Salinibacillus kushneri

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Salinibacillus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0I1T0|A0A1I0I1T0_9BACI Menaquinol-cytochrome c reductase iron-sulfur subunit OS=Salinibacillus kushneri OX=237682 GN=SAMN05421676_110104 PE=4 SV=1
MM1 pKa = 7.73PKK3 pKa = 8.87YY4 pKa = 9.33TIVDD8 pKa = 3.71RR9 pKa = 11.84EE10 pKa = 4.12TCIACGACGATAPEE24 pKa = 4.24IYY26 pKa = 10.18DD27 pKa = 3.51YY28 pKa = 11.56DD29 pKa = 3.99EE30 pKa = 5.22EE31 pKa = 4.57GLSFCLLDD39 pKa = 3.73NNKK42 pKa = 10.09GISKK46 pKa = 10.15VPVDD50 pKa = 5.45LIEE53 pKa = 4.89DD54 pKa = 3.99LEE56 pKa = 4.58DD57 pKa = 3.58AHH59 pKa = 7.13DD60 pKa = 4.34GCPTDD65 pKa = 4.17SIRR68 pKa = 11.84IADD71 pKa = 3.98EE72 pKa = 4.35PFNGDD77 pKa = 3.25PDD79 pKa = 3.71KK80 pKa = 11.64FEE82 pKa = 4.04

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0CIW5|A0A1I0CIW5_9BACI Osmoprotectant transport system permease protein OS=Salinibacillus kushneri OX=237682 GN=SAMN05421676_103191 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.76KK9 pKa = 8.12RR10 pKa = 11.84KK11 pKa = 8.88RR12 pKa = 11.84NKK14 pKa = 8.51VHH16 pKa = 6.6GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 10.48NGRR28 pKa = 11.84KK29 pKa = 8.91VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.3GRR39 pKa = 11.84KK40 pKa = 8.62NLSAA44 pKa = 4.67

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3528

0

3528

977637

29

1504

277.1

31.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.358 ± 0.041

0.624 ± 0.012

5.427 ± 0.032

7.811 ± 0.055

4.533 ± 0.036

6.802 ± 0.046

2.156 ± 0.022

7.9 ± 0.048

6.91 ± 0.045

9.469 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.862 ± 0.022

4.726 ± 0.033

3.571 ± 0.024

4.134 ± 0.032

3.943 ± 0.033

5.986 ± 0.031

5.32 ± 0.025

6.844 ± 0.032

1.03 ± 0.016

3.594 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski