Colletotrichum fructicola (strain Nara gc5) (Anthracnose fungus) (Colletotrichum gloeosporioides (strain Nara gc5))

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum; Colletotrichum gloeosporioides species complex; Colletotrichum fructicola

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17182 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7J6IPD5|A0A7J6IPD5_COLFN Putative membrane protein OS=Colletotrichum fructicola (strain Nara gc5) OX=1213859 GN=CGGC5_v012210 PE=4 SV=1
MM1 pKa = 7.29SKK3 pKa = 9.77IYY5 pKa = 10.57RR6 pKa = 11.84EE7 pKa = 4.23AEE9 pKa = 3.62RR10 pKa = 11.84VLFWLGPGTEE20 pKa = 4.43DD21 pKa = 3.85MMNAMLYY28 pKa = 9.07LQWLRR33 pKa = 11.84VKK35 pKa = 10.24VDD37 pKa = 3.15QVEE40 pKa = 4.27AHH42 pKa = 6.26DD43 pKa = 4.13HH44 pKa = 5.91FRR46 pKa = 11.84DD47 pKa = 3.43ILEE50 pKa = 4.65MGDD53 pKa = 3.63EE54 pKa = 4.27NQLEE58 pKa = 4.32LRR60 pKa = 11.84RR61 pKa = 11.84WIASKK66 pKa = 9.38LTDD69 pKa = 4.09AYY71 pKa = 10.91DD72 pKa = 3.96FSPYY76 pKa = 10.47EE77 pKa = 3.95FNLYY81 pKa = 10.73DD82 pKa = 4.07SDD84 pKa = 5.19PYY86 pKa = 11.59DD87 pKa = 4.04SDD89 pKa = 5.29PYY91 pKa = 11.5DD92 pKa = 4.03SDD94 pKa = 5.29PYY96 pKa = 11.5DD97 pKa = 3.91SDD99 pKa = 4.71PYY101 pKa = 11.21DD102 pKa = 3.7SEE104 pKa = 5.73PYY106 pKa = 10.33DD107 pKa = 3.62SEE109 pKa = 5.54PYY111 pKa = 10.39DD112 pKa = 3.62SEE114 pKa = 5.54PYY116 pKa = 10.41DD117 pKa = 3.58SEE119 pKa = 5.66PYY121 pKa = 10.71DD122 pKa = 3.97GDD124 pKa = 4.4SYY126 pKa = 12.0DD127 pKa = 3.37SDD129 pKa = 4.36SYY131 pKa = 12.06DD132 pKa = 3.48RR133 pKa = 11.84DD134 pKa = 3.88PYY136 pKa = 11.25SLEE139 pKa = 5.1ARR141 pKa = 11.84LKK143 pKa = 10.06WEE145 pKa = 4.12WPWDD149 pKa = 3.61HH150 pKa = 6.47EE151 pKa = 4.88QGFQEE156 pKa = 4.63LLLVPWFF163 pKa = 3.9

Molecular weight: 19.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7J6J4R5|A0A7J6J4R5_COLFN Uncharacterized protein OS=Colletotrichum fructicola (strain Nara gc5) OX=1213859 GN=CGGC5_v007109 PE=4 SV=1
MM1 pKa = 8.01RR2 pKa = 11.84YY3 pKa = 8.23STLTVLAIALLTGNALTAPTPQGGAGAIGGGNIGGGNNNGANNGGAGAGANNGQNQNNGQNQNNGQNNNQAPPPPANNNNNNNNNNQAPPPANNQNQNQNQQQQGNNNAAANNGANNNAVNNQANGANGATGAGRR138 pKa = 11.84NTAGNGNFRR147 pKa = 11.84RR148 pKa = 11.84AAPNQQPPPPPANNQTPPPPKK169 pKa = 9.74PNQARR174 pKa = 11.84AAPNQQPPPPPANNQTPPPPKK195 pKa = 9.74PNQARR200 pKa = 11.84AAPNNQPPPPPANNQTPPPPKK221 pKa = 9.74PNQARR226 pKa = 11.84AAPNQQPPPPPANNQTPPPPKK247 pKa = 9.74PNQARR252 pKa = 11.84AAPNQQPPPPPANNQTPPPPKK273 pKa = 9.74PNQARR278 pKa = 11.84AAPNQQQQPNQQAQAPPPPANNNNNTPPPPKK309 pKa = 9.82PNQARR314 pKa = 11.84AAPSQQPNQQAPPPPANNQTPPPPKK339 pKa = 9.74PNQARR344 pKa = 11.84AAPKK348 pKa = 9.23QQPPPPPPANQQVPPPAAII367 pKa = 3.88

Molecular weight: 37.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17182

0

17182

8073644

66

9872

469.9

51.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.076 ± 0.016

1.293 ± 0.008

5.819 ± 0.014

6.041 ± 0.02

3.847 ± 0.012

7.094 ± 0.02

2.322 ± 0.009

4.841 ± 0.013

4.784 ± 0.017

8.746 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.256 ± 0.007

3.67 ± 0.011

5.926 ± 0.024

3.863 ± 0.014

5.913 ± 0.018

7.788 ± 0.023

5.998 ± 0.023

6.324 ± 0.014

1.616 ± 0.007

2.783 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski