Bacillus phage Basilisk

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 138 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5MLZ0|S5MLZ0_9CAUD Uncharacterized protein OS=Bacillus phage Basilisk OX=1296654 GN=BASILISK_4 PE=4 SV=1
MM1 pKa = 7.49YY2 pKa = 10.78NMKK5 pKa = 10.4VKK7 pKa = 9.45FTAKK11 pKa = 10.53YY12 pKa = 9.01EE13 pKa = 4.13NKK15 pKa = 10.12EE16 pKa = 4.32DD17 pKa = 3.57GLKK20 pKa = 10.36AYY22 pKa = 9.78EE23 pKa = 5.02FEE25 pKa = 4.44TPDD28 pKa = 4.15YY29 pKa = 10.04IQNDD33 pKa = 3.0IDD35 pKa = 4.9GYY37 pKa = 10.45SSDD40 pKa = 4.76IFRR43 pKa = 11.84LDD45 pKa = 3.53CLKK48 pKa = 10.9GEE50 pKa = 4.06WMILEE55 pKa = 4.38RR56 pKa = 11.84MSDD59 pKa = 3.31APYY62 pKa = 9.75MVSEE66 pKa = 4.61DD67 pKa = 3.54VTHH70 pKa = 7.13LFNLEE75 pKa = 4.44DD76 pKa = 5.06IIKK79 pKa = 10.12QMQDD83 pKa = 2.93VEE85 pKa = 4.98SEE87 pKa = 4.26IPCEE91 pKa = 3.83QEE93 pKa = 5.78EE94 pKa = 4.13YY95 pKa = 11.09DD96 pKa = 4.18EE97 pKa = 4.12WWEE100 pKa = 5.15DD101 pKa = 3.16EE102 pKa = 4.28GQYY105 pKa = 10.24EE106 pKa = 4.19

Molecular weight: 12.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5MSD7|S5MSD7_9CAUD Uncharacterized protein OS=Bacillus phage Basilisk OX=1296654 GN=BASILISK_29 PE=4 SV=1
MM1 pKa = 7.55IGNRR5 pKa = 11.84WVSNPKK11 pKa = 9.95RR12 pKa = 11.84KK13 pKa = 7.4QTRR16 pKa = 11.84ICFFMAHH23 pKa = 5.55TSMFLTGAYY32 pKa = 9.47VPKK35 pKa = 10.14EE36 pKa = 3.95PNRR39 pKa = 11.84VGMILLFCRR48 pKa = 11.84THH50 pKa = 6.34NLSTT54 pKa = 3.72

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

138

0

138

25117

24

2185

182.0

20.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.753 ± 0.417

0.876 ± 0.104

6.346 ± 0.166

7.624 ± 0.337

4.407 ± 0.168

6.287 ± 0.284

1.708 ± 0.153

7.035 ± 0.212

8.89 ± 0.333

7.819 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.046 ± 0.141

6.084 ± 0.189

2.64 ± 0.156

3.599 ± 0.349

3.87 ± 0.149

5.435 ± 0.261

6.235 ± 0.317

6.645 ± 0.18

1.202 ± 0.087

4.499 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski