Fusobacterium necrophorum subsp. funduliforme 1_1_36S

Taxonomy: cellular organisms; Bacteria; Fusobacteria; Fusobacteriia; Fusobacteriales; Fusobacteriaceae; Fusobacterium; Fusobacterium necrophorum; Fusobacterium necrophorum subsp. funduliforme

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H1D774|H1D774_9FUSO Bro-N domain-containing protein OS=Fusobacterium necrophorum subsp. funduliforme 1_1_36S OX=742814 GN=HMPREF9466_01570 PE=4 SV=1
MM1 pKa = 8.01PYY3 pKa = 9.72MYY5 pKa = 10.55FDD7 pKa = 6.36DD8 pKa = 5.89DD9 pKa = 3.88LQKK12 pKa = 11.23YY13 pKa = 10.07IIVEE17 pKa = 4.06NEE19 pKa = 3.51KK20 pKa = 10.85VIYY23 pKa = 8.5EE24 pKa = 4.32TEE26 pKa = 4.19DD27 pKa = 3.13EE28 pKa = 4.6SNAFLVLDD36 pKa = 4.86DD37 pKa = 4.61LTKK40 pKa = 10.96EE41 pKa = 4.21DD42 pKa = 3.66

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H1D609|H1D609_9FUSO Uncharacterized protein OS=Fusobacterium necrophorum subsp. funduliforme 1_1_36S OX=742814 GN=HMPREF9466_00892 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.34FGFSNIIILVILEE15 pKa = 3.93KK16 pKa = 9.94WDD18 pKa = 3.18NRR20 pKa = 11.84MAFEE24 pKa = 4.11VLLLRR29 pKa = 11.84IFIQALMLGTMFSPGFLVSLCSGFFSLCLTTILYY63 pKa = 8.74RR64 pKa = 11.84ARR66 pKa = 11.84RR67 pKa = 11.84YY68 pKa = 10.37LSIISISCLSATFHH82 pKa = 6.73NVVQLLVVYY91 pKa = 9.29FLLFRR96 pKa = 11.84NIALQSKK103 pKa = 10.22SIMIFIFGFLLLGVLSGLITGVFVTKK129 pKa = 10.6LRR131 pKa = 11.84LRR133 pKa = 11.84VPNSKK138 pKa = 10.08EE139 pKa = 3.65KK140 pKa = 10.34RR141 pKa = 11.84RR142 pKa = 11.84KK143 pKa = 9.34QSLL146 pKa = 3.3

Molecular weight: 16.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3196

0

3196

671340

29

1303

210.1

23.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.023 ± 0.058

1.002 ± 0.019

4.549 ± 0.038

8.543 ± 0.061

4.917 ± 0.046

6.59 ± 0.051

1.748 ± 0.021

8.426 ± 0.048

8.861 ± 0.044

9.596 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.829 ± 0.024

4.797 ± 0.04

2.812 ± 0.026

3.327 ± 0.027

4.085 ± 0.034

6.155 ± 0.037

4.645 ± 0.032

6.098 ± 0.041

0.829 ± 0.016

4.169 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski