Eggerthella sp. CAG:368

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Eggerthella; environmental samples

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1381 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7BMM0|R7BMM0_9ACTN Ferredoxin OS=Eggerthella sp. CAG:368 OX=1262877 GN=BN629_00706 PE=4 SV=1
MM1 pKa = 7.31SKK3 pKa = 10.38VAIVYY8 pKa = 9.12WSGTGNTEE16 pKa = 3.59AMADD20 pKa = 3.68LFEE23 pKa = 5.03EE24 pKa = 5.3GVTNAGGEE32 pKa = 3.97ASLIQCSNFSSSDD45 pKa = 3.39VSDD48 pKa = 3.72YY49 pKa = 11.68DD50 pKa = 3.93AFAFGCPAMGDD61 pKa = 3.68EE62 pKa = 4.46EE63 pKa = 6.14LEE65 pKa = 4.19DD66 pKa = 3.96TEE68 pKa = 4.62FEE70 pKa = 4.09PMYY73 pKa = 11.02DD74 pKa = 3.52EE75 pKa = 5.7VEE77 pKa = 4.0PALDD81 pKa = 3.62GKK83 pKa = 10.89KK84 pKa = 9.99VVLFGSYY91 pKa = 10.07DD92 pKa = 3.36WNNGEE97 pKa = 3.79WMEE100 pKa = 4.06YY101 pKa = 8.14WEE103 pKa = 4.51QRR105 pKa = 11.84AEE107 pKa = 3.93EE108 pKa = 4.28AGVNVVGTVIAKK120 pKa = 9.51DD121 pKa = 3.88YY122 pKa = 10.56PDD124 pKa = 5.32DD125 pKa = 4.18EE126 pKa = 5.23AAEE129 pKa = 4.14QCKK132 pKa = 9.14QAAASLVV139 pKa = 3.48

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7BQI7|R7BQI7_9ACTN Uncharacterized protein OS=Eggerthella sp. CAG:368 OX=1262877 GN=BN629_00417 PE=4 SV=1
MM1 pKa = 7.36CRR3 pKa = 11.84VHH5 pKa = 6.94AQGCKK10 pKa = 8.82RR11 pKa = 11.84DD12 pKa = 3.33KK13 pKa = 10.37EE14 pKa = 4.45GRR16 pKa = 11.84GGRR19 pKa = 11.84LCTSRR24 pKa = 11.84EE25 pKa = 3.86HH26 pKa = 6.52LRR28 pKa = 11.84GYY30 pKa = 7.76QHH32 pKa = 6.69SGCHH36 pKa = 5.24HH37 pKa = 6.53RR38 pKa = 11.84VLGVRR43 pKa = 11.84VRR45 pKa = 11.84HH46 pKa = 6.2KK47 pKa = 10.65IRR49 pKa = 11.84GEE51 pKa = 3.49YY52 pKa = 10.23HH53 pKa = 6.68RR54 pKa = 11.84IQRR57 pKa = 11.84LFHH60 pKa = 6.86WEE62 pKa = 4.12DD63 pKa = 2.6AWVRR67 pKa = 11.84AFRR70 pKa = 11.84ACQHH74 pKa = 4.72EE75 pKa = 4.65RR76 pKa = 11.84YY77 pKa = 7.7RR78 pKa = 11.84TGWEE82 pKa = 3.47FDD84 pKa = 3.23PRR86 pKa = 11.84AGKK89 pKa = 10.3GDD91 pKa = 3.61RR92 pKa = 11.84VRR94 pKa = 11.84SGWFRR99 pKa = 11.84CSLFIVLSASADD111 pKa = 3.23IRR113 pKa = 11.84PLRR116 pKa = 11.84ICWLVGEE123 pKa = 4.44TRR125 pKa = 11.84TLAMACVSS133 pKa = 3.49

Molecular weight: 15.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1381

0

1381

437682

29

2156

316.9

34.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.271 ± 0.066

1.554 ± 0.027

5.618 ± 0.043

7.125 ± 0.078

4.112 ± 0.049

7.34 ± 0.066

1.882 ± 0.031

6.461 ± 0.058

5.413 ± 0.05

9.226 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.031

3.773 ± 0.042

3.862 ± 0.037

3.169 ± 0.036

4.721 ± 0.053

6.72 ± 0.078

5.511 ± 0.056

7.715 ± 0.053

0.889 ± 0.019

3.04 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski