Gordonia phage Keelan

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ronaldovirus; unclassified Ronaldovirus

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VI27|A0A649VI27_9CAUD Uncharacterized protein OS=Gordonia phage Keelan OX=2656537 GN=62 PE=4 SV=1
MM1 pKa = 7.51AVTAKK6 pKa = 10.6VFGPFLNSMANKK18 pKa = 9.86EE19 pKa = 3.84VDD21 pKa = 4.08LNSDD25 pKa = 4.03TIKK28 pKa = 11.19VMLTTTAPDD37 pKa = 3.18QDD39 pKa = 2.8AWQYY43 pKa = 10.08KK44 pKa = 9.5SSVTGEE50 pKa = 4.17VTGTGYY56 pKa = 8.29TAGGATVASPTFSYY70 pKa = 9.04NTSTNVWMFDD80 pKa = 3.32GADD83 pKa = 3.83VTWSSSTITANYY95 pKa = 9.87AVFYY99 pKa = 10.34DD100 pKa = 4.11STPGTDD106 pKa = 2.82ATRR109 pKa = 11.84PLICYY114 pKa = 8.78WDD116 pKa = 3.65FGGAQSSSSGNFTLSFSASGLVTATVAA143 pKa = 3.78

Molecular weight: 15.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VIB3|A0A649VIB3_9CAUD Uncharacterized protein OS=Gordonia phage Keelan OX=2656537 GN=84 PE=4 SV=1
MM1 pKa = 7.68LVRR4 pKa = 11.84IQPCPPQGHH13 pKa = 4.84VTQLAEE19 pKa = 4.36VFSSRR24 pKa = 11.84HH25 pKa = 3.74EE26 pKa = 4.17TIAASLITRR35 pKa = 11.84IRR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 8.73STVAHH45 pKa = 5.94TLWITQVRR53 pKa = 11.84SLPGEE58 pKa = 4.05RR59 pKa = 11.84KK60 pKa = 9.5PFCCKK65 pKa = 10.18AFQIDD70 pKa = 3.61NPLWLSGRR78 pKa = 11.84AIHH81 pKa = 7.16

Molecular weight: 9.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

145

0

145

21107

32

1852

145.6

16.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.902 ± 0.485

1.36 ± 0.171

6.481 ± 0.209

7.178 ± 0.374

3.274 ± 0.142

7.917 ± 0.486

1.966 ± 0.148

5.117 ± 0.151

4.994 ± 0.269

7.832 ± 0.185

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.757 ± 0.131

3.994 ± 0.161

4.444 ± 0.161

3.539 ± 0.123

5.671 ± 0.218

6.382 ± 0.254

5.818 ± 0.273

6.931 ± 0.229

2.104 ± 0.137

3.34 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski