Hibiscus syriacus (Rose of Sharon)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Hibiscus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82460 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A2X3B2|A0A6A2X3B2_HIBSY Patatin OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00117002pilonHSYRG00052 PE=3 SV=1
MM1 pKa = 6.87EE2 pKa = 5.97TITASFRR9 pKa = 11.84KK10 pKa = 9.58ISLNHH15 pKa = 6.15KK16 pKa = 9.73GGPEE20 pKa = 4.04EE21 pKa = 4.2EE22 pKa = 5.42DD23 pKa = 3.59GDD25 pKa = 3.99NKK27 pKa = 10.38IPNGAVSEE35 pKa = 4.37LSSHH39 pKa = 6.34VSLPYY44 pKa = 10.27YY45 pKa = 10.66LKK47 pKa = 10.23QQCLDD52 pKa = 3.69LKK54 pKa = 10.85AAEE57 pKa = 4.22IQCKK61 pKa = 7.9NRR63 pKa = 11.84RR64 pKa = 11.84DD65 pKa = 3.97GVEE68 pKa = 3.8PADD71 pKa = 4.73DD72 pKa = 3.75PRR74 pKa = 11.84AEE76 pKa = 4.12YY77 pKa = 10.81GGDD80 pKa = 3.59YY81 pKa = 11.08SPDD84 pKa = 3.26GDD86 pKa = 5.73DD87 pKa = 5.78YY88 pKa = 11.69IDD90 pKa = 5.71DD91 pKa = 5.48DD92 pKa = 6.52DD93 pKa = 6.51YY94 pKa = 12.07DD95 pKa = 5.62DD96 pKa = 6.0DD97 pKa = 3.85GWYY100 pKa = 10.29DD101 pKa = 3.65SDD103 pKa = 4.37SDD105 pKa = 4.65DD106 pKa = 3.71YY107 pKa = 11.89SSSEE111 pKa = 4.54DD112 pKa = 4.13CDD114 pKa = 5.11DD115 pKa = 6.23DD116 pKa = 6.1EE117 pKa = 8.23DD118 pKa = 6.68DD119 pKa = 6.38GDD121 pKa = 6.14DD122 pKa = 5.2DD123 pKa = 6.78DD124 pKa = 6.51DD125 pKa = 4.07DD126 pKa = 4.69HH127 pKa = 9.53RR128 pKa = 11.84EE129 pKa = 3.87EE130 pKa = 4.96PEE132 pKa = 3.81EE133 pKa = 3.98EE134 pKa = 4.42EE135 pKa = 4.34YY136 pKa = 11.15DD137 pKa = 3.74VLVAGGCSRR146 pKa = 11.84CYY148 pKa = 10.1MYY150 pKa = 10.96FLVPKK155 pKa = 9.86KK156 pKa = 11.03SNGCPRR162 pKa = 11.84CNGPLIRR169 pKa = 11.84FDD171 pKa = 3.68NGEE174 pKa = 3.9PEE176 pKa = 4.25YY177 pKa = 10.54PP178 pKa = 3.58

Molecular weight: 20.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A2ZPC3|A0A6A2ZPC3_HIBSY DnaJ protein ERDJ3B OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00110794pilonHSYRG00131 PE=4 SV=1
MM1 pKa = 7.14VVTHH5 pKa = 6.09HH6 pKa = 6.58RR7 pKa = 11.84ARR9 pKa = 11.84RR10 pKa = 11.84GVMTRR15 pKa = 11.84RR16 pKa = 11.84FWRR19 pKa = 11.84LRR21 pKa = 11.84WGGVALTAAWQQLKK35 pKa = 9.66RR36 pKa = 11.84QRR38 pKa = 11.84GGGGG42 pKa = 2.97

Molecular weight: 4.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82460

0

82460

32943284

30

8999

399.5

44.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.598 ± 0.008

1.912 ± 0.004

5.321 ± 0.006

6.405 ± 0.008

4.242 ± 0.005

6.546 ± 0.007

2.396 ± 0.004

5.266 ± 0.006

6.181 ± 0.008

9.522 ± 0.01

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.003

4.556 ± 0.005

4.918 ± 0.008

3.651 ± 0.006

5.398 ± 0.006

8.99 ± 0.01

4.937 ± 0.005

6.557 ± 0.006

1.346 ± 0.003

2.767 ± 0.004
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski