Microbacterium phage Musetta

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345L4S5|A0A345L4S5_9CAUD Uncharacterized protein OS=Microbacterium phage Musetta OX=2250324 GN=10 PE=4 SV=1
MM1 pKa = 7.66AIEE4 pKa = 4.3LQSLYY9 pKa = 11.03ACQTCYY15 pKa = 10.92LIVAGFDD22 pKa = 3.31PHH24 pKa = 7.12EE25 pKa = 5.02LGLPLSEE32 pKa = 5.08CAPTFDD38 pKa = 4.84GLSEE42 pKa = 4.35LEE44 pKa = 4.35GNVVTGDD51 pKa = 3.71PDD53 pKa = 3.36EE54 pKa = 4.58VRR56 pKa = 11.84PFDD59 pKa = 3.63TAPCDD64 pKa = 3.6VCGSGLAGEE73 pKa = 4.33RR74 pKa = 11.84SEE76 pKa = 4.65VVAITGEE83 pKa = 3.94RR84 pKa = 11.84DD85 pKa = 3.54TPGMHH90 pKa = 7.42ADD92 pKa = 3.26

Molecular weight: 9.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345L4M3|A0A345L4M3_9CAUD Uncharacterized protein OS=Microbacterium phage Musetta OX=2250324 GN=69 PE=4 SV=1
MM1 pKa = 7.14QRR3 pKa = 11.84TTRR6 pKa = 11.84FSEE9 pKa = 4.03GDD11 pKa = 2.88IVRR14 pKa = 11.84YY15 pKa = 9.42FEE17 pKa = 5.49GRR19 pKa = 11.84VDD21 pKa = 3.14WRR23 pKa = 11.84IKK25 pKa = 10.53HH26 pKa = 5.38VVTMSPRR33 pKa = 11.84KK34 pKa = 10.08DD35 pKa = 3.3GTVPFYY41 pKa = 11.35YY42 pKa = 9.93IMEE45 pKa = 4.52SGMSGRR51 pKa = 11.84RR52 pKa = 11.84VTAWPNEE59 pKa = 3.66VRR61 pKa = 11.84RR62 pKa = 11.84WVPGEE67 pKa = 3.81RR68 pKa = 3.64

Molecular weight: 8.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

18383

26

1283

168.7

18.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.548 ± 0.479

0.479 ± 0.09

6.479 ± 0.219

7.273 ± 0.343

3.302 ± 0.155

8.41 ± 0.278

1.817 ± 0.173

5.195 ± 0.165

4.591 ± 0.259

7.208 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.345 ± 0.202

3.802 ± 0.207

4.439 ± 0.252

3.182 ± 0.209

6.55 ± 0.201

5.886 ± 0.277

6.18 ± 0.284

7.338 ± 0.216

1.817 ± 0.135

3.161 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski