Amino acid dipepetide frequency for Mycoplasma californicum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.952AlaAla: 2.952 ± 0.135
0.335AlaCys: 0.335 ± 0.036
3.022AlaAsp: 3.022 ± 0.112
3.674AlaGlu: 3.674 ± 0.152
2.717AlaPhe: 2.717 ± 0.109
3.156AlaGly: 3.156 ± 0.167
1.052AlaHis: 1.052 ± 0.071
5.848AlaIle: 5.848 ± 0.187
6.895AlaLys: 6.895 ± 0.248
5.904AlaLeu: 5.904 ± 0.189
1.23AlaMet: 1.23 ± 0.088
3.974AlaAsn: 3.974 ± 0.153
1.5AlaPro: 1.5 ± 0.086
2.956AlaGln: 2.956 ± 0.151
2.361AlaArg: 2.361 ± 0.115
4.135AlaSer: 4.135 ± 0.126
3.365AlaThr: 3.365 ± 0.147
3.0AlaVal: 3.0 ± 0.124
0.396AlaTrp: 0.396 ± 0.04
1.917AlaTyr: 1.917 ± 0.089
0.0AlaXaa: 0.0 ± 0.0
Cys
0.404CysAla: 0.404 ± 0.048
0.035CysCys: 0.035 ± 0.013
0.287CysAsp: 0.287 ± 0.043
0.304CysGlu: 0.304 ± 0.037
0.239CysPhe: 0.239 ± 0.036
0.348CysGly: 0.348 ± 0.045
0.109CysHis: 0.109 ± 0.023
0.313CysIle: 0.313 ± 0.039
0.283CysLys: 0.283 ± 0.037
0.539CysLeu: 0.539 ± 0.051
0.096CysMet: 0.096 ± 0.021
0.226CysAsn: 0.226 ± 0.028
0.187CysPro: 0.187 ± 0.03
0.17CysGln: 0.17 ± 0.031
0.161CysArg: 0.161 ± 0.03
0.439CysSer: 0.439 ± 0.048
0.23CysThr: 0.23 ± 0.03
0.361CysVal: 0.361 ± 0.046
0.061CysTrp: 0.061 ± 0.017
0.148CysTyr: 0.148 ± 0.028
0.0CysXaa: 0.0 ± 0.0
Asp
3.309AspAla: 3.309 ± 0.115
0.187AspCys: 0.187 ± 0.032
2.691AspAsp: 2.691 ± 0.143
4.248AspGlu: 4.248 ± 0.149
3.326AspPhe: 3.326 ± 0.129
2.748AspGly: 2.748 ± 0.125
0.665AspHis: 0.665 ± 0.059
4.587AspIle: 4.587 ± 0.153
5.895AspLys: 5.895 ± 0.205
5.391AspLeu: 5.391 ± 0.188
0.743AspMet: 0.743 ± 0.06
3.491AspAsn: 3.491 ± 0.13
1.761AspPro: 1.761 ± 0.102
1.787AspGln: 1.787 ± 0.09
1.526AspArg: 1.526 ± 0.086
3.487AspSer: 3.487 ± 0.154
2.8AspThr: 2.8 ± 0.126
3.209AspVal: 3.209 ± 0.131
0.587AspTrp: 0.587 ± 0.05
2.465AspTyr: 2.465 ± 0.11
0.0AspXaa: 0.0 ± 0.0
Glu
3.935GluAla: 3.935 ± 0.206
0.235GluCys: 0.235 ± 0.034
2.804GluAsp: 2.804 ± 0.122
4.491GluGlu: 4.491 ± 0.183
3.713GluPhe: 3.713 ± 0.152
2.6GluGly: 2.6 ± 0.134
1.222GluHis: 1.222 ± 0.078
6.704GluIle: 6.704 ± 0.181
7.169GluLys: 7.169 ± 0.252
7.569GluLeu: 7.569 ± 0.215
1.478GluMet: 1.478 ± 0.084
5.139GluAsn: 5.139 ± 0.174
1.6GluPro: 1.6 ± 0.097
2.983GluGln: 2.983 ± 0.131
2.104GluArg: 2.104 ± 0.106
3.156GluSer: 3.156 ± 0.146
3.022GluThr: 3.022 ± 0.123
3.456GluVal: 3.456 ± 0.121
0.587GluTrp: 0.587 ± 0.048
3.022GluTyr: 3.022 ± 0.124
0.0GluXaa: 0.0 ± 0.0
Phe
3.261PheAla: 3.261 ± 0.136
0.335PheCys: 0.335 ± 0.049
3.113PheAsp: 3.113 ± 0.124
3.352PheGlu: 3.352 ± 0.138
2.113PhePhe: 2.113 ± 0.118
2.704PheGly: 2.704 ± 0.143
0.67PheHis: 0.67 ± 0.056
4.587PheIle: 4.587 ± 0.177
4.887PheLys: 4.887 ± 0.149
4.178PheLeu: 4.178 ± 0.169
0.835PheMet: 0.835 ± 0.071
3.996PheAsn: 3.996 ± 0.157
1.074PhePro: 1.074 ± 0.076
1.374PheGln: 1.374 ± 0.07
1.509PheArg: 1.509 ± 0.072
3.761PheSer: 3.761 ± 0.145
2.513PheThr: 2.513 ± 0.107
3.061PheVal: 3.061 ± 0.131
0.565PheTrp: 0.565 ± 0.051
1.9PheTyr: 1.9 ± 0.108
0.0PheXaa: 0.0 ± 0.0
Gly
3.03GlyAla: 3.03 ± 0.147
0.313GlyCys: 0.313 ± 0.04
2.656GlyAsp: 2.656 ± 0.113
2.813GlyGlu: 2.813 ± 0.148
2.648GlyPhe: 2.648 ± 0.127
2.952GlyGly: 2.952 ± 0.159
0.961GlyHis: 0.961 ± 0.073
4.356GlyIle: 4.356 ± 0.151
4.435GlyLys: 4.435 ± 0.158
4.43GlyLeu: 4.43 ± 0.17
1.052GlyMet: 1.052 ± 0.081
2.456GlyAsn: 2.456 ± 0.135
1.091GlyPro: 1.091 ± 0.087
1.996GlyGln: 1.996 ± 0.115
1.691GlyArg: 1.691 ± 0.104
3.252GlySer: 3.252 ± 0.158
2.578GlyThr: 2.578 ± 0.119
3.274GlyVal: 3.274 ± 0.147
0.565GlyTrp: 0.565 ± 0.046
2.13GlyTyr: 2.13 ± 0.088
0.0GlyXaa: 0.0 ± 0.0
His
0.926HisAla: 0.926 ± 0.071
0.109HisCys: 0.109 ± 0.024
0.77HisAsp: 0.77 ± 0.062
1.026HisGlu: 1.026 ± 0.065
0.961HisPhe: 0.961 ± 0.072
0.991HisGly: 0.991 ± 0.089
0.33HisHis: 0.33 ± 0.035
1.374HisIle: 1.374 ± 0.074
1.674HisLys: 1.674 ± 0.09
1.452HisLeu: 1.452 ± 0.075
0.23HisMet: 0.23 ± 0.026
1.23HisAsn: 1.23 ± 0.082
0.739HisPro: 0.739 ± 0.06
0.587HisGln: 0.587 ± 0.043
0.513HisArg: 0.513 ± 0.056
1.143HisSer: 1.143 ± 0.074
0.874HisThr: 0.874 ± 0.071
0.787HisVal: 0.787 ± 0.06
0.196HisTrp: 0.196 ± 0.032
0.756HisTyr: 0.756 ± 0.057
0.0HisXaa: 0.0 ± 0.0
Ile
6.13IleAla: 6.13 ± 0.183
0.535IleCys: 0.535 ± 0.048
5.891IleAsp: 5.891 ± 0.164
6.582IleGlu: 6.582 ± 0.242
4.339IlePhe: 4.339 ± 0.166
4.243IleGly: 4.243 ± 0.18
1.352IleHis: 1.352 ± 0.074
7.656IleIle: 7.656 ± 0.209
9.026IleLys: 9.026 ± 0.238
7.761IleLeu: 7.761 ± 0.242
1.604IleMet: 1.604 ± 0.099
6.874IleAsn: 6.874 ± 0.172
2.769IlePro: 2.769 ± 0.11
3.022IleGln: 3.022 ± 0.133
2.439IleArg: 2.439 ± 0.095
6.878IleSer: 6.878 ± 0.167
4.822IleThr: 4.822 ± 0.138
5.195IleVal: 5.195 ± 0.159
0.7IleTrp: 0.7 ± 0.053
3.304IleTyr: 3.304 ± 0.122
0.0IleXaa: 0.0 ± 0.0
Lys
5.404LysAla: 5.404 ± 0.257
0.287LysCys: 0.287 ± 0.034
5.678LysAsp: 5.678 ± 0.203
7.03LysGlu: 7.03 ± 0.268
4.435LysPhe: 4.435 ± 0.147
3.643LysGly: 3.643 ± 0.146
1.717LysHis: 1.717 ± 0.092
10.408LysIle: 10.408 ± 0.281
8.665LysLys: 8.665 ± 0.236
8.613LysLeu: 8.613 ± 0.244
2.452LysMet: 2.452 ± 0.105
8.339LysAsn: 8.339 ± 0.233
2.996LysPro: 2.996 ± 0.152
3.9LysGln: 3.9 ± 0.192
3.035LysArg: 3.035 ± 0.128
6.004LysSer: 6.004 ± 0.201
5.948LysThr: 5.948 ± 0.182
5.109LysVal: 5.109 ± 0.144
0.996LysTrp: 0.996 ± 0.068
4.548LysTyr: 4.548 ± 0.171
0.0LysXaa: 0.0 ± 0.0
Leu
6.261LeuAla: 6.261 ± 0.177
0.53LeuCys: 0.53 ± 0.048
5.278LeuAsp: 5.278 ± 0.19
6.009LeuGlu: 6.009 ± 0.217
4.382LeuPhe: 4.382 ± 0.192
4.6LeuGly: 4.6 ± 0.131
1.57LeuHis: 1.57 ± 0.089
8.491LeuIle: 8.491 ± 0.227
9.256LeuLys: 9.256 ± 0.234
7.769LeuLeu: 7.769 ± 0.229
1.53LeuMet: 1.53 ± 0.084
6.904LeuAsn: 6.904 ± 0.202
2.7LeuPro: 2.7 ± 0.126
2.869LeuGln: 2.869 ± 0.129
2.991LeuArg: 2.991 ± 0.124
6.817LeuSer: 6.817 ± 0.166
4.865LeuThr: 4.865 ± 0.14
5.456LeuVal: 5.456 ± 0.157
0.774LeuTrp: 0.774 ± 0.057
2.822LeuTyr: 2.822 ± 0.122
0.0LeuXaa: 0.0 ± 0.0
Met
1.191MetAla: 1.191 ± 0.086
0.117MetCys: 0.117 ± 0.021
0.917MetAsp: 0.917 ± 0.07
0.883MetGlu: 0.883 ± 0.07
1.022MetPhe: 1.022 ± 0.078
0.839MetGly: 0.839 ± 0.067
0.304MetHis: 0.304 ± 0.042
1.548MetIle: 1.548 ± 0.09
1.739MetLys: 1.739 ± 0.097
1.639MetLeu: 1.639 ± 0.099
0.439MetMet: 0.439 ± 0.048
1.422MetAsn: 1.422 ± 0.081
0.761MetPro: 0.761 ± 0.059
0.883MetGln: 0.883 ± 0.063
0.761MetArg: 0.761 ± 0.061
1.496MetSer: 1.496 ± 0.087
0.787MetThr: 0.787 ± 0.06
0.991MetVal: 0.991 ± 0.076
0.13MetTrp: 0.13 ± 0.024
0.583MetTyr: 0.583 ± 0.051
0.0MetXaa: 0.0 ± 0.0
Asn
3.904AsnAla: 3.904 ± 0.168
0.257AsnCys: 0.257 ± 0.036
3.848AsnAsp: 3.848 ± 0.149
5.017AsnGlu: 5.017 ± 0.197
3.478AsnPhe: 3.478 ± 0.138
3.396AsnGly: 3.396 ± 0.143
1.104AsnHis: 1.104 ± 0.078
6.643AsnIle: 6.643 ± 0.182
7.395AsnLys: 7.395 ± 0.198
6.565AsnLeu: 6.565 ± 0.208
1.091AsnMet: 1.091 ± 0.068
5.452AsnAsn: 5.452 ± 0.207
2.743AsnPro: 2.743 ± 0.113
2.804AsnGln: 2.804 ± 0.143
2.048AsnArg: 2.048 ± 0.095
5.195AsnSer: 5.195 ± 0.215
3.548AsnThr: 3.548 ± 0.15
3.678AsnVal: 3.678 ± 0.122
0.67AsnTrp: 0.67 ± 0.058
2.791AsnTyr: 2.791 ± 0.105
0.0AsnXaa: 0.0 ± 0.0
Pro
1.513ProAla: 1.513 ± 0.102
0.157ProCys: 0.157 ± 0.027
1.717ProAsp: 1.717 ± 0.09
2.374ProGlu: 2.374 ± 0.107
1.409ProPhe: 1.409 ± 0.074
1.487ProGly: 1.487 ± 0.097
0.57ProHis: 0.57 ± 0.05
2.478ProIle: 2.478 ± 0.089
2.774ProLys: 2.774 ± 0.145
2.548ProLeu: 2.548 ± 0.111
0.452ProMet: 0.452 ± 0.048
1.983ProAsn: 1.983 ± 0.101
0.535ProPro: 0.535 ± 0.064
1.278ProGln: 1.278 ± 0.085
0.904ProArg: 0.904 ± 0.062
1.783ProSer: 1.783 ± 0.091
1.822ProThr: 1.822 ± 0.09
1.861ProVal: 1.861 ± 0.107
0.352ProTrp: 0.352 ± 0.041
1.261ProTyr: 1.261 ± 0.08
0.0ProXaa: 0.0 ± 0.0
Gln
2.63GlnAla: 2.63 ± 0.155
0.148GlnCys: 0.148 ± 0.025
1.678GlnAsp: 1.678 ± 0.101
2.278GlnGlu: 2.278 ± 0.113
1.713GlnPhe: 1.713 ± 0.09
1.556GlnGly: 1.556 ± 0.074
0.661GlnHis: 0.661 ± 0.057
3.687GlnIle: 3.687 ± 0.134
4.691GlnLys: 4.691 ± 0.196
3.122GlnLeu: 3.122 ± 0.17
0.674GlnMet: 0.674 ± 0.061
3.339GlnAsn: 3.339 ± 0.151
1.03GlnPro: 1.03 ± 0.075
1.67GlnGln: 1.67 ± 0.095
1.283GlnArg: 1.283 ± 0.081
2.226GlnSer: 2.226 ± 0.112
2.191GlnThr: 2.191 ± 0.11
1.822GlnVal: 1.822 ± 0.096
0.387GlnTrp: 0.387 ± 0.036
1.656GlnTyr: 1.656 ± 0.079
0.0GlnXaa: 0.0 ± 0.0
Arg
1.948ArgAla: 1.948 ± 0.106
0.135ArgCys: 0.135 ± 0.028
1.73ArgAsp: 1.73 ± 0.092
2.378ArgGlu: 2.378 ± 0.116
1.678ArgPhe: 1.678 ± 0.095
1.565ArgGly: 1.565 ± 0.103
0.583ArgHis: 0.583 ± 0.053
2.8ArgIle: 2.8 ± 0.116
2.943ArgLys: 2.943 ± 0.12
2.878ArgLeu: 2.878 ± 0.119
0.726ArgMet: 0.726 ± 0.057
2.0ArgAsn: 2.0 ± 0.094
0.883ArgPro: 0.883 ± 0.068
1.226ArgGln: 1.226 ± 0.067
1.4ArgArg: 1.4 ± 0.096
2.004ArgSer: 2.004 ± 0.095
1.383ArgThr: 1.383 ± 0.077
2.204ArgVal: 2.204 ± 0.107
0.33ArgTrp: 0.33 ± 0.038
1.339ArgTyr: 1.339 ± 0.083
0.0ArgXaa: 0.0 ± 0.0
Ser
3.804SerAla: 3.804 ± 0.162
0.474SerCys: 0.474 ± 0.05
3.652SerAsp: 3.652 ± 0.135
4.148SerGlu: 4.148 ± 0.14
3.604SerPhe: 3.604 ± 0.134
3.587SerGly: 3.587 ± 0.156
1.122SerHis: 1.122 ± 0.072
5.965SerIle: 5.965 ± 0.185
6.317SerLys: 6.317 ± 0.206
6.648SerLeu: 6.648 ± 0.184
1.139SerMet: 1.139 ± 0.088
4.478SerAsn: 4.478 ± 0.161
1.77SerPro: 1.77 ± 0.098
2.965SerGln: 2.965 ± 0.117
2.091SerArg: 2.091 ± 0.101
4.922SerSer: 4.922 ± 0.167
3.774SerThr: 3.774 ± 0.129
4.022SerVal: 4.022 ± 0.117
0.67SerTrp: 0.67 ± 0.043
2.461SerTyr: 2.461 ± 0.108
0.0SerXaa: 0.0 ± 0.0
Thr
2.665ThrAla: 2.665 ± 0.108
0.204ThrCys: 0.204 ± 0.031
2.796ThrAsp: 2.796 ± 0.134
3.1ThrGlu: 3.1 ± 0.134
2.561ThrPhe: 2.561 ± 0.107
2.73ThrGly: 2.73 ± 0.151
1.178ThrHis: 1.178 ± 0.08
4.722ThrIle: 4.722 ± 0.142
5.43ThrLys: 5.43 ± 0.192
5.048ThrLeu: 5.048 ± 0.153
0.852ThrMet: 0.852 ± 0.064
3.404ThrAsn: 3.404 ± 0.123
2.135ThrPro: 2.135 ± 0.094
2.369ThrGln: 2.369 ± 0.122
1.896ThrArg: 1.896 ± 0.091
3.422ThrSer: 3.422 ± 0.138
3.03ThrThr: 3.03 ± 0.136
2.674ThrVal: 2.674 ± 0.121
0.452ThrTrp: 0.452 ± 0.05
1.943ThrTyr: 1.943 ± 0.103
0.0ThrXaa: 0.0 ± 0.0
Val
4.061ValAla: 4.061 ± 0.138
0.326ValCys: 0.326 ± 0.043
3.552ValAsp: 3.552 ± 0.142
4.043ValGlu: 4.043 ± 0.162
2.791ValPhe: 2.791 ± 0.139
3.126ValGly: 3.126 ± 0.151
0.761ValHis: 0.761 ± 0.065
4.935ValIle: 4.935 ± 0.177
5.369ValLys: 5.369 ± 0.162
5.056ValLeu: 5.056 ± 0.158
0.974ValMet: 0.974 ± 0.079
3.7ValAsn: 3.7 ± 0.118
1.617ValPro: 1.617 ± 0.102
1.887ValGln: 1.887 ± 0.087
1.713ValArg: 1.713 ± 0.097
3.909ValSer: 3.909 ± 0.133
2.369ValThr: 2.369 ± 0.115
3.843ValVal: 3.843 ± 0.151
0.513ValTrp: 0.513 ± 0.054
2.143ValTyr: 2.143 ± 0.109
0.0ValXaa: 0.0 ± 0.0
Trp
0.609TrpAla: 0.609 ± 0.056
0.061TrpCys: 0.061 ± 0.017
0.517TrpAsp: 0.517 ± 0.039
0.683TrpGlu: 0.683 ± 0.05
0.57TrpPhe: 0.57 ± 0.054
0.426TrpGly: 0.426 ± 0.045
0.1TrpHis: 0.1 ± 0.021
0.87TrpIle: 0.87 ± 0.059
0.804TrpLys: 0.804 ± 0.066
0.913TrpLeu: 0.913 ± 0.06
0.27TrpMet: 0.27 ± 0.035
0.665TrpAsn: 0.665 ± 0.05
0.261TrpPro: 0.261 ± 0.035
0.313TrpGln: 0.313 ± 0.037
0.335TrpArg: 0.335 ± 0.04
0.591TrpSer: 0.591 ± 0.057
0.483TrpThr: 0.483 ± 0.044
0.596TrpVal: 0.596 ± 0.054
0.126TrpTrp: 0.126 ± 0.02
0.409TrpTyr: 0.409 ± 0.041
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.452TyrAla: 2.452 ± 0.095
0.161TyrCys: 0.161 ± 0.025
2.304TyrAsp: 2.304 ± 0.119
2.556TyrGlu: 2.556 ± 0.112
2.087TyrPhe: 2.087 ± 0.118
1.983TyrGly: 1.983 ± 0.091
0.639TyrHis: 0.639 ± 0.063
3.152TyrIle: 3.152 ± 0.135
3.556TyrLys: 3.556 ± 0.136
3.852TyrLeu: 3.852 ± 0.13
0.578TyrMet: 0.578 ± 0.052
2.556TyrAsn: 2.556 ± 0.108
1.104TyrPro: 1.104 ± 0.073
1.409TyrGln: 1.409 ± 0.078
1.374TyrArg: 1.374 ± 0.077
2.991TyrSer: 2.991 ± 0.122
2.209TyrThr: 2.209 ± 0.092
2.091TyrVal: 2.091 ± 0.097
0.543TyrTrp: 0.543 ± 0.049
1.417TyrTyr: 1.417 ± 0.098
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 610 proteins (230008 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski