Brevibacillus phage Abouo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Abouovirus; Brevibacillus virus Abouo

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5MUL8|S5MUL8_9CAUD DNA N-6-adenine-methyltransferase OS=Brevibacillus phage Abouo OX=1296661 GN=ABOUO_79 PE=4 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84EE3 pKa = 3.45IKK5 pKa = 10.47FRR7 pKa = 11.84GKK9 pKa = 9.78PIEE12 pKa = 4.8DD13 pKa = 3.41YY14 pKa = 11.61GKK16 pKa = 8.28ITWFYY21 pKa = 10.76GSAILDD27 pKa = 4.02YY28 pKa = 10.23EE29 pKa = 4.58DD30 pKa = 3.41KK31 pKa = 10.86LAYY34 pKa = 9.61ILVSGQGVVPVEE46 pKa = 4.13WEE48 pKa = 4.07SVGQYY53 pKa = 9.93TEE55 pKa = 3.87VHH57 pKa = 6.87DD58 pKa = 4.94DD59 pKa = 3.68KK60 pKa = 11.48EE61 pKa = 4.51DD62 pKa = 3.72NEE64 pKa = 4.82VYY66 pKa = 10.69DD67 pKa = 3.99GDD69 pKa = 3.84IVEE72 pKa = 4.49ITHH75 pKa = 6.67EE76 pKa = 4.33GRR78 pKa = 11.84LLRR81 pKa = 11.84CYY83 pKa = 10.5VKK85 pKa = 11.14YY86 pKa = 10.35EE87 pKa = 3.86GSGFMLVSDD96 pKa = 4.54EE97 pKa = 5.83LEE99 pKa = 4.67DD100 pKa = 4.47GYY102 pKa = 10.88IWMSDD107 pKa = 3.71LIEE110 pKa = 4.63CDD112 pKa = 3.84RR113 pKa = 11.84SYY115 pKa = 11.58FWMPDD120 pKa = 3.15SIVIGNIYY128 pKa = 9.77EE129 pKa = 4.12NPEE132 pKa = 3.89VLEE135 pKa = 4.26VV136 pKa = 3.32

Molecular weight: 15.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5MCB3|S5MCB3_9CAUD Uncharacterized protein OS=Brevibacillus phage Abouo OX=1296661 GN=ABOUO_48 PE=4 SV=1
MM1 pKa = 7.3LTNLVNNSSTTPRR14 pKa = 11.84YY15 pKa = 9.09IHH17 pKa = 7.01LRR19 pKa = 11.84TLNFGWQKK27 pKa = 10.9KK28 pKa = 9.43KK29 pKa = 10.55PFTSAITEE37 pKa = 4.04KK38 pKa = 10.48TSVQKK43 pKa = 10.96YY44 pKa = 9.41RR45 pKa = 11.84GIKK48 pKa = 8.73KK49 pKa = 10.03RR50 pKa = 11.84RR51 pKa = 11.84VEE53 pKa = 4.35QIMQGG58 pKa = 3.27

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

13972

32

684

148.6

16.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.692 ± 0.342

0.752 ± 0.125

5.983 ± 0.242

8.066 ± 0.355

4.029 ± 0.22

5.976 ± 0.241

1.51 ± 0.1

7.15 ± 0.232

8.352 ± 0.285

8.395 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.67 ± 0.175

4.888 ± 0.251

2.977 ± 0.191

3.951 ± 0.207

4.967 ± 0.296

5.776 ± 0.218

5.733 ± 0.264

6.842 ± 0.227

1.331 ± 0.105

3.958 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski