Amino acid dipepetide frequency for Paenibacillus phage Lucielle

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.948AlaAla: 4.948 ± 1.012
0.512AlaCys: 0.512 ± 0.171
4.862AlaAsp: 4.862 ± 0.732
6.142AlaGlu: 6.142 ± 0.725
1.706AlaPhe: 1.706 ± 0.402
4.436AlaGly: 4.436 ± 0.662
0.853AlaHis: 0.853 ± 0.274
4.692AlaIle: 4.692 ± 0.812
5.289AlaLys: 5.289 ± 0.706
5.545AlaLeu: 5.545 ± 0.734
1.28AlaMet: 1.28 ± 0.324
3.156AlaAsn: 3.156 ± 0.454
1.706AlaPro: 1.706 ± 0.383
2.133AlaGln: 2.133 ± 0.377
1.877AlaArg: 1.877 ± 0.374
4.009AlaSer: 4.009 ± 0.641
3.839AlaThr: 3.839 ± 0.829
4.35AlaVal: 4.35 ± 0.534
1.365AlaTrp: 1.365 ± 0.409
2.388AlaTyr: 2.388 ± 0.426
0.0AlaXaa: 0.0 ± 0.0
Cys
0.341CysAla: 0.341 ± 0.157
0.341CysCys: 0.341 ± 0.228
0.597CysAsp: 0.597 ± 0.219
0.512CysGlu: 0.512 ± 0.253
0.341CysPhe: 0.341 ± 0.268
0.597CysGly: 0.597 ± 0.245
0.171CysHis: 0.171 ± 0.125
0.427CysIle: 0.427 ± 0.22
1.28CysLys: 1.28 ± 0.372
1.109CysLeu: 1.109 ± 0.283
0.341CysMet: 0.341 ± 0.161
0.256CysAsn: 0.256 ± 0.155
0.341CysPro: 0.341 ± 0.233
0.341CysGln: 0.341 ± 0.181
0.341CysArg: 0.341 ± 0.168
0.597CysSer: 0.597 ± 0.203
0.341CysThr: 0.341 ± 0.147
0.512CysVal: 0.512 ± 0.194
0.256CysTrp: 0.256 ± 0.208
0.341CysTyr: 0.341 ± 0.184
0.0CysXaa: 0.0 ± 0.0
Asp
4.095AspAla: 4.095 ± 0.671
0.341AspCys: 0.341 ± 0.174
4.18AspAsp: 4.18 ± 0.698
4.009AspGlu: 4.009 ± 0.697
3.839AspPhe: 3.839 ± 0.588
4.35AspGly: 4.35 ± 0.506
0.768AspHis: 0.768 ± 0.256
3.497AspIle: 3.497 ± 0.455
3.924AspLys: 3.924 ± 0.583
5.118AspLeu: 5.118 ± 0.596
2.303AspMet: 2.303 ± 0.412
2.047AspAsn: 2.047 ± 0.357
2.218AspPro: 2.218 ± 0.41
2.218AspGln: 2.218 ± 0.459
2.474AspArg: 2.474 ± 0.514
2.9AspSer: 2.9 ± 0.496
3.241AspThr: 3.241 ± 0.45
4.521AspVal: 4.521 ± 0.662
0.853AspTrp: 0.853 ± 0.308
2.474AspTyr: 2.474 ± 0.469
0.0AspXaa: 0.0 ± 0.0
Glu
5.801GluAla: 5.801 ± 0.859
0.427GluCys: 0.427 ± 0.197
5.715GluAsp: 5.715 ± 0.748
6.398GluGlu: 6.398 ± 0.885
2.815GluPhe: 2.815 ± 0.39
3.583GluGly: 3.583 ± 0.463
1.194GluHis: 1.194 ± 0.308
6.398GluIle: 6.398 ± 0.819
7.763GluLys: 7.763 ± 0.865
7.251GluLeu: 7.251 ± 0.829
4.521GluMet: 4.521 ± 0.639
3.924GluAsn: 3.924 ± 0.684
2.474GluPro: 2.474 ± 0.411
3.839GluGln: 3.839 ± 0.779
4.692GluArg: 4.692 ± 0.872
3.839GluSer: 3.839 ± 0.528
4.777GluThr: 4.777 ± 0.549
5.63GluVal: 5.63 ± 0.802
1.109GluTrp: 1.109 ± 0.313
3.412GluTyr: 3.412 ± 0.507
0.0GluXaa: 0.0 ± 0.0
Phe
1.791PheAla: 1.791 ± 0.349
0.768PheCys: 0.768 ± 0.226
2.73PheAsp: 2.73 ± 0.453
3.583PheGlu: 3.583 ± 0.561
0.938PhePhe: 0.938 ± 0.312
2.133PheGly: 2.133 ± 0.437
1.194PheHis: 1.194 ± 0.379
3.071PheIle: 3.071 ± 0.485
2.559PheLys: 2.559 ± 0.534
2.218PheLeu: 2.218 ± 0.386
1.109PheMet: 1.109 ± 0.364
2.133PheAsn: 2.133 ± 0.393
0.682PhePro: 0.682 ± 0.241
1.621PheGln: 1.621 ± 0.358
1.194PheArg: 1.194 ± 0.32
2.73PheSer: 2.73 ± 0.448
1.45PheThr: 1.45 ± 0.331
2.986PheVal: 2.986 ± 0.502
0.427PheTrp: 0.427 ± 0.171
1.45PheTyr: 1.45 ± 0.347
0.0PheXaa: 0.0 ± 0.0
Gly
3.412GlyAla: 3.412 ± 0.499
1.024GlyCys: 1.024 ± 0.415
2.986GlyAsp: 2.986 ± 0.448
5.971GlyGlu: 5.971 ± 0.767
2.218GlyPhe: 2.218 ± 0.447
2.986GlyGly: 2.986 ± 0.508
0.938GlyHis: 0.938 ± 0.279
5.886GlyIle: 5.886 ± 1.099
6.909GlyLys: 6.909 ± 0.721
4.948GlyLeu: 4.948 ± 0.878
1.962GlyMet: 1.962 ± 0.649
3.071GlyAsn: 3.071 ± 0.59
0.938GlyPro: 0.938 ± 0.352
2.303GlyGln: 2.303 ± 0.452
3.071GlyArg: 3.071 ± 0.54
3.241GlySer: 3.241 ± 0.65
2.474GlyThr: 2.474 ± 0.615
4.009GlyVal: 4.009 ± 0.526
1.024GlyTrp: 1.024 ± 0.238
2.218GlyTyr: 2.218 ± 0.417
0.0GlyXaa: 0.0 ± 0.0
His
1.28HisAla: 1.28 ± 0.281
0.341HisCys: 0.341 ± 0.164
0.512HisAsp: 0.512 ± 0.234
1.365HisGlu: 1.365 ± 0.362
0.682HisPhe: 0.682 ± 0.203
0.938HisGly: 0.938 ± 0.295
0.512HisHis: 0.512 ± 0.176
2.133HisIle: 2.133 ± 0.551
0.938HisLys: 0.938 ± 0.308
1.877HisLeu: 1.877 ± 0.421
0.341HisMet: 0.341 ± 0.146
1.194HisAsn: 1.194 ± 0.302
0.938HisPro: 0.938 ± 0.264
0.597HisGln: 0.597 ± 0.189
1.194HisArg: 1.194 ± 0.268
1.024HisSer: 1.024 ± 0.277
0.597HisThr: 0.597 ± 0.254
1.109HisVal: 1.109 ± 0.309
0.171HisTrp: 0.171 ± 0.116
0.341HisTyr: 0.341 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
4.18IleAla: 4.18 ± 0.785
0.682IleCys: 0.682 ± 0.288
4.606IleAsp: 4.606 ± 0.782
6.824IleGlu: 6.824 ± 0.814
3.071IlePhe: 3.071 ± 0.514
3.668IleGly: 3.668 ± 0.668
1.706IleHis: 1.706 ± 0.333
2.986IleIle: 2.986 ± 0.692
7.251IleLys: 7.251 ± 1.074
5.63IleLeu: 5.63 ± 0.687
1.365IleMet: 1.365 ± 0.365
3.839IleAsn: 3.839 ± 0.635
2.644IlePro: 2.644 ± 0.469
2.815IleGln: 2.815 ± 0.474
3.753IleArg: 3.753 ± 0.681
3.241IleSer: 3.241 ± 0.554
4.436IleThr: 4.436 ± 0.535
4.521IleVal: 4.521 ± 0.663
0.682IleTrp: 0.682 ± 0.252
2.815IleTyr: 2.815 ± 0.508
0.0IleXaa: 0.0 ± 0.0
Lys
6.056LysAla: 6.056 ± 0.65
0.341LysCys: 0.341 ± 0.186
4.265LysAsp: 4.265 ± 0.5
9.213LysGlu: 9.213 ± 0.981
2.133LysPhe: 2.133 ± 0.363
5.801LysGly: 5.801 ± 0.628
1.706LysHis: 1.706 ± 0.327
6.227LysIle: 6.227 ± 0.677
7.933LysLys: 7.933 ± 1.132
6.654LysLeu: 6.654 ± 0.808
3.071LysMet: 3.071 ± 0.453
3.753LysAsn: 3.753 ± 0.667
2.559LysPro: 2.559 ± 0.545
4.095LysGln: 4.095 ± 0.696
5.033LysArg: 5.033 ± 0.614
5.289LysSer: 5.289 ± 0.722
5.801LysThr: 5.801 ± 0.773
4.606LysVal: 4.606 ± 0.587
1.28LysTrp: 1.28 ± 0.346
3.241LysTyr: 3.241 ± 0.582
0.0LysXaa: 0.0 ± 0.0
Leu
6.312LeuAla: 6.312 ± 0.776
0.682LeuCys: 0.682 ± 0.331
4.777LeuAsp: 4.777 ± 0.674
7.763LeuGlu: 7.763 ± 0.918
2.388LeuPhe: 2.388 ± 0.482
4.606LeuGly: 4.606 ± 0.492
1.791LeuHis: 1.791 ± 0.362
4.777LeuIle: 4.777 ± 0.526
6.995LeuLys: 6.995 ± 0.7
4.692LeuLeu: 4.692 ± 0.677
1.621LeuMet: 1.621 ± 0.425
4.095LeuAsn: 4.095 ± 0.451
3.327LeuPro: 3.327 ± 0.506
4.18LeuGln: 4.18 ± 0.574
3.327LeuArg: 3.327 ± 0.484
5.374LeuSer: 5.374 ± 0.549
5.289LeuThr: 5.289 ± 0.711
3.753LeuVal: 3.753 ± 0.567
0.853LeuTrp: 0.853 ± 0.364
2.986LeuTyr: 2.986 ± 0.47
0.0LeuXaa: 0.0 ± 0.0
Met
2.047MetAla: 2.047 ± 0.367
0.0MetCys: 0.0 ± 0.0
1.706MetAsp: 1.706 ± 0.371
2.559MetGlu: 2.559 ± 0.407
1.109MetPhe: 1.109 ± 0.233
1.194MetGly: 1.194 ± 0.313
0.085MetHis: 0.085 ± 0.093
2.218MetIle: 2.218 ± 0.462
3.412MetLys: 3.412 ± 0.605
2.218MetLeu: 2.218 ± 0.513
0.512MetMet: 0.512 ± 0.22
2.474MetAsn: 2.474 ± 0.378
1.109MetPro: 1.109 ± 0.298
1.535MetGln: 1.535 ± 0.315
1.45MetArg: 1.45 ± 0.315
2.388MetSer: 2.388 ± 0.402
1.365MetThr: 1.365 ± 0.313
0.938MetVal: 0.938 ± 0.299
0.341MetTrp: 0.341 ± 0.145
1.024MetTyr: 1.024 ± 0.297
0.0MetXaa: 0.0 ± 0.0
Asn
2.644AsnAla: 2.644 ± 0.492
0.512AsnCys: 0.512 ± 0.216
2.388AsnAsp: 2.388 ± 0.501
3.753AsnGlu: 3.753 ± 0.676
1.535AsnPhe: 1.535 ± 0.407
4.606AsnGly: 4.606 ± 0.644
0.853AsnHis: 0.853 ± 0.256
3.412AsnIle: 3.412 ± 0.513
3.583AsnLys: 3.583 ± 0.525
3.924AsnLeu: 3.924 ± 0.644
1.28AsnMet: 1.28 ± 0.397
1.706AsnAsn: 1.706 ± 0.516
2.559AsnPro: 2.559 ± 0.465
1.621AsnGln: 1.621 ± 0.401
3.327AsnArg: 3.327 ± 0.637
2.388AsnSer: 2.388 ± 0.411
2.559AsnThr: 2.559 ± 0.46
2.388AsnVal: 2.388 ± 0.481
0.427AsnTrp: 0.427 ± 0.224
1.365AsnTyr: 1.365 ± 0.293
0.0AsnXaa: 0.0 ± 0.0
Pro
2.133ProAla: 2.133 ± 0.395
0.085ProCys: 0.085 ± 0.079
1.791ProAsp: 1.791 ± 0.408
2.986ProGlu: 2.986 ± 0.766
1.45ProPhe: 1.45 ± 0.364
2.388ProGly: 2.388 ± 0.419
0.853ProHis: 0.853 ± 0.271
2.644ProIle: 2.644 ± 0.433
3.071ProLys: 3.071 ± 0.57
2.474ProLeu: 2.474 ± 0.419
0.853ProMet: 0.853 ± 0.289
1.28ProAsn: 1.28 ± 0.305
0.938ProPro: 0.938 ± 0.288
0.938ProGln: 0.938 ± 0.362
1.024ProArg: 1.024 ± 0.284
2.73ProSer: 2.73 ± 0.604
1.706ProThr: 1.706 ± 0.342
1.962ProVal: 1.962 ± 0.536
0.597ProTrp: 0.597 ± 0.189
1.28ProTyr: 1.28 ± 0.309
0.0ProXaa: 0.0 ± 0.0
Gln
3.839GlnAla: 3.839 ± 0.597
0.256GlnCys: 0.256 ± 0.139
2.047GlnAsp: 2.047 ± 0.363
3.753GlnGlu: 3.753 ± 0.593
1.791GlnPhe: 1.791 ± 0.356
2.474GlnGly: 2.474 ± 0.567
0.938GlnHis: 0.938 ± 0.243
2.388GlnIle: 2.388 ± 0.507
3.071GlnLys: 3.071 ± 0.574
3.241GlnLeu: 3.241 ± 0.689
1.706GlnMet: 1.706 ± 0.413
1.535GlnAsn: 1.535 ± 0.281
1.877GlnPro: 1.877 ± 0.541
1.877GlnGln: 1.877 ± 0.429
2.133GlnArg: 2.133 ± 0.439
2.303GlnSer: 2.303 ± 0.372
2.303GlnThr: 2.303 ± 0.415
1.962GlnVal: 1.962 ± 0.484
0.341GlnTrp: 0.341 ± 0.213
0.938GlnTyr: 0.938 ± 0.258
0.0GlnXaa: 0.0 ± 0.0
Arg
2.644ArgAla: 2.644 ± 0.455
0.512ArgCys: 0.512 ± 0.221
3.241ArgAsp: 3.241 ± 0.453
4.009ArgGlu: 4.009 ± 0.771
1.45ArgPhe: 1.45 ± 0.448
2.644ArgGly: 2.644 ± 0.497
0.768ArgHis: 0.768 ± 0.25
2.815ArgIle: 2.815 ± 0.448
5.459ArgLys: 5.459 ± 0.956
4.35ArgLeu: 4.35 ± 0.758
1.621ArgMet: 1.621 ± 0.434
2.303ArgAsn: 2.303 ± 0.446
1.621ArgPro: 1.621 ± 0.382
2.644ArgGln: 2.644 ± 0.406
2.388ArgArg: 2.388 ± 0.456
1.962ArgSer: 1.962 ± 0.345
1.706ArgThr: 1.706 ± 0.363
2.815ArgVal: 2.815 ± 0.544
0.682ArgTrp: 0.682 ± 0.253
1.962ArgTyr: 1.962 ± 0.393
0.0ArgXaa: 0.0 ± 0.0
Ser
5.118SerAla: 5.118 ± 0.96
0.341SerCys: 0.341 ± 0.156
3.497SerAsp: 3.497 ± 0.606
4.606SerGlu: 4.606 ± 0.611
2.474SerPhe: 2.474 ± 0.463
4.095SerGly: 4.095 ± 0.648
1.024SerHis: 1.024 ± 0.318
5.801SerIle: 5.801 ± 0.713
4.265SerLys: 4.265 ± 0.72
4.948SerLeu: 4.948 ± 0.852
1.535SerMet: 1.535 ± 0.352
1.962SerAsn: 1.962 ± 0.367
1.791SerPro: 1.791 ± 0.347
1.962SerGln: 1.962 ± 0.462
2.73SerArg: 2.73 ± 0.47
3.668SerSer: 3.668 ± 0.646
3.327SerThr: 3.327 ± 0.462
3.839SerVal: 3.839 ± 0.692
0.427SerTrp: 0.427 ± 0.188
1.791SerTyr: 1.791 ± 0.345
0.0SerXaa: 0.0 ± 0.0
Thr
3.497ThrAla: 3.497 ± 0.644
0.341ThrCys: 0.341 ± 0.191
2.218ThrAsp: 2.218 ± 0.404
3.753ThrGlu: 3.753 ± 0.485
1.621ThrPhe: 1.621 ± 0.394
4.521ThrGly: 4.521 ± 0.64
1.28ThrHis: 1.28 ± 0.348
4.18ThrIle: 4.18 ± 0.577
4.692ThrLys: 4.692 ± 0.665
4.692ThrLeu: 4.692 ± 0.701
1.365ThrMet: 1.365 ± 0.398
1.877ThrAsn: 1.877 ± 0.391
2.815ThrPro: 2.815 ± 0.53
2.474ThrGln: 2.474 ± 0.443
2.133ThrArg: 2.133 ± 0.4
3.497ThrSer: 3.497 ± 0.534
2.047ThrThr: 2.047 ± 0.47
4.436ThrVal: 4.436 ± 0.562
0.512ThrTrp: 0.512 ± 0.258
2.388ThrTyr: 2.388 ± 0.44
0.0ThrXaa: 0.0 ± 0.0
Val
3.071ValAla: 3.071 ± 0.559
0.853ValCys: 0.853 ± 0.31
3.412ValAsp: 3.412 ± 0.618
4.521ValGlu: 4.521 ± 0.743
2.815ValPhe: 2.815 ± 0.484
3.412ValGly: 3.412 ± 0.599
0.597ValHis: 0.597 ± 0.311
3.412ValIle: 3.412 ± 0.455
6.142ValLys: 6.142 ± 0.724
4.436ValLeu: 4.436 ± 0.572
1.791ValMet: 1.791 ± 0.362
3.156ValAsn: 3.156 ± 0.625
1.535ValPro: 1.535 ± 0.334
1.621ValGln: 1.621 ± 0.385
2.986ValArg: 2.986 ± 0.515
5.289ValSer: 5.289 ± 0.781
4.777ValThr: 4.777 ± 0.655
3.753ValVal: 3.753 ± 0.479
1.962ValTrp: 1.962 ± 0.967
2.388ValTyr: 2.388 ± 0.599
0.0ValXaa: 0.0 ± 0.0
Trp
0.512TrpAla: 0.512 ± 0.167
0.256TrpCys: 0.256 ± 0.16
0.768TrpAsp: 0.768 ± 0.336
1.024TrpGlu: 1.024 ± 0.228
0.938TrpPhe: 0.938 ± 0.258
1.024TrpGly: 1.024 ± 0.289
0.427TrpHis: 0.427 ± 0.176
0.938TrpIle: 0.938 ± 0.367
1.024TrpLys: 1.024 ± 0.274
1.535TrpLeu: 1.535 ± 0.337
0.171TrpMet: 0.171 ± 0.132
1.706TrpAsn: 1.706 ± 1.119
0.0TrpPro: 0.0 ± 0.0
0.682TrpGln: 0.682 ± 0.232
0.341TrpArg: 0.341 ± 0.175
0.768TrpSer: 0.768 ± 0.25
0.256TrpThr: 0.256 ± 0.147
0.853TrpVal: 0.853 ± 0.222
0.085TrpTrp: 0.085 ± 0.094
0.682TrpTyr: 0.682 ± 0.222
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.365TyrAla: 1.365 ± 0.385
0.768TyrCys: 0.768 ± 0.272
3.156TyrAsp: 3.156 ± 0.609
2.815TyrGlu: 2.815 ± 0.409
1.45TyrPhe: 1.45 ± 0.343
2.133TyrGly: 2.133 ± 0.431
0.427TyrHis: 0.427 ± 0.192
2.815TyrIle: 2.815 ± 0.46
3.497TyrLys: 3.497 ± 0.436
2.73TyrLeu: 2.73 ± 0.425
0.853TyrMet: 0.853 ± 0.293
1.365TyrAsn: 1.365 ± 0.388
1.109TyrPro: 1.109 ± 0.334
1.28TyrGln: 1.28 ± 0.304
2.133TyrArg: 2.133 ± 0.366
1.962TyrSer: 1.962 ± 0.406
2.047TyrThr: 2.047 ± 0.403
2.986TyrVal: 2.986 ± 0.565
0.597TyrTrp: 0.597 ± 0.213
1.791TyrTyr: 1.791 ± 0.384
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (11724 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski