Capybara microvirus Cap1_SP_116

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4V1FVP0|A0A4V1FVP0_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_116 OX=2584809 PE=4 SV=1
MM1 pKa = 8.11PDD3 pKa = 3.69LSNHH7 pKa = 6.7AGSQVTPNPVTSGPYY22 pKa = 9.76SVVNTADD29 pKa = 3.86QLSGWNRR36 pKa = 11.84LTDD39 pKa = 3.42WLGVTNHH46 pKa = 5.99QGQINNQLALQQSLLDD62 pKa = 4.7DD63 pKa = 3.53EE64 pKa = 5.46RR65 pKa = 11.84AYY67 pKa = 11.25NDD69 pKa = 3.35PAAQVEE75 pKa = 4.27RR76 pKa = 11.84LRR78 pKa = 11.84NAGINPDD85 pKa = 3.6LLGGVSSYY93 pKa = 11.44EE94 pKa = 4.11STGQGANPLSQPTGDD109 pKa = 3.58FGNFILGVAQTFSGVASGILDD130 pKa = 4.08LQSKK134 pKa = 10.46ALDD137 pKa = 3.64NDD139 pKa = 3.46IKK141 pKa = 10.74RR142 pKa = 11.84ASLFDD147 pKa = 3.51TEE149 pKa = 5.41SILNRR154 pKa = 11.84IASDD158 pKa = 3.48RR159 pKa = 11.84SISADD164 pKa = 3.79FIEE167 pKa = 5.59AMKK170 pKa = 10.73DD171 pKa = 3.34PNRR174 pKa = 11.84LKK176 pKa = 10.66PAVSLDD182 pKa = 3.28QYY184 pKa = 11.62VNLSTIRR191 pKa = 11.84SKK193 pKa = 10.8KK194 pKa = 8.03VRR196 pKa = 11.84KK197 pKa = 9.28AVEE200 pKa = 3.87DD201 pKa = 3.48NFYY204 pKa = 11.35SKK206 pKa = 10.77LYY208 pKa = 10.61SDD210 pKa = 6.04DD211 pKa = 4.12FIQRR215 pKa = 11.84INGLLTGVNKK225 pKa = 10.01SEE227 pKa = 4.33RR228 pKa = 11.84DD229 pKa = 3.45FSEE232 pKa = 4.31SVAGLRR238 pKa = 11.84NPSKK242 pKa = 9.73TADD245 pKa = 3.64SLIDD249 pKa = 3.52ALAEE253 pKa = 4.19FMSDD257 pKa = 3.3AYY259 pKa = 7.52TTQMSANKK267 pKa = 10.14AEE269 pKa = 4.13RR270 pKa = 11.84HH271 pKa = 6.28HH272 pKa = 6.77NDD274 pKa = 2.94ASISQDD280 pKa = 3.63DD281 pKa = 4.16YY282 pKa = 11.99NKK284 pKa = 9.92MVTDD288 pKa = 4.88ALDD291 pKa = 3.78PSNEE295 pKa = 3.9SDD297 pKa = 3.94AITAGFEE304 pKa = 3.89ASEE307 pKa = 4.26TSKK310 pKa = 10.86KK311 pKa = 9.68KK312 pKa = 10.42QSNIDD317 pKa = 3.55SVIEE321 pKa = 3.98EE322 pKa = 4.38FTGKK326 pKa = 8.8MKK328 pKa = 10.83KK329 pKa = 9.82KK330 pKa = 9.27DD331 pKa = 3.65TWWSDD336 pKa = 3.64LILAIMAMYY345 pKa = 10.32QSGAFDD351 pKa = 3.22SFGFSRR357 pKa = 11.84SSQSSFTPRR366 pKa = 11.84GGSQEE371 pKa = 3.49RR372 pKa = 11.84SSFNFHH378 pKa = 6.92LL379 pKa = 4.6

Molecular weight:
41.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4P8W461|A0A4P8W461_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_116 OX=2584809 PE=4 SV=1
MM1 pKa = 7.64SILALILSLVSLIRR15 pKa = 11.84TGRR18 pKa = 11.84NHH20 pKa = 7.12RR21 pKa = 11.84DD22 pKa = 2.93VSRR25 pKa = 11.84LVDD28 pKa = 3.77TVAHH32 pKa = 6.18IEE34 pKa = 3.8EE35 pKa = 4.75SIRR38 pKa = 11.84VNDD41 pKa = 3.5QAFVDD46 pKa = 3.81ACKK49 pKa = 10.75SFTEE53 pKa = 4.6SIEE56 pKa = 4.06KK57 pKa = 10.26LDD59 pKa = 3.73YY60 pKa = 11.07ALVMLEE66 pKa = 4.8RR67 pKa = 11.84YY68 pKa = 6.69TLPSGSSSLIVTGPRR83 pKa = 11.84SKK85 pKa = 10.01VTTTDD90 pKa = 3.06SEE92 pKa = 5.49SNMSVSDD99 pKa = 3.88SRR101 pKa = 11.84NQSSDD106 pKa = 3.04KK107 pKa = 10.62KK108 pKa = 10.99SKK110 pKa = 10.69NKK112 pKa = 9.97NN113 pKa = 3.06

Molecular weight:
12.49 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1529

113

630

382.3

43.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.971 ± 1.451

1.57 ± 0.66

7.456 ± 0.709

3.924 ± 0.327

4.84 ± 0.479

5.298 ± 0.449

2.42 ± 0.371

5.298 ± 0.529

4.971 ± 1.105

8.698 ± 0.199

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.962 ± 0.121

6.802 ± 0.563

4.186 ± 0.732

4.382 ± 0.568

4.971 ± 0.443

11.445 ± 1.123

4.774 ± 0.854

5.755 ± 0.533

1.046 ± 0.182

5.232 ± 1.088

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski