

3865

1237203

32

5339

320.1

34.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.656 ± 0.049

0.681 ± 0.009

6.899 ± 0.035

5.218 ± 0.035

3.017 ± 0.023

8.861 ± 0.031

2.097 ± 0.02

4.128 ± 0.021

2.031 ± 0.031

9.651 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.999 ± 0.013

1.907 ± 0.019

5.295 ± 0.027

2.505 ± 0.021

7.24 ± 0.038

6.285 ± 0.022

6.569 ± 0.028

9.577 ± 0.039

1.384 ± 0.016

2.0 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski