Bacillus phage JL

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Spounavirinae; Siminovitchvirus; Bacillus virus JL

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5MM31|S5MM31_9CAUD Uncharacterized protein OS=Bacillus phage JL OX=1296655 GN=JL_35 PE=4 SV=1
MM1 pKa = 7.34SEE3 pKa = 4.43KK4 pKa = 10.17ISVYY8 pKa = 9.29MPEE11 pKa = 3.9PHH13 pKa = 6.94HH14 pKa = 6.61EE15 pKa = 4.16EE16 pKa = 4.16GVPAVYY22 pKa = 9.96LVHH25 pKa = 7.23GIDD28 pKa = 4.29AVQLSDD34 pKa = 6.05LIGQFIGAVEE44 pKa = 4.07EE45 pKa = 4.19HH46 pKa = 7.33DD47 pKa = 5.85EE48 pKa = 4.15DD49 pKa = 6.19AEE51 pKa = 4.22WCTDD55 pKa = 3.36DD56 pKa = 4.56VVGHH60 pKa = 6.33LRR62 pKa = 11.84EE63 pKa = 3.84QGYY66 pKa = 7.57TVTKK70 pKa = 9.77PGVVCADD77 pKa = 3.79YY78 pKa = 11.42EE79 pKa = 4.43DD80 pKa = 5.17GSWIEE85 pKa = 4.05QDD87 pKa = 3.65SEE89 pKa = 4.19

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5M4S5|S5M4S5_9CAUD Uncharacterized protein OS=Bacillus phage JL OX=1296655 GN=JL_217 PE=4 SV=1
MM1 pKa = 8.16DD2 pKa = 5.48KK3 pKa = 9.97NTMGGLARR11 pKa = 11.84ALRR14 pKa = 11.84NLHH17 pKa = 6.12YY18 pKa = 10.36YY19 pKa = 9.09EE20 pKa = 4.37KK21 pKa = 10.46SGRR24 pKa = 11.84NPVLSPFSLIRR35 pKa = 11.84RR36 pKa = 11.84LFIGG40 pKa = 3.76

Molecular weight: 4.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

218

0

218

42734

19

3601

196.0

22.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.079 ± 0.225

1.025 ± 0.086

6.522 ± 0.187

7.673 ± 0.298

3.442 ± 0.094

7.097 ± 0.258

2.256 ± 0.177

6.339 ± 0.179

8.078 ± 0.246

7.816 ± 0.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.246 ± 0.169

4.884 ± 0.223

3.599 ± 0.137

3.365 ± 0.105

4.544 ± 0.144

5.728 ± 0.151

5.953 ± 0.286

6.978 ± 0.186

1.388 ± 0.078

3.987 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski