Amino acid dipepetide frequency for Clostridium phage phiMMP02

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.402AlaAla: 1.402 ± 0.354
0.35AlaCys: 0.35 ± 0.171
2.803AlaAsp: 2.803 ± 0.422
3.995AlaGlu: 3.995 ± 0.479
1.682AlaPhe: 1.682 ± 0.546
3.574AlaGly: 3.574 ± 0.827
0.35AlaHis: 0.35 ± 0.135
4.766AlaIle: 4.766 ± 0.918
5.607AlaLys: 5.607 ± 0.611
5.607AlaLeu: 5.607 ± 0.705
1.822AlaMet: 1.822 ± 0.388
2.523AlaAsn: 2.523 ± 0.388
0.841AlaPro: 0.841 ± 0.241
1.472AlaGln: 1.472 ± 0.309
2.313AlaArg: 2.313 ± 0.399
4.696AlaSer: 4.696 ± 0.993
4.696AlaThr: 4.696 ± 1.016
3.084AlaVal: 3.084 ± 0.423
0.35AlaTrp: 0.35 ± 0.129
1.962AlaTyr: 1.962 ± 0.472
0.0AlaXaa: 0.0 ± 0.0
Cys
0.421CysAla: 0.421 ± 0.186
0.21CysCys: 0.21 ± 0.106
0.841CysAsp: 0.841 ± 0.292
0.841CysGlu: 0.841 ± 0.247
0.35CysPhe: 0.35 ± 0.195
0.561CysGly: 0.561 ± 0.146
0.14CysHis: 0.14 ± 0.108
1.262CysIle: 1.262 ± 0.27
0.981CysLys: 0.981 ± 0.258
0.631CysLeu: 0.631 ± 0.223
0.21CysMet: 0.21 ± 0.132
0.421CysAsn: 0.421 ± 0.162
0.14CysPro: 0.14 ± 0.099
0.21CysGln: 0.21 ± 0.137
0.561CysArg: 0.561 ± 0.198
0.631CysSer: 0.631 ± 0.212
0.701CysThr: 0.701 ± 0.19
0.491CysVal: 0.491 ± 0.195
0.21CysTrp: 0.21 ± 0.114
0.561CysTyr: 0.561 ± 0.172
0.0CysXaa: 0.0 ± 0.0
Asp
2.453AspAla: 2.453 ± 0.325
0.771AspCys: 0.771 ± 0.268
3.574AspAsp: 3.574 ± 0.623
5.397AspGlu: 5.397 ± 0.633
2.593AspPhe: 2.593 ± 0.426
3.294AspGly: 3.294 ± 0.581
0.14AspHis: 0.14 ± 0.081
4.976AspIle: 4.976 ± 0.514
5.607AspLys: 5.607 ± 0.829
4.415AspLeu: 4.415 ± 0.387
1.752AspMet: 1.752 ± 0.296
5.046AspAsn: 5.046 ± 0.521
0.911AspPro: 0.911 ± 0.268
0.771AspGln: 0.771 ± 0.234
2.453AspArg: 2.453 ± 0.486
3.434AspSer: 3.434 ± 0.44
2.944AspThr: 2.944 ± 0.487
3.645AspVal: 3.645 ± 0.411
0.35AspTrp: 0.35 ± 0.152
2.103AspTyr: 2.103 ± 0.376
0.0AspXaa: 0.0 ± 0.0
Glu
4.976GluAla: 4.976 ± 0.589
0.841GluCys: 0.841 ± 0.226
4.205GluAsp: 4.205 ± 0.595
7.219GluGlu: 7.219 ± 0.956
3.645GluPhe: 3.645 ± 0.587
3.715GluGly: 3.715 ± 0.485
0.561GluHis: 0.561 ± 0.18
7.71GluIle: 7.71 ± 0.988
8.34GluLys: 8.34 ± 0.93
8.2GluLeu: 8.2 ± 0.869
3.154GluMet: 3.154 ± 0.657
6.378GluAsn: 6.378 ± 0.735
1.402GluPro: 1.402 ± 0.345
3.645GluGln: 3.645 ± 0.517
3.084GluArg: 3.084 ± 0.596
3.504GluSer: 3.504 ± 0.441
5.327GluThr: 5.327 ± 0.586
5.467GluVal: 5.467 ± 0.777
0.841GluTrp: 0.841 ± 0.268
3.645GluTyr: 3.645 ± 0.497
0.0GluXaa: 0.0 ± 0.0
Phe
2.033PheAla: 2.033 ± 0.423
0.28PheCys: 0.28 ± 0.133
1.892PheAsp: 1.892 ± 0.311
3.434PheGlu: 3.434 ± 0.553
1.191PhePhe: 1.191 ± 0.271
2.803PheGly: 2.803 ± 0.409
0.421PheHis: 0.421 ± 0.161
3.785PheIle: 3.785 ± 0.549
4.205PheLys: 4.205 ± 0.479
2.523PheLeu: 2.523 ± 0.421
0.561PheMet: 0.561 ± 0.188
2.874PheAsn: 2.874 ± 0.473
1.121PhePro: 1.121 ± 0.295
0.771PheGln: 0.771 ± 0.207
1.472PheArg: 1.472 ± 0.408
1.892PheSer: 1.892 ± 0.341
2.313PheThr: 2.313 ± 0.333
1.892PheVal: 1.892 ± 0.335
0.21PheTrp: 0.21 ± 0.13
1.402PheTyr: 1.402 ± 0.304
0.0PheXaa: 0.0 ± 0.0
Gly
3.224GlyAla: 3.224 ± 0.718
0.841GlyCys: 0.841 ± 0.255
2.103GlyAsp: 2.103 ± 0.378
6.308GlyGlu: 6.308 ± 0.797
3.364GlyPhe: 3.364 ± 0.446
5.186GlyGly: 5.186 ± 1.635
0.841GlyHis: 0.841 ± 0.293
5.046GlyIle: 5.046 ± 0.647
5.186GlyLys: 5.186 ± 0.521
4.275GlyLeu: 4.275 ± 0.644
2.033GlyMet: 2.033 ± 0.386
3.294GlyAsn: 3.294 ± 0.461
0.561GlyPro: 0.561 ± 0.269
1.402GlyGln: 1.402 ± 0.389
2.033GlyArg: 2.033 ± 0.378
4.065GlySer: 4.065 ± 0.555
3.154GlyThr: 3.154 ± 0.643
4.135GlyVal: 4.135 ± 0.76
0.771GlyTrp: 0.771 ± 0.176
2.383GlyTyr: 2.383 ± 0.542
0.0GlyXaa: 0.0 ± 0.0
His
0.21HisAla: 0.21 ± 0.118
0.28HisCys: 0.28 ± 0.135
0.35HisAsp: 0.35 ± 0.157
0.841HisGlu: 0.841 ± 0.248
0.631HisPhe: 0.631 ± 0.206
0.35HisGly: 0.35 ± 0.171
0.07HisHis: 0.07 ± 0.079
0.561HisIle: 0.561 ± 0.192
0.981HisLys: 0.981 ± 0.294
0.771HisLeu: 0.771 ± 0.237
0.28HisMet: 0.28 ± 0.136
0.491HisAsn: 0.491 ± 0.149
0.491HisPro: 0.491 ± 0.226
0.21HisGln: 0.21 ± 0.127
0.21HisArg: 0.21 ± 0.109
0.631HisSer: 0.631 ± 0.196
0.771HisThr: 0.771 ± 0.241
0.35HisVal: 0.35 ± 0.165
0.21HisTrp: 0.21 ± 0.115
0.35HisTyr: 0.35 ± 0.156
0.0HisXaa: 0.0 ± 0.0
Ile
4.766IleAla: 4.766 ± 0.543
1.402IleCys: 1.402 ± 0.397
6.378IleAsp: 6.378 ± 0.676
6.939IleGlu: 6.939 ± 0.771
2.523IlePhe: 2.523 ± 0.419
5.116IleGly: 5.116 ± 0.555
0.841IleHis: 0.841 ± 0.251
5.957IleIle: 5.957 ± 0.644
8.831IleLys: 8.831 ± 0.748
7.429IleLeu: 7.429 ± 0.789
1.822IleMet: 1.822 ± 0.357
5.747IleAsn: 5.747 ± 0.579
2.313IlePro: 2.313 ± 0.487
2.874IleGln: 2.874 ± 0.451
4.065IleArg: 4.065 ± 0.522
5.537IleSer: 5.537 ± 0.542
4.766IleThr: 4.766 ± 0.548
3.995IleVal: 3.995 ± 0.523
0.771IleTrp: 0.771 ± 0.265
3.154IleTyr: 3.154 ± 0.486
0.0IleXaa: 0.0 ± 0.0
Lys
6.448LysAla: 6.448 ± 0.889
0.911LysCys: 0.911 ± 0.274
6.728LysAsp: 6.728 ± 0.789
11.284LysGlu: 11.284 ± 0.944
3.294LysPhe: 3.294 ± 0.464
6.027LysGly: 6.027 ± 0.556
0.841LysHis: 0.841 ± 0.263
8.691LysIle: 8.691 ± 0.875
11.074LysLys: 11.074 ± 1.3
7.71LysLeu: 7.71 ± 0.801
3.014LysMet: 3.014 ± 0.47
7.71LysAsn: 7.71 ± 0.663
2.173LysPro: 2.173 ± 0.403
3.925LysGln: 3.925 ± 0.502
3.925LysArg: 3.925 ± 0.546
6.378LysSer: 6.378 ± 0.831
5.116LysThr: 5.116 ± 0.567
6.168LysVal: 6.168 ± 0.544
0.911LysTrp: 0.911 ± 0.266
4.275LysTyr: 4.275 ± 0.46
0.0LysXaa: 0.0 ± 0.0
Leu
4.415LeuAla: 4.415 ± 0.591
0.981LeuCys: 0.981 ± 0.267
5.257LeuAsp: 5.257 ± 0.581
7.71LeuGlu: 7.71 ± 0.781
2.663LeuPhe: 2.663 ± 0.472
5.327LeuGly: 5.327 ± 0.804
0.631LeuHis: 0.631 ± 0.222
5.537LeuIle: 5.537 ± 0.546
10.934LeuLys: 10.934 ± 0.795
6.027LeuLeu: 6.027 ± 0.638
1.542LeuMet: 1.542 ± 0.322
6.869LeuAsn: 6.869 ± 0.728
1.682LeuPro: 1.682 ± 0.334
2.523LeuGln: 2.523 ± 0.41
2.874LeuArg: 2.874 ± 0.441
5.747LeuSer: 5.747 ± 0.564
5.046LeuThr: 5.046 ± 0.596
3.995LeuVal: 3.995 ± 0.604
0.28LeuTrp: 0.28 ± 0.133
2.944LeuTyr: 2.944 ± 0.448
0.0LeuXaa: 0.0 ± 0.0
Met
1.612MetAla: 1.612 ± 0.361
0.21MetCys: 0.21 ± 0.117
1.332MetAsp: 1.332 ± 0.242
1.612MetGlu: 1.612 ± 0.36
0.561MetPhe: 0.561 ± 0.182
1.051MetGly: 1.051 ± 0.298
0.21MetHis: 0.21 ± 0.108
2.033MetIle: 2.033 ± 0.382
3.014MetLys: 3.014 ± 0.406
2.803MetLeu: 2.803 ± 0.357
0.28MetMet: 0.28 ± 0.14
1.682MetAsn: 1.682 ± 0.334
0.631MetPro: 0.631 ± 0.175
0.841MetGln: 0.841 ± 0.203
0.561MetArg: 0.561 ± 0.172
2.103MetSer: 2.103 ± 0.442
1.542MetThr: 1.542 ± 0.349
0.771MetVal: 0.771 ± 0.241
0.35MetTrp: 0.35 ± 0.143
1.402MetTyr: 1.402 ± 0.262
0.0MetXaa: 0.0 ± 0.0
Asn
4.486AsnAla: 4.486 ± 0.637
0.631AsnCys: 0.631 ± 0.237
3.995AsnAsp: 3.995 ± 0.641
5.116AsnGlu: 5.116 ± 0.766
2.944AsnPhe: 2.944 ± 0.531
5.186AsnGly: 5.186 ± 0.604
0.35AsnHis: 0.35 ± 0.146
6.939AsnIle: 6.939 ± 0.823
7.99AsnLys: 7.99 ± 0.652
5.747AsnLeu: 5.747 ± 0.602
1.892AsnMet: 1.892 ± 0.315
4.626AsnAsn: 4.626 ± 0.648
1.472AsnPro: 1.472 ± 0.344
1.822AsnGln: 1.822 ± 0.308
2.803AsnArg: 2.803 ± 0.382
4.205AsnSer: 4.205 ± 0.66
3.995AsnThr: 3.995 ± 0.514
3.995AsnVal: 3.995 ± 0.531
0.911AsnTrp: 0.911 ± 0.273
1.542AsnTyr: 1.542 ± 0.31
0.0AsnXaa: 0.0 ± 0.0
Pro
0.701ProAla: 0.701 ± 0.231
0.35ProCys: 0.35 ± 0.14
0.981ProAsp: 0.981 ± 0.233
1.121ProGlu: 1.121 ± 0.284
0.631ProPhe: 0.631 ± 0.196
0.981ProGly: 0.981 ± 0.282
0.421ProHis: 0.421 ± 0.182
2.243ProIle: 2.243 ± 0.395
2.453ProLys: 2.453 ± 0.446
1.332ProLeu: 1.332 ± 0.336
0.21ProMet: 0.21 ± 0.087
1.892ProAsn: 1.892 ± 0.34
0.561ProPro: 0.561 ± 0.197
1.332ProGln: 1.332 ± 0.28
0.841ProArg: 0.841 ± 0.239
0.771ProSer: 0.771 ± 0.176
2.033ProThr: 2.033 ± 0.326
1.262ProVal: 1.262 ± 0.271
0.07ProTrp: 0.07 ± 0.066
0.631ProTyr: 0.631 ± 0.21
0.0ProXaa: 0.0 ± 0.0
Gln
2.523GlnAla: 2.523 ± 0.716
0.07GlnCys: 0.07 ± 0.067
1.892GlnAsp: 1.892 ± 0.295
3.084GlnGlu: 3.084 ± 0.538
1.191GlnPhe: 1.191 ± 0.242
2.173GlnGly: 2.173 ± 0.345
0.14GlnHis: 0.14 ± 0.102
2.803GlnIle: 2.803 ± 0.424
2.523GlnLys: 2.523 ± 0.483
3.224GlnLeu: 3.224 ± 0.517
0.771GlnMet: 0.771 ± 0.187
2.243GlnAsn: 2.243 ± 0.351
0.491GlnPro: 0.491 ± 0.195
1.402GlnGln: 1.402 ± 0.445
0.631GlnArg: 0.631 ± 0.181
1.962GlnSer: 1.962 ± 0.379
2.313GlnThr: 2.313 ± 0.412
1.262GlnVal: 1.262 ± 0.302
0.14GlnTrp: 0.14 ± 0.092
1.051GlnTyr: 1.051 ± 0.253
0.0GlnXaa: 0.0 ± 0.0
Arg
1.752ArgAla: 1.752 ± 0.305
0.35ArgCys: 0.35 ± 0.163
1.542ArgAsp: 1.542 ± 0.369
3.785ArgGlu: 3.785 ± 0.474
0.771ArgPhe: 0.771 ± 0.207
2.103ArgGly: 2.103 ± 0.393
0.491ArgHis: 0.491 ± 0.177
3.715ArgIle: 3.715 ± 0.525
3.645ArgLys: 3.645 ± 0.614
3.995ArgLeu: 3.995 ± 0.634
1.121ArgMet: 1.121 ± 0.275
1.332ArgAsn: 1.332 ± 0.315
0.911ArgPro: 0.911 ± 0.271
1.051ArgGln: 1.051 ± 0.227
1.402ArgArg: 1.402 ± 0.381
1.612ArgSer: 1.612 ± 0.32
2.103ArgThr: 2.103 ± 0.417
3.084ArgVal: 3.084 ± 0.389
0.561ArgTrp: 0.561 ± 0.159
1.191ArgTyr: 1.191 ± 0.24
0.0ArgXaa: 0.0 ± 0.0
Ser
3.014SerAla: 3.014 ± 0.977
0.491SerCys: 0.491 ± 0.175
3.785SerAsp: 3.785 ± 0.473
4.345SerGlu: 4.345 ± 0.519
3.154SerPhe: 3.154 ± 0.527
3.014SerGly: 3.014 ± 0.468
0.771SerHis: 0.771 ± 0.291
5.186SerIle: 5.186 ± 0.583
7.219SerLys: 7.219 ± 0.802
4.135SerLeu: 4.135 ± 0.696
1.402SerMet: 1.402 ± 0.377
5.257SerAsn: 5.257 ± 0.626
0.981SerPro: 0.981 ± 0.234
2.243SerGln: 2.243 ± 0.458
1.682SerArg: 1.682 ± 0.289
5.257SerSer: 5.257 ± 1.142
3.364SerThr: 3.364 ± 0.435
3.574SerVal: 3.574 ± 0.559
0.491SerTrp: 0.491 ± 0.149
2.803SerTyr: 2.803 ± 0.491
0.0SerXaa: 0.0 ± 0.0
Thr
4.556ThrAla: 4.556 ± 0.971
0.21ThrCys: 0.21 ± 0.125
3.084ThrAsp: 3.084 ± 0.489
3.504ThrGlu: 3.504 ± 0.544
2.313ThrPhe: 2.313 ± 0.353
3.785ThrGly: 3.785 ± 0.533
0.841ThrHis: 0.841 ± 0.228
5.467ThrIle: 5.467 ± 0.587
6.588ThrLys: 6.588 ± 0.551
5.677ThrLeu: 5.677 ± 0.57
0.911ThrMet: 0.911 ± 0.236
3.434ThrAsn: 3.434 ± 0.479
2.243ThrPro: 2.243 ± 0.34
2.453ThrGln: 2.453 ± 0.451
1.822ThrArg: 1.822 ± 0.299
3.715ThrSer: 3.715 ± 0.609
3.925ThrThr: 3.925 ± 0.611
3.925ThrVal: 3.925 ± 0.55
0.35ThrTrp: 0.35 ± 0.167
1.962ThrTyr: 1.962 ± 0.374
0.0ThrXaa: 0.0 ± 0.0
Val
3.224ValAla: 3.224 ± 0.462
0.421ValCys: 0.421 ± 0.173
2.944ValAsp: 2.944 ± 0.565
5.186ValGlu: 5.186 ± 0.623
1.472ValPhe: 1.472 ± 0.267
3.785ValGly: 3.785 ± 0.569
0.491ValHis: 0.491 ± 0.153
4.486ValIle: 4.486 ± 0.604
5.607ValLys: 5.607 ± 0.649
4.626ValLeu: 4.626 ± 0.495
0.911ValMet: 0.911 ± 0.167
5.327ValAsn: 5.327 ± 0.706
0.771ValPro: 0.771 ± 0.195
1.402ValGln: 1.402 ± 0.326
2.383ValArg: 2.383 ± 0.348
3.645ValSer: 3.645 ± 0.489
3.925ValThr: 3.925 ± 0.579
4.275ValVal: 4.275 ± 0.599
0.771ValTrp: 0.771 ± 0.26
2.173ValTyr: 2.173 ± 0.492
0.0ValXaa: 0.0 ± 0.0
Trp
0.561TrpAla: 0.561 ± 0.187
0.14TrpCys: 0.14 ± 0.102
0.701TrpAsp: 0.701 ± 0.204
0.491TrpGlu: 0.491 ± 0.185
0.491TrpPhe: 0.491 ± 0.206
0.561TrpGly: 0.561 ± 0.197
0.0TrpHis: 0.0 ± 0.0
0.841TrpIle: 0.841 ± 0.266
0.631TrpLys: 0.631 ± 0.204
0.701TrpLeu: 0.701 ± 0.211
0.14TrpMet: 0.14 ± 0.107
0.771TrpAsn: 0.771 ± 0.209
0.21TrpPro: 0.21 ± 0.124
0.701TrpGln: 0.701 ± 0.202
0.35TrpArg: 0.35 ± 0.141
0.21TrpSer: 0.21 ± 0.123
0.491TrpThr: 0.491 ± 0.189
0.701TrpVal: 0.701 ± 0.198
0.07TrpTrp: 0.07 ± 0.066
0.35TrpTyr: 0.35 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.121TyrAla: 1.121 ± 0.296
0.631TyrCys: 0.631 ± 0.198
2.243TyrAsp: 2.243 ± 0.428
3.645TyrGlu: 3.645 ± 0.547
1.612TyrPhe: 1.612 ± 0.416
1.612TyrGly: 1.612 ± 0.345
0.491TyrHis: 0.491 ± 0.18
3.224TyrIle: 3.224 ± 0.57
5.186TyrLys: 5.186 ± 0.763
3.154TyrLeu: 3.154 ± 0.527
0.701TyrMet: 0.701 ± 0.204
2.733TyrAsn: 2.733 ± 0.372
0.911TyrPro: 0.911 ± 0.273
0.841TyrGln: 0.841 ± 0.256
1.051TyrArg: 1.051 ± 0.222
2.243TyrSer: 2.243 ± 0.422
2.243TyrThr: 2.243 ± 0.438
1.752TyrVal: 1.752 ± 0.28
0.491TyrTrp: 0.491 ± 0.183
1.892TyrTyr: 1.892 ± 0.427
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (14269 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski