Absiella dolichum DSM 3991

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Amedibacillus; Amedibacillus dolichus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8R8I7|A8R8I7_9FIRM Uncharacterized protein OS=Absiella dolichum DSM 3991 OX=428127 GN=EUBDOL_00317 PE=4 SV=1
MM1 pKa = 7.85DD2 pKa = 4.55FEE4 pKa = 4.36TRR6 pKa = 11.84EE7 pKa = 4.07IVDD10 pKa = 3.2ILPNRR15 pKa = 11.84WEE17 pKa = 4.34TTLDD21 pKa = 3.1AYY23 pKa = 10.57FYY25 pKa = 10.58SISFQEE31 pKa = 4.2RR32 pKa = 11.84GNVEE36 pKa = 4.38YY37 pKa = 10.02IVSDD41 pKa = 3.81MYY43 pKa = 11.73SDD45 pKa = 3.45ILL47 pKa = 4.05

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8R8D4|A8R8D4_9FIRM SIS domain protein OS=Absiella dolichum DSM 3991 OX=428127 GN=EUBDOL_00268 PE=4 SV=1
MM1 pKa = 6.9MSKK4 pKa = 8.96TKK6 pKa = 10.61RR7 pKa = 11.84MTTITMLIALSIIFHH22 pKa = 5.91MVEE25 pKa = 3.89SMIPVPLPVPGFRR38 pKa = 11.84LGLANIVGLVAFYY51 pKa = 10.89LFNSRR56 pKa = 11.84VFIGVNFMRR65 pKa = 11.84VLLASLLRR73 pKa = 11.84GTLFGTGFWLSLCGVSFSCLACIVARR99 pKa = 11.84KK100 pKa = 5.54QTSMTIYY107 pKa = 8.78GVSVIGSVFHH117 pKa = 7.49CIGQVVAVTFIYY129 pKa = 10.4EE130 pKa = 4.08QFFMQAILPLLTLLAIPTGLFVALVAKK157 pKa = 9.55QVISRR162 pKa = 11.84IKK164 pKa = 9.24TRR166 pKa = 3.42

Molecular weight: 18.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2311

0

2311

648659

29

2685

280.7

31.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.127 ± 0.049

1.519 ± 0.023

5.53 ± 0.048

7.335 ± 0.059

4.3 ± 0.045

6.091 ± 0.052

2.154 ± 0.031

7.867 ± 0.055

7.536 ± 0.055

9.621 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.22 ± 0.031

4.583 ± 0.051

2.881 ± 0.028

3.745 ± 0.035

4.041 ± 0.039

5.757 ± 0.037

4.914 ± 0.041

6.602 ± 0.043

0.8 ± 0.016

4.375 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski