Onchocerca ochengi (Filarial nematode worm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Onchocerca

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13353 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P7NF03|A0A3P7NF03_ONCOC Uncharacterized protein (Fragment) OS=Onchocerca ochengi OX=42157 GN=NOO_LOCUS13666 PE=4 SV=1
RR1 pKa = 6.28YY2 pKa = 8.56TMIVLSNVAAPTDD15 pKa = 4.82GIRR18 pKa = 11.84LIQGQVTAYY27 pKa = 9.66IEE29 pKa = 4.28SEE31 pKa = 4.25SAGEE35 pKa = 4.29GEE37 pKa = 4.43LTVSEE42 pKa = 5.32SSVTWISNISGQGFSLTYY60 pKa = 9.77PSIILHH66 pKa = 5.76AVSRR70 pKa = 11.84DD71 pKa = 3.2PSVFPEE77 pKa = 3.73EE78 pKa = 4.69CIYY81 pKa = 11.3VLADD85 pKa = 3.74AKK87 pKa = 11.09GSDD90 pKa = 3.25IGIQSTEE97 pKa = 3.86EE98 pKa = 4.26SVSSVQNVTANGIDD112 pKa = 3.47EE113 pKa = 4.19EE114 pKa = 4.33AAEE117 pKa = 4.54NGCDD121 pKa = 5.72DD122 pKa = 5.96DD123 pKa = 7.56DD124 pKa = 6.62DD125 pKa = 6.87GNDD128 pKa = 3.91DD129 pKa = 5.62DD130 pKa = 6.38DD131 pKa = 5.88DD132 pKa = 4.28KK133 pKa = 12.07AHH135 pKa = 6.42LAIRR139 pKa = 11.84FAPQDD144 pKa = 3.31KK145 pKa = 10.88SILQNIYY152 pKa = 9.69QQMCEE157 pKa = 4.16CQEE160 pKa = 4.15LNPDD164 pKa = 3.79EE165 pKa = 6.04GDD167 pKa = 3.92DD168 pKa = 4.08FSDD171 pKa = 4.58DD172 pKa = 3.57FTMGPDD178 pKa = 3.93DD179 pKa = 5.98DD180 pKa = 4.91EE181 pKa = 5.35EE182 pKa = 6.25DD183 pKa = 4.02YY184 pKa = 11.57EE185 pKa = 5.83GDD187 pKa = 3.7GGEE190 pKa = 4.22GDD192 pKa = 4.49HH193 pKa = 7.18PNILHH198 pKa = 6.13FQNISSDD205 pKa = 3.54MHH207 pKa = 6.67RR208 pKa = 11.84HH209 pKa = 4.29EE210 pKa = 4.75TNGVSSKK217 pKa = 11.37DD218 pKa = 3.37DD219 pKa = 4.29DD220 pKa = 4.41GPP222 pKa = 4.57

Molecular weight: 24.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P7M5E0|A0A3P7M5E0_ONCOC Uncharacterized protein (Fragment) OS=Onchocerca ochengi OX=42157 GN=NOO_LOCUS8734 PE=4 SV=1
MM1 pKa = 7.28AAFRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84FIGRR12 pKa = 11.84RR13 pKa = 11.84IRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84YY19 pKa = 8.65RR20 pKa = 3.34

Molecular weight: 2.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13353

0

13353

4611093

8

7237

345.3

39.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.208 ± 0.018

2.124 ± 0.019

5.534 ± 0.015

6.767 ± 0.023

4.119 ± 0.017

5.012 ± 0.024

2.365 ± 0.01

6.684 ± 0.017

6.276 ± 0.021

9.24 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.524 ± 0.009

5.193 ± 0.016

4.333 ± 0.023

4.179 ± 0.018

5.718 ± 0.018

8.114 ± 0.026

5.522 ± 0.019

5.796 ± 0.015

1.102 ± 0.009

3.153 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski