endosymbiont of Pachyrhynchus infernalis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Candidatus Nardonella

Average proteome isoelectric point is 8.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5THM5|A0A2Z5THM5_9GAMM 50S ribosomal protein L25 OS=endosymbiont of Pachyrhynchus infernalis OX=1971488 GN=rplY PE=4 SV=1
MM1 pKa = 7.72NDD3 pKa = 3.23NKK5 pKa = 10.96DD6 pKa = 3.42ILEE9 pKa = 4.13EE10 pKa = 4.47EE11 pKa = 3.71IDD13 pKa = 4.05INKK16 pKa = 9.82KK17 pKa = 9.95DD18 pKa = 5.07DD19 pKa = 3.85KK20 pKa = 11.66NLDD23 pKa = 3.58NLINEE28 pKa = 4.81YY29 pKa = 10.65INNINNLNNVIEE41 pKa = 4.22NYY43 pKa = 10.26KK44 pKa = 10.17IEE46 pKa = 4.63LDD48 pKa = 3.56NLKK51 pKa = 10.38KK52 pKa = 10.77EE53 pKa = 4.45KK54 pKa = 9.96IDD56 pKa = 3.71NEE58 pKa = 3.96LRR60 pKa = 11.84YY61 pKa = 9.35QAEE64 pKa = 4.15LSNIDD69 pKa = 3.55KK70 pKa = 10.56RR71 pKa = 11.84NKK73 pKa = 9.72IEE75 pKa = 3.57IEE77 pKa = 4.11KK78 pKa = 9.98IYY80 pKa = 10.77KK81 pKa = 10.19FSLEE85 pKa = 3.93NFMSEE90 pKa = 4.6LIIVIDD96 pKa = 3.64NLEE99 pKa = 3.73RR100 pKa = 11.84AINVNVDD107 pKa = 4.2KK108 pKa = 11.24INDD111 pKa = 4.26DD112 pKa = 3.71IIEE115 pKa = 4.32GLNLTLKK122 pKa = 10.77SLVKK126 pKa = 9.34ITNKK130 pKa = 10.19FGLSSIDD137 pKa = 3.98SINVPFDD144 pKa = 3.65PSIHH148 pKa = 5.33QAILTTFNKK157 pKa = 9.98KK158 pKa = 10.19YY159 pKa = 9.6EE160 pKa = 4.18PNYY163 pKa = 10.51VIDD166 pKa = 5.45IIQKK170 pKa = 10.26GYY172 pKa = 10.26ILNEE176 pKa = 3.79RR177 pKa = 11.84LIRR180 pKa = 11.84PAMVQVSCNSEE191 pKa = 3.85NNIEE195 pKa = 4.05KK196 pKa = 10.72NMNIDD201 pKa = 3.52NKK203 pKa = 11.11LEE205 pKa = 3.87

Molecular weight: 23.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5THG2|A0A2Z5THG2_9GAMM Beta sliding clamp OS=endosymbiont of Pachyrhynchus infernalis OX=1971488 GN=dnaN PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84IKK11 pKa = 10.51RR12 pKa = 11.84IRR14 pKa = 11.84KK15 pKa = 7.3IGFRR19 pKa = 11.84IRR21 pKa = 11.84MSTKK25 pKa = 8.3SGRR28 pKa = 11.84RR29 pKa = 11.84ILSNRR34 pKa = 11.84RR35 pKa = 11.84NKK37 pKa = 9.33KK38 pKa = 9.46RR39 pKa = 11.84KK40 pKa = 8.72NLILIKK46 pKa = 10.58YY47 pKa = 9.28KK48 pKa = 10.79YY49 pKa = 10.36

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

212

0

212

67878

37

1357

320.2

37.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.777 ± 0.112

1.086 ± 0.05

4.398 ± 0.119

4.467 ± 0.144

5.475 ± 0.155

4.159 ± 0.125

1.073 ± 0.047

16.272 ± 0.215

12.343 ± 0.198

9.605 ± 0.135

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.354 ± 0.051

13.406 ± 0.274

2.063 ± 0.07

1.252 ± 0.062

2.41 ± 0.089

6.909 ± 0.124

2.914 ± 0.079

3.131 ± 0.116

0.569 ± 0.049

5.339 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski