Nocardioides caeni

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S8NP57|A0A4S8NP57_9ACTN Phenylalanine--tRNA ligase beta subunit OS=Nocardioides caeni OX=574700 GN=pheT PE=3 SV=1
MM1 pKa = 7.43LLVLMALVLSGCGEE15 pKa = 3.95EE16 pKa = 4.33SSGEE20 pKa = 4.01FVGNRR25 pKa = 11.84LDD27 pKa = 3.64QPYY30 pKa = 10.26DD31 pKa = 3.55VPDD34 pKa = 3.55IALTATDD41 pKa = 4.02GSSYY45 pKa = 11.39SLAADD50 pKa = 3.32TDD52 pKa = 3.84KK53 pKa = 11.63ALTLVFFGYY62 pKa = 6.96THH64 pKa = 6.96CPDD67 pKa = 3.46YY68 pKa = 11.31CPLVMNNLSAALNRR82 pKa = 11.84LDD84 pKa = 3.35EE85 pKa = 4.5GEE87 pKa = 4.01RR88 pKa = 11.84SKK90 pKa = 11.66VDD92 pKa = 3.33VVFVTTDD99 pKa = 3.43PSRR102 pKa = 11.84DD103 pKa = 3.73DD104 pKa = 3.47EE105 pKa = 4.61ATLHH109 pKa = 7.25DD110 pKa = 4.36YY111 pKa = 11.26LKK113 pKa = 10.72AYY115 pKa = 9.97DD116 pKa = 3.92AGFIGLTGDD125 pKa = 3.92LDD127 pKa = 5.22DD128 pKa = 6.32IIALGDD134 pKa = 3.77PLHH137 pKa = 7.09VYY139 pKa = 10.56VNDD142 pKa = 4.22GEE144 pKa = 4.21KK145 pKa = 10.72LPTGGYY151 pKa = 10.41DD152 pKa = 3.61LGGHH156 pKa = 5.15STFTLAISSDD166 pKa = 4.0DD167 pKa = 3.56EE168 pKa = 5.88AVALWNQEE176 pKa = 3.78TSSTEE181 pKa = 3.69FANDD185 pKa = 3.37FSILLADD192 pKa = 4.47DD193 pKa = 3.82

Molecular weight: 20.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S8NDQ1|A0A4S8NDQ1_9ACTN Tryptophan synthase beta chain OS=Nocardioides caeni OX=574700 GN=trpB PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3779

0

3779

1241243

33

2496

328.5

35.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.161 ± 0.053

0.724 ± 0.011

6.722 ± 0.034

5.822 ± 0.035

2.787 ± 0.022

9.297 ± 0.038

2.138 ± 0.02

3.759 ± 0.027

1.928 ± 0.028

10.173 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.769 ± 0.015

1.78 ± 0.021

5.62 ± 0.032

2.775 ± 0.022

7.551 ± 0.046

5.202 ± 0.029

6.126 ± 0.034

9.255 ± 0.037

1.516 ± 0.016

1.894 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski