Clostridium phage phiMMP04

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Sherbrookevirus; Clostridioides virus phiMMP04

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9QD33|J9QD33_9CAUD Uncharacterized protein OS=Clostridium phage phiMMP04 OX=1204535 GN=phiMMP04_gp40 PE=4 SV=1
MM1 pKa = 7.89KK2 pKa = 10.36LRR4 pKa = 11.84IGIIDD9 pKa = 3.85KK10 pKa = 10.76YY11 pKa = 10.26VAEE14 pKa = 4.44ASYY17 pKa = 10.7EE18 pKa = 4.0LSEE21 pKa = 4.67LLIDD25 pKa = 5.05AFNKK29 pKa = 9.84YY30 pKa = 10.73DD31 pKa = 3.75EE32 pKa = 4.5FVEE35 pKa = 4.85AIRR38 pKa = 11.84GGEE41 pKa = 3.67IRR43 pKa = 11.84IVPFNLGFQALLEE56 pKa = 4.34EE57 pKa = 5.12DD58 pKa = 3.61STYY61 pKa = 11.4DD62 pKa = 3.08EE63 pKa = 4.77YY64 pKa = 11.59EE65 pKa = 3.98FYY67 pKa = 11.23YY68 pKa = 10.84DD69 pKa = 6.23DD70 pKa = 5.51EE71 pKa = 5.82DD72 pKa = 4.27DD73 pKa = 3.76KK74 pKa = 11.87CFFHH78 pKa = 7.2IKK80 pKa = 9.21YY81 pKa = 10.43RR82 pKa = 11.84NVKK85 pKa = 10.1DD86 pKa = 3.64VEE88 pKa = 4.2LSKK91 pKa = 11.02EE92 pKa = 3.99LLLEE96 pKa = 4.7LIKK99 pKa = 11.33VNLPAGAKK107 pKa = 10.32LIVNNN112 pKa = 4.49

Molecular weight: 13.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9QD32|J9QD32_9CAUD Uncharacterized protein OS=Clostridium phage phiMMP04 OX=1204535 GN=phiMMP04_gp35 PE=4 SV=1
MM1 pKa = 7.39GRR3 pKa = 11.84LEE5 pKa = 3.89RR6 pKa = 11.84SKK8 pKa = 10.98KK9 pKa = 9.45KK10 pKa = 10.47RR11 pKa = 11.84EE12 pKa = 3.74NKK14 pKa = 9.71FNIVKK19 pKa = 10.27KK20 pKa = 9.83VFSFILLLLNIILAILRR37 pKa = 11.84ILKK40 pKa = 9.69EE41 pKa = 3.88LL42 pKa = 3.72

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

9084

34

718

178.1

20.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.273 ± 0.38

1.035 ± 0.179

5.801 ± 0.24

9.236 ± 0.547

3.974 ± 0.221

5.427 ± 0.411

1.068 ± 0.139

9.368 ± 0.306

10.777 ± 0.407

8.432 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.229

6.495 ± 0.347

2.092 ± 0.181

2.345 ± 0.162

3.699 ± 0.198

6.11 ± 0.411

5.636 ± 0.427

5.812 ± 0.268

0.881 ± 0.104

4.062 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski