Ciceribacter sp. F8825

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Ciceribacter; unclassified Ciceribacter

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4841 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2T1I1|A0A4Q2T1I1_9RHIZ Uncharacterized protein OS=Ciceribacter sp. F8825 OX=2509717 GN=EUU22_12290 PE=4 SV=1
MM1 pKa = 7.26ARR3 pKa = 11.84SVEE6 pKa = 4.11NALGIPLYY14 pKa = 11.03YY15 pKa = 10.51SGTSTAWFSATGSGPSLYY33 pKa = 9.26GTAGNDD39 pKa = 4.12SIWGDD44 pKa = 3.38SSVNVTMYY52 pKa = 10.91GGTGDD57 pKa = 4.88DD58 pKa = 3.2IYY60 pKa = 11.69YY61 pKa = 10.45LYY63 pKa = 10.06STINRR68 pKa = 11.84AVEE71 pKa = 3.96APGEE75 pKa = 4.44GIDD78 pKa = 5.03TIKK81 pKa = 9.65TWMSYY86 pKa = 7.28TLPEE90 pKa = 4.05NFEE93 pKa = 4.33NLSVTGDD100 pKa = 3.24VRR102 pKa = 11.84HH103 pKa = 6.52AFGNDD108 pKa = 2.87LDD110 pKa = 4.91NIISGSSNRR119 pKa = 11.84QTFDD123 pKa = 2.73GGAGNDD129 pKa = 3.49VLTGGGGADD138 pKa = 3.24IFIVDD143 pKa = 4.52KK144 pKa = 11.6GNGSDD149 pKa = 5.15LITDD153 pKa = 4.27FRR155 pKa = 11.84ADD157 pKa = 3.28DD158 pKa = 4.18TIRR161 pKa = 11.84LEE163 pKa = 4.9GYY165 pKa = 10.25AFTSFAEE172 pKa = 4.59VSSHH176 pKa = 5.65LTQEE180 pKa = 4.34GTDD183 pKa = 3.36VRR185 pKa = 11.84LDD187 pKa = 3.69LGDD190 pKa = 4.2GEE192 pKa = 4.99SLVFADD198 pKa = 4.62VAVGDD203 pKa = 4.42LGANQFRR210 pKa = 11.84LSLDD214 pKa = 3.47RR215 pKa = 11.84SHH217 pKa = 7.07LTQTFSDD224 pKa = 4.18DD225 pKa = 4.16FNTLSLNDD233 pKa = 4.02GSGGTWDD240 pKa = 4.2PKK242 pKa = 10.55FWWAPEE248 pKa = 4.16KK249 pKa = 10.86GSSLAGNGEE258 pKa = 4.33LQWYY262 pKa = 9.12INPAYY267 pKa = 10.03EE268 pKa = 4.45GTSEE272 pKa = 4.11VNPFSVEE279 pKa = 3.96NGVLTITAEE288 pKa = 3.89QAPPSIQSEE297 pKa = 4.36IEE299 pKa = 4.37GYY301 pKa = 10.67DD302 pKa = 3.46YY303 pKa = 11.2TSGMLTTYY311 pKa = 10.96SSFAQTYY318 pKa = 8.7GYY320 pKa = 10.77FEE322 pKa = 4.72IRR324 pKa = 11.84ADD326 pKa = 3.68MPDD329 pKa = 3.52EE330 pKa = 4.25QGTWPAFWLLPADD343 pKa = 4.39GSWPPEE349 pKa = 3.76LDD351 pKa = 3.52VVEE354 pKa = 5.06MRR356 pKa = 11.84GQDD359 pKa = 3.54PNTVHH364 pKa = 6.68VSVHH368 pKa = 5.61SNEE371 pKa = 3.98TGQRR375 pKa = 11.84TTTSTAVQVSGTEE388 pKa = 4.0GFHH391 pKa = 6.78NYY393 pKa = 8.39GVLWTEE399 pKa = 4.54EE400 pKa = 4.13EE401 pKa = 4.36IVWYY405 pKa = 10.18FDD407 pKa = 3.44DD408 pKa = 4.07VAVARR413 pKa = 11.84ADD415 pKa = 3.96TPADD419 pKa = 3.41MHH421 pKa = 7.3DD422 pKa = 3.79PMYY425 pKa = 10.22MIVNLAVGGSAGTPADD441 pKa = 3.63GLAGGAEE448 pKa = 3.94MQIDD452 pKa = 4.22YY453 pKa = 10.83IKK455 pKa = 10.56AYY457 pKa = 10.71SLDD460 pKa = 3.73DD461 pKa = 3.75VMPAQQQQTSEE472 pKa = 3.9WLLL475 pKa = 3.6

Molecular weight: 51.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1RPQ3|A0A4V1RPQ3_9RHIZ Type II toxin-antitoxin system HicB family antitoxin OS=Ciceribacter sp. F8825 OX=2509717 GN=EUU22_19010 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATNGGRR28 pKa = 11.84KK29 pKa = 8.93VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4841

0

4841

1479523

27

2833

305.6

33.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.12 ± 0.053

0.837 ± 0.012

5.663 ± 0.028

6.078 ± 0.033

3.892 ± 0.024

8.485 ± 0.036

1.998 ± 0.018

5.37 ± 0.025

3.483 ± 0.03

9.996 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.55 ± 0.015

2.624 ± 0.021

4.957 ± 0.024

2.857 ± 0.022

7.022 ± 0.036

5.537 ± 0.026

5.337 ± 0.024

7.628 ± 0.026

1.268 ± 0.014

2.299 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski