halophilic archaeon DL31

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; unclassified Haloferacales

Average proteome isoelectric point is 4.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3413 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2MKS8|G2MKS8_9EURY Uncharacterized protein OS=halophilic archaeon DL31 OX=756883 GN=Halar_1443 PE=4 SV=1
MM1 pKa = 7.41TFQLSWHH8 pKa = 5.88GHH10 pKa = 4.39STWTVTVDD18 pKa = 3.26DD19 pKa = 4.73TEE21 pKa = 4.26FLIDD25 pKa = 4.26PFFDD29 pKa = 4.4NPFTDD34 pKa = 4.82LDD36 pKa = 4.16PEE38 pKa = 4.26EE39 pKa = 5.44LDD41 pKa = 3.94PDD43 pKa = 3.98HH44 pKa = 8.01VLITHH49 pKa = 6.45GHH51 pKa = 5.75TDD53 pKa = 4.17HH54 pKa = 7.16IADD57 pKa = 3.43SDD59 pKa = 4.0RR60 pKa = 11.84FEE62 pKa = 4.29GTHH65 pKa = 6.26FVGTPEE71 pKa = 3.55ITGYY75 pKa = 8.77LTDD78 pKa = 5.01NYY80 pKa = 10.73DD81 pKa = 3.14IDD83 pKa = 5.04DD84 pKa = 4.1ATGMNLGGTVEE95 pKa = 4.6LGDD98 pKa = 3.94AFVTMVRR105 pKa = 11.84ADD107 pKa = 3.54HH108 pKa = 6.9SNGIDD113 pKa = 3.12TGYY116 pKa = 8.57GTSGGMPAGYY126 pKa = 9.86VISDD130 pKa = 3.75TKK132 pKa = 9.75PTQEE136 pKa = 4.24SDD138 pKa = 3.03ADD140 pKa = 3.73STTFYY145 pKa = 10.75HH146 pKa = 7.34AGDD149 pKa = 3.68TSLMSEE155 pKa = 4.11MKK157 pKa = 10.48DD158 pKa = 3.43YY159 pKa = 10.83IGPFLEE165 pKa = 4.81PDD167 pKa = 3.41AVAMPVGDD175 pKa = 4.8HH176 pKa = 5.4YY177 pKa = 10.22TMGPAQAAIAVDD189 pKa = 3.62WVDD192 pKa = 3.61PDD194 pKa = 3.63VAFPMHH200 pKa = 7.13YY201 pKa = 8.75DD202 pKa = 3.74TFPAIEE208 pKa = 3.9VDD210 pKa = 2.65IDD212 pKa = 3.42QFGRR216 pKa = 11.84EE217 pKa = 4.09VKK219 pKa = 9.79ATGNDD224 pKa = 3.13ADD226 pKa = 4.24VVVLDD231 pKa = 4.09GDD233 pKa = 3.65EE234 pKa = 4.76SYY236 pKa = 10.64TLAEE240 pKa = 4.14

Molecular weight: 26.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2MJP7|G2MJP7_9EURY PMT_2 domain-containing protein OS=halophilic archaeon DL31 OX=756883 GN=Halar_1355 PE=4 SV=1
MM1 pKa = 7.44GLHH4 pKa = 6.26RR5 pKa = 11.84MKK7 pKa = 10.7LGMVLAVGLGSLTFAQSLLTVGNPTARR34 pKa = 11.84QAVRR38 pKa = 11.84ALIGNATPRR47 pKa = 11.84VVTLL51 pKa = 3.92

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3413

0

3413

980990

30

1735

287.4

31.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.535 ± 0.055

0.768 ± 0.016

7.78 ± 0.045

8.94 ± 0.054

3.395 ± 0.026

8.392 ± 0.042

2.097 ± 0.023

4.123 ± 0.032

2.194 ± 0.025

9.053 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.799 ± 0.018

2.597 ± 0.024

4.659 ± 0.027

2.873 ± 0.022

6.338 ± 0.039

5.653 ± 0.026

6.348 ± 0.033

8.58 ± 0.041

1.167 ± 0.016

2.708 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski