Amino acid dipepetide frequency for Gemmiger sp. An194

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.127AlaAla: 14.127 ± 0.198
1.665AlaCys: 1.665 ± 0.049
5.41AlaAsp: 5.41 ± 0.106
6.513AlaGlu: 6.513 ± 0.115
3.827AlaPhe: 3.827 ± 0.068
8.722AlaGly: 8.722 ± 0.123
1.601AlaHis: 1.601 ± 0.05
4.956AlaIle: 4.956 ± 0.088
4.535AlaLys: 4.535 ± 0.072
11.191AlaLeu: 11.191 ± 0.137
2.918AlaMet: 2.918 ± 0.057
2.783AlaAsn: 2.783 ± 0.059
3.977AlaPro: 3.977 ± 0.085
4.532AlaGln: 4.532 ± 0.092
5.284AlaArg: 5.284 ± 0.095
4.984AlaSer: 4.984 ± 0.084
4.079AlaThr: 4.079 ± 0.078
8.337AlaVal: 8.337 ± 0.119
1.055AlaTrp: 1.055 ± 0.034
2.789AlaTyr: 2.789 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
1.736CysAla: 1.736 ± 0.052
0.389CysCys: 0.389 ± 0.023
0.838CysAsp: 0.838 ± 0.033
0.997CysGlu: 0.997 ± 0.031
0.736CysPhe: 0.736 ± 0.029
1.871CysGly: 1.871 ± 0.052
0.338CysHis: 0.338 ± 0.021
0.846CysIle: 0.846 ± 0.033
0.655CysLys: 0.655 ± 0.029
1.546CysLeu: 1.546 ± 0.043
0.428CysMet: 0.428 ± 0.022
0.484CysAsn: 0.484 ± 0.025
0.88CysPro: 0.88 ± 0.037
0.538CysGln: 0.538 ± 0.026
1.035CysArg: 1.035 ± 0.038
0.918CysSer: 0.918 ± 0.035
0.98CysThr: 0.98 ± 0.03
1.096CysVal: 1.096 ± 0.04
0.185CysTrp: 0.185 ± 0.015
0.55CysTyr: 0.55 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
4.894AspAla: 4.894 ± 0.08
0.781AspCys: 0.781 ± 0.03
2.193AspAsp: 2.193 ± 0.056
4.021AspGlu: 4.021 ± 0.072
2.19AspPhe: 2.19 ± 0.049
4.359AspGly: 4.359 ± 0.093
0.887AspHis: 0.887 ± 0.032
2.645AspIle: 2.645 ± 0.057
2.397AspLys: 2.397 ± 0.063
4.888AspLeu: 4.888 ± 0.084
1.438AspMet: 1.438 ± 0.039
1.435AspAsn: 1.435 ± 0.044
2.561AspPro: 2.561 ± 0.055
1.834AspGln: 1.834 ± 0.042
2.804AspArg: 2.804 ± 0.061
2.601AspSer: 2.601 ± 0.054
2.844AspThr: 2.844 ± 0.057
3.627AspVal: 3.627 ± 0.071
0.833AspTrp: 0.833 ± 0.028
2.247AspTyr: 2.247 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
6.119GluAla: 6.119 ± 0.099
0.828GluCys: 0.828 ± 0.036
3.379GluAsp: 3.379 ± 0.064
5.574GluGlu: 5.574 ± 0.095
2.225GluPhe: 2.225 ± 0.049
4.793GluGly: 4.793 ± 0.081
1.279GluHis: 1.279 ± 0.042
3.315GluIle: 3.315 ± 0.067
4.194GluLys: 4.194 ± 0.079
6.88GluLeu: 6.88 ± 0.107
2.019GluMet: 2.019 ± 0.051
3.185GluAsn: 3.185 ± 0.065
2.49GluPro: 2.49 ± 0.063
3.744GluGln: 3.744 ± 0.081
3.5GluArg: 3.5 ± 0.069
3.128GluSer: 3.128 ± 0.056
3.398GluThr: 3.398 ± 0.072
3.687GluVal: 3.687 ± 0.075
0.692GluTrp: 0.692 ± 0.028
2.526GluTyr: 2.526 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
4.091PheAla: 4.091 ± 0.073
0.906PheCys: 0.906 ± 0.032
2.367PheAsp: 2.367 ± 0.054
2.296PheGlu: 2.296 ± 0.049
1.733PhePhe: 1.733 ± 0.049
3.394PheGly: 3.394 ± 0.068
0.683PheHis: 0.683 ± 0.03
1.745PheIle: 1.745 ± 0.047
1.282PheLys: 1.282 ± 0.041
3.78PheLeu: 3.78 ± 0.081
0.842PheMet: 0.842 ± 0.035
1.22PheAsn: 1.22 ± 0.032
1.299PhePro: 1.299 ± 0.037
1.081PheGln: 1.081 ± 0.037
1.702PheArg: 1.702 ± 0.049
2.728PheSer: 2.728 ± 0.061
2.405PheThr: 2.405 ± 0.055
2.983PheVal: 2.983 ± 0.067
0.579PheTrp: 0.579 ± 0.03
1.515PheTyr: 1.515 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
7.045GlyAla: 7.045 ± 0.103
1.658GlyCys: 1.658 ± 0.05
3.721GlyAsp: 3.721 ± 0.068
5.053GlyGlu: 5.053 ± 0.08
3.315GlyPhe: 3.315 ± 0.066
6.253GlyGly: 6.253 ± 0.098
1.363GlyHis: 1.363 ± 0.043
4.529GlyIle: 4.529 ± 0.074
4.16GlyLys: 4.16 ± 0.064
7.968GlyLeu: 7.968 ± 0.115
2.596GlyMet: 2.596 ± 0.055
2.389GlyAsn: 2.389 ± 0.058
2.306GlyPro: 2.306 ± 0.064
3.298GlyGln: 3.298 ± 0.068
4.485GlyArg: 4.485 ± 0.079
4.522GlySer: 4.522 ± 0.086
4.214GlyThr: 4.214 ± 0.083
6.378GlyVal: 6.378 ± 0.094
1.111GlyTrp: 1.111 ± 0.037
2.98GlyTyr: 2.98 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.334HisAla: 1.334 ± 0.038
0.366HisCys: 0.366 ± 0.024
0.781HisAsp: 0.781 ± 0.035
0.87HisGlu: 0.87 ± 0.034
0.778HisPhe: 0.778 ± 0.026
1.303HisGly: 1.303 ± 0.04
0.417HisHis: 0.417 ± 0.027
1.079HisIle: 1.079 ± 0.041
0.772HisLys: 0.772 ± 0.029
1.716HisLeu: 1.716 ± 0.046
0.422HisMet: 0.422 ± 0.023
0.615HisAsn: 0.615 ± 0.028
1.075HisPro: 1.075 ± 0.038
0.576HisGln: 0.576 ± 0.027
1.03HisArg: 1.03 ± 0.032
1.042HisSer: 1.042 ± 0.032
1.046HisThr: 1.046 ± 0.033
0.973HisVal: 0.973 ± 0.033
0.263HisTrp: 0.263 ± 0.017
0.71HisTyr: 0.71 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
5.117IleAla: 5.117 ± 0.083
0.978IleCys: 0.978 ± 0.035
2.759IleAsp: 2.759 ± 0.063
2.973IleGlu: 2.973 ± 0.07
1.81IlePhe: 1.81 ± 0.05
3.895IleGly: 3.895 ± 0.078
0.988IleHis: 0.988 ± 0.032
2.468IleIle: 2.468 ± 0.068
2.005IleLys: 2.005 ± 0.056
5.508IleLeu: 5.508 ± 0.082
1.128IleMet: 1.128 ± 0.035
1.673IleAsn: 1.673 ± 0.041
2.707IlePro: 2.707 ± 0.065
2.065IleGln: 2.065 ± 0.055
3.094IleArg: 3.094 ± 0.06
3.088IleSer: 3.088 ± 0.065
3.249IleThr: 3.249 ± 0.063
3.588IleVal: 3.588 ± 0.078
0.59IleTrp: 0.59 ± 0.024
1.677IleTyr: 1.677 ± 0.043
0.0IleXaa: 0.0 ± 0.0
Lys
4.976LysAla: 4.976 ± 0.083
0.447LysCys: 0.447 ± 0.028
2.241LysAsp: 2.241 ± 0.057
3.457LysGlu: 3.457 ± 0.08
1.203LysPhe: 1.203 ± 0.04
3.364LysGly: 3.364 ± 0.076
0.714LysHis: 0.714 ± 0.026
2.302LysIle: 2.302 ± 0.051
3.233LysLys: 3.233 ± 0.083
4.311LysLeu: 4.311 ± 0.071
1.3LysMet: 1.3 ± 0.038
2.117LysAsn: 2.117 ± 0.048
1.927LysPro: 1.927 ± 0.052
2.034LysGln: 2.034 ± 0.051
2.473LysArg: 2.473 ± 0.065
2.221LysSer: 2.221 ± 0.047
2.719LysThr: 2.719 ± 0.063
2.828LysVal: 2.828 ± 0.068
0.443LysTrp: 0.443 ± 0.025
1.448LysTyr: 1.448 ± 0.044
0.0LysXaa: 0.0 ± 0.0
Leu
11.985LeuAla: 11.985 ± 0.159
2.256LeuCys: 2.256 ± 0.062
5.486LeuAsp: 5.486 ± 0.083
7.004LeuGlu: 7.004 ± 0.097
4.103LeuPhe: 4.103 ± 0.09
7.342LeuGly: 7.342 ± 0.111
1.796LeuHis: 1.796 ± 0.048
4.705LeuIle: 4.705 ± 0.089
4.041LeuLys: 4.041 ± 0.077
10.872LeuLeu: 10.872 ± 0.192
2.617LeuMet: 2.617 ± 0.05
3.393LeuAsn: 3.393 ± 0.062
5.175LeuPro: 5.175 ± 0.09
3.484LeuGln: 3.484 ± 0.068
5.744LeuArg: 5.744 ± 0.097
5.941LeuSer: 5.941 ± 0.086
5.776LeuThr: 5.776 ± 0.093
7.165LeuVal: 7.165 ± 0.111
1.209LeuTrp: 1.209 ± 0.042
3.263LeuTyr: 3.263 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
2.892MetAla: 2.892 ± 0.065
0.334MetCys: 0.334 ± 0.022
1.663MetAsp: 1.663 ± 0.039
2.136MetGlu: 2.136 ± 0.053
0.867MetPhe: 0.867 ± 0.031
2.128MetGly: 2.128 ± 0.049
0.398MetHis: 0.398 ± 0.02
1.397MetIle: 1.397 ± 0.035
1.639MetLys: 1.639 ± 0.041
2.76MetLeu: 2.76 ± 0.062
0.768MetMet: 0.768 ± 0.033
1.208MetAsn: 1.208 ± 0.036
1.326MetPro: 1.326 ± 0.04
1.096MetGln: 1.096 ± 0.037
1.323MetArg: 1.323 ± 0.036
1.464MetSer: 1.464 ± 0.043
1.502MetThr: 1.502 ± 0.04
1.858MetVal: 1.858 ± 0.046
0.213MetTrp: 0.213 ± 0.015
0.736MetTyr: 0.736 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.168AsnAla: 3.168 ± 0.063
0.546AsnCys: 0.546 ± 0.023
1.58AsnAsp: 1.58 ± 0.047
2.027AsnGlu: 2.027 ± 0.048
1.248AsnPhe: 1.248 ± 0.039
3.22AsnGly: 3.22 ± 0.074
0.585AsnHis: 0.585 ± 0.027
1.957AsnIle: 1.957 ± 0.048
1.429AsnLys: 1.429 ± 0.038
3.225AsnLeu: 3.225 ± 0.064
0.932AsnMet: 0.932 ± 0.035
1.164AsnAsn: 1.164 ± 0.039
1.825AsnPro: 1.825 ± 0.054
1.25AsnGln: 1.25 ± 0.038
1.772AsnArg: 1.772 ± 0.052
1.694AsnSer: 1.694 ± 0.05
1.985AsnThr: 1.985 ± 0.054
2.249AsnVal: 2.249 ± 0.06
0.432AsnTrp: 0.432 ± 0.025
1.272AsnTyr: 1.272 ± 0.039
0.0AsnXaa: 0.0 ± 0.0
Pro
5.025ProAla: 5.025 ± 0.095
0.652ProCys: 0.652 ± 0.026
2.789ProAsp: 2.789 ± 0.056
4.05ProGlu: 4.05 ± 0.067
1.699ProPhe: 1.699 ± 0.044
3.686ProGly: 3.686 ± 0.062
0.694ProHis: 0.694 ± 0.028
1.903ProIle: 1.903 ± 0.055
1.589ProLys: 1.589 ± 0.04
3.992ProLeu: 3.992 ± 0.074
1.142ProMet: 1.142 ± 0.037
1.256ProAsn: 1.256 ± 0.036
1.339ProPro: 1.339 ± 0.044
1.818ProGln: 1.818 ± 0.046
1.794ProArg: 1.794 ± 0.041
1.949ProSer: 1.949 ± 0.049
2.14ProThr: 2.14 ± 0.072
3.867ProVal: 3.867 ± 0.067
0.485ProTrp: 0.485 ± 0.025
1.387ProTyr: 1.387 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
4.384GlnAla: 4.384 ± 0.075
0.455GlnCys: 0.455 ± 0.02
1.814GlnAsp: 1.814 ± 0.042
2.525GlnGlu: 2.525 ± 0.062
1.244GlnPhe: 1.244 ± 0.036
2.904GlnGly: 2.904 ± 0.063
0.598GlnHis: 0.598 ± 0.026
2.165GlnIle: 2.165 ± 0.051
2.304GlnLys: 2.304 ± 0.06
4.632GlnLeu: 4.632 ± 0.087
1.294GlnMet: 1.294 ± 0.038
1.579GlnAsn: 1.579 ± 0.051
1.974GlnPro: 1.974 ± 0.051
2.171GlnGln: 2.171 ± 0.066
2.244GlnArg: 2.244 ± 0.058
2.049GlnSer: 2.049 ± 0.051
2.092GlnThr: 2.092 ± 0.049
2.926GlnVal: 2.926 ± 0.056
0.512GlnTrp: 0.512 ± 0.025
1.281GlnTyr: 1.281 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
4.817ArgAla: 4.817 ± 0.088
0.992ArgCys: 0.992 ± 0.038
2.467ArgAsp: 2.467 ± 0.059
3.419ArgGlu: 3.419 ± 0.072
2.36ArgPhe: 2.36 ± 0.059
3.163ArgGly: 3.163 ± 0.066
0.958ArgHis: 0.958 ± 0.037
3.21ArgIle: 3.21 ± 0.064
2.532ArgLys: 2.532 ± 0.063
5.827ArgLeu: 5.827 ± 0.1
1.75ArgMet: 1.75 ± 0.043
1.562ArgAsn: 1.562 ± 0.046
2.295ArgPro: 2.295 ± 0.052
2.937ArgGln: 2.937 ± 0.056
4.068ArgArg: 4.068 ± 0.096
2.647ArgSer: 2.647 ± 0.058
2.7ArgThr: 2.7 ± 0.056
3.602ArgVal: 3.602 ± 0.066
0.779ArgTrp: 0.779 ± 0.036
2.045ArgTyr: 2.045 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
5.386SerAla: 5.386 ± 0.084
0.82SerCys: 0.82 ± 0.027
2.585SerAsp: 2.585 ± 0.064
3.275SerGlu: 3.275 ± 0.076
2.329SerPhe: 2.329 ± 0.055
5.165SerGly: 5.165 ± 0.086
0.944SerHis: 0.944 ± 0.03
3.003SerIle: 3.003 ± 0.071
1.91SerLys: 1.91 ± 0.05
5.431SerLeu: 5.431 ± 0.097
1.594SerMet: 1.594 ± 0.046
1.518SerAsn: 1.518 ± 0.043
2.198SerPro: 2.198 ± 0.046
2.04SerGln: 2.04 ± 0.051
2.907SerArg: 2.907 ± 0.062
3.127SerSer: 3.127 ± 0.087
2.915SerThr: 2.915 ± 0.066
3.986SerVal: 3.986 ± 0.078
0.619SerTrp: 0.619 ± 0.029
1.891SerTyr: 1.891 ± 0.049
0.0SerXaa: 0.0 ± 0.0
Thr
5.835ThrAla: 5.835 ± 0.106
0.673ThrCys: 0.673 ± 0.028
2.856ThrAsp: 2.856 ± 0.078
3.248ThrGlu: 3.248 ± 0.074
1.889ThrPhe: 1.889 ± 0.048
5.404ThrGly: 5.404 ± 0.074
0.877ThrHis: 0.877 ± 0.031
2.98ThrIle: 2.98 ± 0.063
1.882ThrLys: 1.882 ± 0.046
5.752ThrLeu: 5.752 ± 0.084
1.334ThrMet: 1.334 ± 0.039
1.598ThrAsn: 1.598 ± 0.045
2.881ThrPro: 2.881 ± 0.065
1.801ThrGln: 1.801 ± 0.045
2.535ThrArg: 2.535 ± 0.053
2.69ThrSer: 2.69 ± 0.065
2.727ThrThr: 2.727 ± 0.069
4.767ThrVal: 4.767 ± 0.083
0.549ThrTrp: 0.549 ± 0.025
1.49ThrTyr: 1.49 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
6.275ValAla: 6.275 ± 0.109
1.505ValCys: 1.505 ± 0.046
3.994ValAsp: 3.994 ± 0.076
4.673ValGlu: 4.673 ± 0.082
3.08ValPhe: 3.08 ± 0.073
4.746ValGly: 4.746 ± 0.081
1.11ValHis: 1.11 ± 0.04
4.021ValIle: 4.021 ± 0.077
3.224ValLys: 3.224 ± 0.07
8.075ValLeu: 8.075 ± 0.125
1.989ValMet: 1.989 ± 0.054
2.547ValAsn: 2.547 ± 0.059
3.122ValPro: 3.122 ± 0.068
2.615ValGln: 2.615 ± 0.068
3.687ValArg: 3.687 ± 0.074
4.305ValSer: 4.305 ± 0.066
4.323ValThr: 4.323 ± 0.094
5.344ValVal: 5.344 ± 0.098
0.899ValTrp: 0.899 ± 0.032
2.574ValTyr: 2.574 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
1.081TrpAla: 1.081 ± 0.04
0.215TrpCys: 0.215 ± 0.016
0.57TrpAsp: 0.57 ± 0.027
0.716TrpGlu: 0.716 ± 0.031
0.496TrpPhe: 0.496 ± 0.026
0.938TrpGly: 0.938 ± 0.039
0.252TrpHis: 0.252 ± 0.019
0.508TrpIle: 0.508 ± 0.025
0.54TrpLys: 0.54 ± 0.022
1.458TrpLeu: 1.458 ± 0.042
0.378TrpMet: 0.378 ± 0.021
0.515TrpAsn: 0.515 ± 0.024
0.475TrpPro: 0.475 ± 0.023
0.736TrpGln: 0.736 ± 0.029
0.623TrpArg: 0.623 ± 0.029
0.557TrpSer: 0.557 ± 0.025
0.531TrpThr: 0.531 ± 0.025
0.808TrpVal: 0.808 ± 0.033
0.165TrpTrp: 0.165 ± 0.016
0.466TrpTyr: 0.466 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.994TyrAla: 2.994 ± 0.056
0.582TyrCys: 0.582 ± 0.025
1.986TyrAsp: 1.986 ± 0.049
2.125TyrGlu: 2.125 ± 0.046
1.501TyrPhe: 1.501 ± 0.043
2.694TyrGly: 2.694 ± 0.052
0.685TyrHis: 0.685 ± 0.028
1.628TyrIle: 1.628 ± 0.042
1.435TyrLys: 1.435 ± 0.044
3.545TyrLeu: 3.545 ± 0.069
0.814TyrMet: 0.814 ± 0.027
1.315TyrAsn: 1.315 ± 0.048
1.424TyrPro: 1.424 ± 0.037
1.497TyrGln: 1.497 ± 0.045
2.029TyrArg: 2.029 ± 0.044
1.993TyrSer: 1.993 ± 0.046
2.143TyrThr: 2.143 ± 0.05
2.04TyrVal: 2.04 ± 0.048
0.415TyrTrp: 0.415 ± 0.024
1.431TyrTyr: 1.431 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2772 proteins (896356 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski